EMAGE mouse gene expression spatial database

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EMAGE:29473

4930418P06Rik ( MGI:1924117)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004241_01	euxassay_004241_02	euxassay_004241_03	euxassay_004241_04	euxassay_004241_05

euxassay_004241_06	euxassay_004241_07	euxassay_004241_08	euxassay_004241_09	euxassay_004241_10

euxassay_004241_11	euxassay_004241_12	euxassay_004241_13	euxassay_004241_14	euxassay_004241_15

euxassay_004241_16	euxassay_004241_17	euxassay_004241_18	euxassay_004241_19	euxassay_004241_20

euxassay_004241_21	euxassay_004241_22	euxassay_004241_23	euxassay_004241_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6117
Entity Detected:	4930418P06Rik, ( MGI:1924117)
Sequence:	sense strand is shown >T6117 CTTAGAGAGAGTGCAGTTCTCCGTGTACCTCCCTGGGTTCCTAATCCTCTAGCACAGATCCTTATCCACG CTCCTGATATCCTGCAGTCATGCAGCGGAGAACGAGAGGGATCAACACCGGCCTCCTCCTGCTCCTCTCT CAAGTGTTCCAAATTGGGATCAACAACATTCCGCCTGTTACCCTCGCAACCTTGGCTGTCAACGTCTGGT TCTTCCTGAATCCTTGGAAGCCCCTGTACCATTCCTGCATCAGCGTGGAAAAGTGTTACCAGCAGAAAGA CTGGCAGCGTTTACTGCTCTCTCCCCTGCACCACGGAGATGACTGGCATTTGTATTTCAACATGGTGTCC ATGCTCTGGAAAGGTGTGAAGCTGGAGAGAAGGCTGGGAAGCCGATGGTTTGCCTACGTGATCGCCACCT TCTCCCTGCTTACGGGGGTGGTGTATTTGCTCTTGCAGTTTACTGTTGCCGAACTCATGAATCAGCCTGA CTTCAAAAGGAACTGTGCTGTGGGCTTCTCAGGAGTTTTGTTTGCCTTGAAAGTTCTTAGCAACCATTAC TGCCCCGGAGGCTTTGTCAACATTTTGGGCTTTCCTGTCCCCAATAGATTTGCTTGTTGGGCAGAGCTGG TGGCCATTCACTTCTGCACCCCAGGGACTTCCTTTGCTGGGCACCTGGCCGGCATTCTGGTTGGACTGAT GTACACGCAGGGGCCTCTGAAGAAAATCATGGACATGTGTGCAGGCATTTTTATCTCCCACTCTGGGCCT TCAGGACAGCAGAACCACTTTAACAATGCAGGCCCCTCCGGATATCAGAATCACTATGCAGATGGCAGAC CAGTGACCTATGATGCAACTTACAGGAACTATGATGTCTACACAGCGGGCCTGAGCGAGGAGGAGCAGCT GGAACGAGCATTAAGAGCCAGCATCTGGGACCGAGGAAATACCAGAAATGGTCCAATGCCGTATGGCTTC CGGCTCCCACCAGAAGAAATGAGGAGACAGCGTCTTCACAGATTTGATGGCCAGTGATATGGCACCATGC AGGGGAAGTCCAGCTGTGCGATTGGAGCCCGTTCACTTAACTCCCCAAGCCCCTAATTGGTTTTAAACAA AAGAAGAGCACAGGGTTACTGTTGCAGGTCACTCTTGTCTGCCATGAACAAGTACTCCATGGCCCCTGTG TGCCCAGATGCACAAGCCCAGCTGACATGTCCCAGGAAGCCCACAGCCTTCCCAGAGTGGCCTGTCTAGC CCCGTGATCGCTCCTAAACCGAAGGTCACCTCTTCAGATGGGACCAATTTCCATTTTTCTCCTTCCCTCC AGACTCAGTGGCTTATCCACACTGCGGTGAAGCCTGGGCCCTTAATAGGAAGTGGGTTCTTTATCTCACC AGGTGAGTGGATCAGCCCTCACTCTGCCTGGCATGCCAGGTTCTTGGCTTAGCAGGAGGGGCAGTGCTAC CCCTGGTCCTCCCACCATGTTTCCATGGTGATGCGGGAAGAGGATGTGGCTACCTTCCCAGGGCGTTCCG