Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29568

Mcpt5 ( MGI:96941)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568
euxassay_001702_01 euxassay_001702_02 euxassay_001702_03 euxassay_001702_04 euxassay_001702_05
EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568
euxassay_001702_06 euxassay_001702_07 euxassay_001702_08 euxassay_001702_09 euxassay_001702_10
EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568
euxassay_001702_11 euxassay_001702_12 euxassay_001702_13 euxassay_001702_14 euxassay_001702_15
EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568 EMAGE:29568
euxassay_001702_16 euxassay_001702_17 euxassay_001702_18 euxassay_001702_19 euxassay_001702_20
EMAGE:29568 EMAGE:29568 EMAGE:29568
euxassay_001702_21 euxassay_001702_22 euxassay_001702_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2723
Entity Detected:Mcpt5, ( MGI:96941)
Sequence:sense strand is shown

>T2723
TGGCCTCGAGGCCAGATTCGGCACGAGGCTTACTCTTCATCTGCTGCTCCTTCTCCTGGGTTCCAGCACC
AAAGCTGGAGAGATCATTGGAGGCACGGAGTGCATACCACACTCCCGCCCCTACATGGCCTATCTGGAAA
TTGTCACTTCTGAGAACTACCTGTCGGCCTGCAGTGGCTTCCTGATAAGAAGAAACTTTGTGCTGACTGC
AGCTCACTGTGCGGGAAGGTCTATAACAGTCCTCCTAGGAGCCCATAACAAAACATCTAAAGAAGACACG
TGGCAGAAGCTTGAGGTGGAAAAGCAATTCCTTCATCCAAAATATGATGAGAATTTGGTTGTCCACGACA
TCATGCTACTGAAGTTGAAGGAGAAAGCCAAGCTAACCCTAGGTGTGGGAACCCTCCCACTCTCTGCCAA
CTTCAACTTTATCCCACCCGGGAGAATGTGCAGGGCAGTTGGCTGGGGCAGAACAAACGTGAATGAGCCA
GCCTCCGACACACTGCAGGAAGTAAAGATGAGACTCCAGGAGCCCCAAGCCTGCAAACACTTCACCAGTT
T
Notes:The probe template was PCR amplified from IMAGE:1513042 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1513042 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_001702 same experiment
 EMAGE:31774 same embryo
 EMAGE:29574 same embryo
 EMAGE:29721 same embryo
 EMAGE:31720 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS