Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29562

D930005D10Rik ( MGI:2443088)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562
euxassay_002014_01 euxassay_002014_02 euxassay_002014_03 euxassay_002014_04 euxassay_002014_05
EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562
euxassay_002014_06 euxassay_002014_07 euxassay_002014_08 euxassay_002014_09 euxassay_002014_10
EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562
euxassay_002014_11 euxassay_002014_12 euxassay_002014_13 euxassay_002014_14 euxassay_002014_15
EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562 EMAGE:29562
euxassay_002014_16 euxassay_002014_17 euxassay_002014_18 euxassay_002014_19 euxassay_002014_20
EMAGE:29562 EMAGE:29562 EMAGE:29562
euxassay_002014_21 euxassay_002014_22 euxassay_002014_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2392
Entity Detected:D930005D10Rik, ( MGI:2443088)
Sequence:sense strand is shown

>T2392
TGGCCTCGAGGCAGATTCGGACGAGGATTCCTGAGGGCCATCTGGAAGGCCATGGCTGCCCCCTGGCCAA
TAGGGACCCAGGCAGAGTTGCTGTTGAAACTGCCCCTCCCCATTCTCTTCCTGTCACGGGAGCTCCCCGG
GCTCAGGGTCCCCCTGGAAGACAGCCTACCCGAGGGGACCGCAGGGGCTCCCAGGCTGGCTCTCTGCACA
CTGACTCCTCCTGCATGCTCACTCCGCCCAGCACGCCACTGGGCCTGGAGCCTGCAGGCCCCAGCTGGCC
AGAGCCCAGCGGCCTCTGTGGAAGAGCAGTCCTTGACGGGCAGAGGAATGGGCCAGGTGGTCTGCCTGGT
GCAGTCCTAGAAGGAGATGCGATTCAGGATGCTGAGCCTCCTGCTGAGGCCTCCAGCCCCAGCTCCAGCG
CTGAGGACTTCTGCTACGTGTTCATGGTGGAGCTGGAGCGAGGCCCCTCGGGGCTGGGAATGGGCCTGAT
TGATGGGATGCACACACCTCTGGGGGCCCAGGGACTCTACATACAGACCCTGCTTCCCGGGAGCCCCGCG
GCATCT
Notes:The probe template was PCR amplified from IMAGE:1195546 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1195546 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002014 same experiment
 EMAGE:31034 same embryo
 EMAGE:30125 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS