EMAGE mouse gene expression spatial database

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EMAGE:29769

Slc2a13 solute carrier family 2 (facilitated glucose transporter), member 13 ( MGI:2146030)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5358
Entity Detected:	Slc2a13, solute carrier family 2 (facilitated glucose transporter), member 13 ( MGI:2146030)
Sequence:	sense strand is shown >T5358 AAAAAAAATTTTTGTTGCGTGTTGAGCATGTAGCACATACGGCTGGAATGATGTCAAAAGACTTGATGTT GTGATCTTGGGAGACAGTTCAGTGTTACAGCGCTGGCCTGTAAAGATTGGGCCTTAATATTTTTCTTCCA TATAGACATCTGTAATAATTTTGCTGGCTACACCTATATTGGTCAGCATCCAAAACATGGCTTTGAATAT ACCTGGAGAAGATCTAAATAAATAGTAATTACAGGAGTAAAACCACCAGAATCTTTTCAACAGTTTTATC AATGAAACACACTTTAGGTCCAGTCTTAACAACTGCCACTCACTCTAATGTGGGAGACCAGTGAAAGCAC AGAATTTATGGATCTTTGCTGTAACTCCAGTGTCCACTAAACAGAGAGAAAGACTGGGGAGGAACTGACA CCTGACCCATTTAGTTTTTAAGAGCAAAGTAAAACTGTAATGCATTGTTAATAATCATACCTCTCGGGGC ATGGGTGAGGTGGTGTTTTAGACAGCGCGACCCATGTCTTCTGTAATAACCGTGTGTGAAAATGTTTCAA TTATCAAGTTTGGACCATTTCCCTAACTAGAAACAAAAGGATTAGAAGGTAAAAACCACGCCAAGGGTAG AGAGTTCCTGGCAGCTGCTCTCTAGCAAACAGTTCTACATTCTACCTTAAACTGCAAAGTTAGAGATGAA ATTCACAGGTCAAAAGTAGCGACTGCCTATAAAAGGACTCAGAATTATTCCTCTGGGTTAATTTTTAACA TCCTTTCAGCCTAGAAATCTCTACCAATCCTGCCTCGTGCACAGCCAGGGTTCATTCAGTTCTCCAGTAG TGCTGCTTCTTGGGAAACCACAATCAGCTGCCAATGCTACAGCTAGCTACTGCTCACTCTTCTTGTATGC CAGTTAGAGAAAGACAGTGCGTAA
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002584 same experiment
	EMAGE:29615 same embryo
	EMAGE:31631 same embryo
	EMAGE:29630 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source