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EMAGE:29704

Cspg5 ( MGI:1352747)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29704 EMAGE:29704 EMAGE:29704 EMAGE:29704 EMAGE:29704
euxassay_000401_01 euxassay_000401_02 euxassay_000401_03 euxassay_000401_04 euxassay_000401_05
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euxassay_000401_06 euxassay_000401_07 euxassay_000401_08 euxassay_000401_09 euxassay_000401_10
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euxassay_000401_11 euxassay_000401_12 euxassay_000401_13 euxassay_000401_14 euxassay_000401_15
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euxassay_000401_16 euxassay_000401_17 euxassay_000401_18 euxassay_000401_19 euxassay_000401_20
EMAGE:29704 EMAGE:29704
euxassay_000401_21 euxassay_000401_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3779
Entity Detected:Cspg5, ( MGI:1352747)
Sequence:sense strand is shown

>T3779
TGGCCTCGAGCCAGATTCGGACTAGGGGGGCTTTCTGCAGGTGTAACACCCAGGACTACATCTGGCACAA
GGGGATGCGCTGTGAGTCCATCATCACGGACTTCCAGGTGATGTGCGTGGCCGTTGGCTCGGCTGCTCTC
GTGCTTCTCCTCCTGTTCATGATGACTGTGTTCTTTGCCAAGAAGCTCTATCTGCTCAAGACTGAGAATA
CCAAGCTGCGGAGGACCAATAAATTCCGGACCCCATCTGAGCTCCACAACGACAACTTCTCCCTCTCCAC
CATTGCCGAGGGCTCTCATCCAAATGACGACCCCAGCGCTCCCCACAAAATCCAGGACCCTCTCAAGTCC
CGCCTGAAGGAGGAAGAGTCCTTTAACATCCAGAACTCCATGTCACCCAAACTTGAGGGTGGCAAAGGTG
ACCAGGATGACTTGGGGGTGAACTGTCTGCAGAATAACCTAACCTGAGACTGAGGAAGAAGAGAGGAAAG
GGGGGTGGGGGAGGGAAGGACTGTTGTCTCCTCTCGGGCAGAGTCGGCTTCTTGTAACCATTTGTTAAGC
T
Notes:The probe template was PCR amplified from IMAGE:389173 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:389173 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_000401 same experiment
 EMAGE:30108 same embryo
 EMAGE:29790 same embryo
 EMAGE:31971 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS