EMAGE mouse gene expression spatial database

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EMAGE:29603

Pde4dip phosphodiesterase 4D interacting protein (myomegalin) ( MGI:1891434)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006106_01	euxassay_006106_02	euxassay_006106_03	euxassay_006106_04	euxassay_006106_05

euxassay_006106_06	euxassay_006106_07	euxassay_006106_08	euxassay_006106_09	euxassay_006106_10

euxassay_006106_11	euxassay_006106_12	euxassay_006106_13	euxassay_006106_14	euxassay_006106_15

euxassay_006106_16	euxassay_006106_17	euxassay_006106_18

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9657
Entity Detected:	Pde4dip, phosphodiesterase 4D interacting protein (myomegalin) ( MGI:1891434)
Sequence:	sense strand is shown >T9657 GCGTCAGCTCTGAGCCGACTTTACTGTCCCAGAGCCTAGACTGCCCGCCCCGGAGGACGTGGAGGACGAG GCAGCCCGCGGGAGAGACAGCTGTGGGGGCCCTGGCAGCGACCTCGCGCAGCTCGTACCCTGTCACTCAG CATCCCAATCGGTGTTGCGCCCGCGCCCGCCCCCTGCCCGAGGGGCGGGGCTGGTCTTGTGGTACCCAGA GTCTGCGCACGCTGAGGTGTGCCCCTGCCCAGTGAGGACCTGGGACTGGGCCATGCCAGCCCCAGACCAT CAAGGTGGGAAGAGGACCGCATCCCAGAGCCGGTGAGCAACCCGCAGCTTTGGCCGCTCCAGAGAAGTTC CGCACCCTCCAGGAGTGAAGATGAAAGAGATTTGCAGGATCTGTGCCCGGGAGCTGTGTGGAAACCAGCG GCGTTGGATCTTTCATACCGCGTCCAAGCTCAACCTCCAGGTTCTGCTTTCGCACGTCCTAGGCAAAGAT GTCTCTCGTGATGGCAAAGCCGAGTTTGCTTGTAGCAAGTGCGCTTTCATGCTCGATCGCATCTATCGAT TCGATACTGTCATTGCCAGGATCGAAGCCCTTTCTCTTGAGCGCTTACAGAAGCTGCTCCTGGAAAAGGA TCGCCTCAAGTTCTGCATTGCCAGTATGTATCGGAAGAATAATGATGACTCTGGCGAGGAGAACAAGGCG GGGAGTGGGACAGTAGACATTTCCGGCTTGCCAGATATGAGGTACGCTGCGCTGCTCCAAGAAGACTTTG CCTATTCGGGATTTGAGTGTTGGGTAGAAAATGAGGATCAGATTAACGACTCACACAGCTGTCATGCTTC CGAAGGGCCCGGAAACCGACCCAGGAGATGTCGCGGTTGTGCAGCTCTGCGGGTTGCGGATTCCGACTAT GAAGCCATTTGTAAGGTGCCTCGAAAAGTGGCCCGAAGTATTTCTTATGCCCCCTCTAGCAGGTGGTCTA CCAGCATTTGCACTGAAGAACCGGCATTGTCAGAGGTTGGGCCACCAGACTTAGCAAGCACAAAAGTACC CCCGGATGGAGAAAGCATGGAGGAAGGGACACCAGGTTCCTCCGTGGAGTCTCTGGATGCTAGTGTCCAG GCTAGTCCTCCACAACAGAAAGACGAGGAAACGGAGAGAAGTGCGAAAGAACTTGTAAAGTGTGACTACT GTTCTGACGAACAGGCTCCACAGCATTTGTGTAACCACAAACTGGAGCTAGCTCTTAGCATGATTAAAGG TCTTGATTATAAGCCCATTCAGAGTCCCAGAGGGAGCAAGCTTCCTATTCCAGTGAAATCCATCCTACCT GGAGCCAAGCCTGGCCATATCCTGACAAACGGAGTTAGTTCCAGTTTCCTTAACAGGCCTTTGAAACCCC TTTACAGGACGCCTGTGAGCTATCCTTGGGAGATTTCAGACGGACAGGAGCTGTGGGATGATCTCTGTGA TGAGTATTTGCCGATCGGGTTCCAGCCCGTGCCTAAAGGGTTACCCACGCAACAGAAGCCGGACTTGCAT GAGACCCCTACAACCCAGCCACCTGTGTCTGAGTCCCACCTGGCAGAACTCCAGGACAAAATCCAGCAAA CAGAGGCCACCAACAAGATTCTTCAAGAGAAACTGAATGACCTGAGCTGTGAGCTGAAATCTGCACAGGA GTCATCTCAGAAGCAAGATACGACAATCCAGAGCCTCAAGGAAATGCTTAAAAGCAGGGAAAGTGAGACT GAGGAGCTGTACCAGGTGATCGAAGGACAAAATGACACAATGGCAAAGCTTCGGGAAATGCTGCACCAGA GCCAGCTCGGACAGCTCCACAGCTCAGAGGGCATTGCCCCTGCTCAGCAACAGGTAGCCCTGCTTGACCT TCAGAGCGCTCTATTCTGCAGCCAGCTTGAAATACAGAGGCTCCAGAGGCTGGTCCGGCAGAAAGAACGC CAGCTGGCGGATGGCAAGCGATGTGTGCAGTTAGTGGAGGCTGCAGCCCAGGAGAGAGAGCACCAGAAGG AAGCTGCTTGGAAACATAACCAGGAATTACGAAAGGCTTTACAGCACCTCCAAGGAGAACTGCACAGCAA GAGCCAGCAGCTCCATGTTCTGGAGGCGGAAAAATACAATGAGATTCGAACCCAGGGACAAAACATCCAA CACCTAAGTCACAGTCTGAGTCACAAAGAGCAGCTAATTCAGGAACTCCAGGAGCTCCTACAGTATCGTG ACAACGCAGACAAAACTCTAGACACAAACGAAGTGTTTCTTGAGAAATTGCGGCAACGAATACAAGACCG AGCTGTTGCTCTAGAGCGGGTCATAGATGAGAAGTTCTCTGCTCTAGAAGAAAAGGACAAGGAACTGCGG CAGCTTCGCCTAGCTGTGAGGGACCGAGACCATGACTTAGAGAGACTGCGTTGTGTCCTGTCCGCCAATG AAGCTACCATGCAGAGCATGGAGAGTCTCCTGAGGGCCAGAGGCCTGGAAGTGGAGCAGTTAACTGCCAC CTGCCAAAACCTCCAGTGGCTGAAAGAAGAACTGGAAACCAAATTTGGCCATTGGCAGAAGGAACAGGAG AGCATCATTCAGCAATTGCAGACATCTCTACACGACAGGAACAAAGAAGTAGAGGATCTCAGCGCAACTC TGCTCTGTAAACTTGGACCGGGTCAGAGTGAAGTAGCTGAGGAACTGTGCCAGCGCTTGCAGCGAAAGGA ACGGATGCTGCAGGACCTTCTGAGCGATCGGAACAAACAAGCCGTGGAGCACGAGATGGAGATCCAGGGG CTGCTCCAGTCGATGGGCACCAGGGAGCAGGAAAGACAGGCTGCTGCAGAAAAAATGGTCCAGGCCTTCA TGGAAAGGAACTCAGAACTGCAGGCCCTGCGCCAGTATTTAGGGGGGAAGGAACTAATGACATCGTCTCA GACGTTCATCTCTAACCAGCCAGCTGGAGTGACGTCCATCGGGCCTCACCACGGAGAGCAAACCGATCAA GGTTCTATGCAGATGCCCTCTCGAGATGATAGCACCTCACTGACTGCTAGAGAGGAGGCCAGCATACCCC GGTCCACATTAGGAGACTCGGACACAGTTGCAGGGCTGGAGAAAGAACTGAGCAACGCCAAGGAGGAGCT CGAGCTCATGGCCAAAAAAGAAAGAGAAAGCCAGATGGAACTGTCTGCCCTGCAGTCCATGATGGCCATG CAAGAGGAAGAGCTGCAGGTGCAGGCTGCTGACTTGGAGTCCCTGACCAGGAATGTGCAGATAAAAGAAG ATCTCATAAAGGACCTGCAGATGCAACTGGTCGACCCTGAAGATATACCAGCCATGGAGCGTCTTACCCA AGAGGTCTTACTTCTTCGGGAAAAAGTTGCTTCCGTGGAACCCCAGGGTCAGGAAGTATCAGGGAACAAG AGACAGCAGTTGCTGCTGATGTTAGAAGGACTAGTGGATGAACGGAGTCGGCTCAACGAGGCCCTGCAAG CTGAGAGGCAACTCTACAGCAGCCTGGTCAAGTTCCATGCCCAGCCAGAGAACTCTGAGAGAGACGGAAC TCTGCAGGTGGAACTGGAAGGGGCCCAGGTGTTACGCACTCGACTAGAAGAAGTTCTTGGAAGAAGCCTG GAGCGTTTAAGCAGGCTGGAGAGCCTGGCCGCCATTGGAGGTGGGGAACTGGAAAGTGTGCAAGCCCGTC ACAAGCATGCCTTCTGAGCACTGGCGGGTCACACTGCGACCCAGGATGGAAACCCTGTTTATACTAACCA GAAAGCTGTGTCGGACTTGACAACCAAGTGTGGCTTACTAATGGTAACGGTAGGACTGCTGTCAGTAGCA GCAAAGGGAAGCAGCTGGAGTCTTCCTTACTCCCTGCACGGACCTAGTTCTTAGCTAACTGAGGTCTTGA AGCATACTGCACATCTTAAATCATTCCATTTTAATTTCCCATTCATACTGCTCTTCTCCCTGGCCTGTCC TCATTCCTTTACCCCTTCCATGGGCCAATAAAGTTTACTCCCCCATCTTACCACCCCCGCCACAAGTCCC