EMAGE mouse gene expression spatial database

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EMAGE:29811

1110032A13Rik ( MGI:1915981)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001698_01	euxassay_001698_02	euxassay_001698_03	euxassay_001698_04	euxassay_001698_05

euxassay_001698_06	euxassay_001698_07	euxassay_001698_08	euxassay_001698_09	euxassay_001698_10

euxassay_001698_11	euxassay_001698_12	euxassay_001698_13	euxassay_001698_14	euxassay_001698_15

euxassay_001698_16	euxassay_001698_17	euxassay_001698_18	euxassay_001698_19	euxassay_001698_20

euxassay_001698_21	euxassay_001698_22	euxassay_001698_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3069
Entity Detected:	1110032A13Rik, ( MGI:1915981)
Sequence:	sense strand is shown >T3069 TGGCCTCGAGCCAGATTCGGACGAGGTGCTCTCTCCTCAGGCATCTTCTGATATCTCAGCAAACCCTGAG AAATGTTCCACCCATAGTGTTTGTTCAAGACAAAAGAGACTTGGTCCTGGCTGAGGTAGATCGGTTGCTG GCAGAGGCTGACTTTGGACCCCCAGATGAAAGGGACGACTTGGACGGTTTGAGGAACGCCGATGCCCAGG TACCACATGACTCCCCAGAACCAGCCGCACACCCAAATCTGTGTGGGATTGACCACGAGGCGCTGAATAA GCAAATAATGGAGTACAAACGCAAGAAGGAGCGAGGGCTTCAGTGTCAGAGTCTGGCGCCACCATCAGGG CGGGAGCAGGCACCTGAACCGACCCGTCTGCTCCGGAAGAGAGAGAAGGTGCGATCCCGGTGGCAGCACC GGGACGCTTCCCCCAGAAGCTTCCTTTTGGGCGAGGAGGACGAGGACGAGGACAGCAGCACAGAGTGGGA ATGCCACGCCCACGAGGCAGAGGATGATGATGATCATCAGGAAGAGGAGCTCGGA
Notes:	The probe template was PCR amplified from IMAGE:1546662 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1546662 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001698 same experiment
	EMAGE:29573 same embryo
	EMAGE:29572 same embryo
	EMAGE:31520 same embryo
	EMAGE:31513 same embryo
	EMAGE:31974 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source