TTCTCTTTCACGCAGGCTCCTCCGGGTGCTCAGGCTACCAAGTCTGCCTGATGTTCACATCTCCTGCCTT ATTCCACATGTAGAATGTCTAACAGGAGGGGAATAAGAAAGATCTAAGGTAGGAAAAGAACCCAACTCAC TCATTAGATATCATTGTGGCACCAGCAATTTGGGTTCAGAGCCACCCCCTCCAGGAGCGGGCGACTTGTT TAACTGCTCCACAGAGCGGCAACAGGTGCCACACACACACAGAGCAAAGCCAACATGTTGAGTGGGGTTC TCACAGGGAATCCCCCTTGCAGCAAGTATTTTTTTTTTCCTTCATGAAAGTGCCTTCTCATTGGCTACTC TGGCTGTAGTTGGTCATAAACAAGTGTGTGAAAAATGCCCCAGGGAAATGCTGTGTCGAGTCACTTAAGG CATTTCTGTTTCTTGTGTTCTGAGAATAAAAAGGCAAAGACAGGGTGTTGCCACCTCCTCACAGTCAGGG AGGATTTAGATCAAAGGGAGTTAGGAGCAACAAATGGTGTTCCTCCGACTCTTATGCACCCTCCCTGCAT AGTTTCCAATGAGAATGGGTGTAGACAGGCTTTCCTATGCACAACGCACGCACACACCTAGGATGGAATA CTAAGTGGCGATACCCATCAGCTCTGGGGGACAGGCACATAGGACCTGACTTTCTTTGTTTACAGTTTTA TATTCTATTTTTTAAAGCGTGTGTGCAAGTGTCTGCTGCTTTCAGAAGATTACTTTATACAATGAATCAA GAAACATGTAGCCAGTAAAACAAACCCTAAGCTAAGTACGCTCTGTCCATTAAGAAAAATCATAGACTGG GCATGGTGGCACACATTTCTAATCCCAGCACTCAGGATTCAGAGGAAGGAGGATCTATGAGTTCAAAGCC AGCCTGGTCTACATATGGAGTTCTAAGCCAGCCAGAACACACACACACACCCTTATACACCAAAACCCTG TCTCAAAAAAGAGGGAGGAAGAGTGGGGGGGGAGAAGGAAGATGATGAAGGAAGGAAAGAAATCACAGTA TTTAAATAAAGGTACAACTGCTCATTTTTAAAAGGCACTGGTGCCCAAGGCCAACATTTCAAGGCTATTA ACATCTCAGTTTCTGCTGTATGCATTTGACACATCTGGTGCATGCCATCTGTGTAGCTCCATGCCCACTT GTTAATATGGACCCACCTGGAATGTCTATAACCCGTGCATATGAATGGACTAAGCAAATCCATGGAGTCT AAGCATTCAGACAATGGTGCCTGAGCACCTTAGTTTAATTTGTAATTGGTGGAAAGCCACACCTAGTTGA TATTATAAATTATGCCCATGAGCCTGGTACCTCCAAACACATTTAAATTATAATGGTCAAGGTAAATTAT CCCTGGCTCTCAGGCTGTACATTTGCCTTCCATACCACAATGAGAAACAACCTTTTCCCTACTTGACACC TTTTGATAACACCTGGGAATTCTACCCTAACACTCTTATGTCACTATTCTTATTTGTGCATACCTGCACC AAGTCATGTCTGTGGTTCTGAGTGACCCTAGACCACGGCCTGTTACTGACCTTCAACCTTCCCAGTCTCC CTCCCTCCACACTCAAAAGGCTCAGTAACTGTTATTTTCCCACCAAAGTTATTATTCACTTGTTATTAGA ATTCTGCTAGCTTGTACATGTAGCTTTAATGTAGTAACAGTACCTGGTTGCTTTGTTTGACTTGATGGTC AAAGACACTGAAAATCCCAATTTTGGATGGTGTAGTTATAAAATGCACTTTTTCTTTATTTATGGAGTGG GATGTCACCAAATGTTTATTACAGATGTGGGAAAAGTCATTTTAGGGATAAGTGAAAACCACATGTCTTC CAGAATAAAGATGGTCTGGAAATGATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3494511 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3494511 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004241 same experiment
	EMAGE:29470 same embryo
	EMAGE:29472 same embryo
	EMAGE:29487 same embryo
	EMAGE:29269 same embryo
	EMAGE:31358 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source