ACGTCATGTCATCAAATGCATGTGTGTGTATTATTTTTGCCATCCAATCAAATCCTGTTCTTTGAAAGCA ATTTTTAATCAAATAAAGGAATCAAGTATGAGTTTTAGGTTTAGAAAGAAAGGTAGAGAGTCCCGTGAGA GTGAGTCTGGGGAGGGGTATAAAACCCTACTAAGGATAATCAGAACTAGACAGGAGGGTGTGGGCTGAGC TCAGGAGCAGAGACCGTTAGTGTTCCAATAAAATTTGCCCTCGAAGGCCTCTTCAGAGTTGACTGTCTAG GGAAAGATGAAGTCAGATCAGTGGACAGCATGGTCTAGTCCATAAAAGCCTATACAACTCATGTTCTTCA CTGGCCTAGGGTTTCTTCAAGCCTCAGGCATGGCCTTTAGTCAGAACATGACTTTAAAAACCGGTTTCCC AAATCAGAATCCTCTAGATAAAAAATCATCTTCTATGTGATATTTCCCACCCTCCTTTTTTTTATTATTT TGGTTTTGTTTTTTTGGTATTTTATTTGTTTGTTTGAGACAGGATATCACTGTGGCTGGCCTGAAATGCA CTGGGTAAACCAGACTGGCATTGAACTCACAGAGATGAGCCTGCTTCTACCTTTGGGTGCTGGGATTAGA GGCATGGCTGCCACCCCTGCCTGTCAGCATCCCCCTTAAGAGCAATAGTTTCCAGGATCCCCAGATCAGG GGAAGTATAATTCCTGGATGCTCATTAGGAGGTGAAGCGGGTGGCGTCCTTCATTGACCTCTGCTTTGGT GGGAGTAATTCTTGATAGTGGAGTAAGAAGACGATGTGAGATGCTGTCATGTTCAGGTCACTCAGAAGGA GGCCTTAGATCTCATGTAGGTTTTACTCACCCTCTCTGCTTTCTTGGGACAACCCGACAGGTGGGGAGGT GGTAGATTGTTCATTTCTAGCATCTTAAATGTCAAGAATAGGAAAAGTAACAGGTTACTTGCAAGGGGCT GTGGATTCTGCCCCAGAACCTTTCCTAGTGCTGAAGGGTATGAGTAACGTAATCCATGCTTTCCAGCACC CTGAGAAAGACTGAGCCCCCTTCCCGAGCTACACTGAGCTTTCCTTTCCTCTTCTTATTTATTTTTTATT TTTTATTTTTTTGACAAGGCACACTACATCCATGTGTTGACATGGCATGTGTTCTAAATTCTATTCAAGA TTCTGTAACCTCAGCTGTGTGACACACTTTAACTGATGTGTATCCATCAGCTTGAAGTGGACAGGAGGAA AATATTCTACCAGAGAAGCACTTGAAATCTTCTGATGAAGTAGTTCACAAAGATTGATAGCCATGGCCTT CCTTATAATGGATGTGCTATTACAGTTTTACAACCTTTAGGGGGGAAAGGACTCATTTAATATTAAAGAT AGAAGATGTAGAAGCAGAGCCATCCAATGTTCTTAGTAACCCCATTCTAAGATACTCTAAGGCCTGCCTG AACAAACCTTATGTAACTAACAAGGAAGAGCATAATAGAGTTGAGCTATAGACATGTCAAACAATTAAAG ACCAGCCTGTTAGTCATTACAAGGCAATTAGGAAATGTGTTTACTCAACTTTACCAATAGACAACAAAGT TCTGCAAAGCTGCTATTAAGTTTCAACTCTAGTAATCTCTAGGGTTCTGAGGGCTCACAGTGATTATAAT AATGAAGGTTAATAATTAGTGCAACTGCCAACCTCTAACAAGAGTAGGTTGATAGACAAGTAAATTAAGA GTTTGTTATCAATTCCATAAATGTGACTATAAAATCTGGTACTGACTTTCTGGTCCTGATGCTAGAATGA AGGTGGAGACCTTGCTGCCTGGAGGGAATGTGCTTGGCCCACAAGCTTGTGCAATAATTTGACACCTAGC TACCGAACACAGTTCTGATGAATTGTACAGCGTGAGCCACAGGTGGATGGTACTATGATTACACGTCATT CATATCATATAATTGGATTTAGCATGCTGTAATCTTCATTTCTCTGTAGGAGTATGGACTATGTTAGAAT GTGTCTGCCTTTGTTTGGATTTTTTTAATATTATAATAAAATAAACTTAGTTTTAAAACAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6591150 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6591150 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006106 same experiment
	EMAGE:29607 same embryo
	EMAGE:29588 same embryo
	EMAGE:29612 same embryo
	EMAGE:31544 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source