EMAGE mouse gene expression spatial database

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EMAGE:29839

Wig1 ( MGI:1195270)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001674_01	euxassay_001674_02	euxassay_001674_03	euxassay_001674_04	euxassay_001674_05

euxassay_001674_06	euxassay_001674_07	euxassay_001674_08	euxassay_001674_09	euxassay_001674_10

euxassay_001674_11	euxassay_001674_12	euxassay_001674_13	euxassay_001674_14	euxassay_001674_15

euxassay_001674_16	euxassay_001674_17	euxassay_001674_18	euxassay_001674_19	euxassay_001674_20

euxassay_001674_21	euxassay_001674_22	euxassay_001674_23	euxassay_001674_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3148
Entity Detected:	Wig1, ( MGI:1195270)
Sequence:	sense strand is shown >T3148 TGGCCTCGAGGCAGATTCGGACGAGGGTGGCTGGTCGTTGTTCTGCCTTCTTTCAGTATCTGATCACCCA CCAAAGGTTCTGTAAGCCAGTGTGTCACTTTGTAAGTCTCACGTTGACTCTGCTCACCTGTGGCCCCCCA CCCCCCACCCCCAGTCAACTGTAGTGACCTGGCATGGGTTTTGTGTGTTGCCTTCGAAATGCCTTATTGG GTATGCCAGGCTACTCTGTCAAGCATCCCAGCTGTCTGCTGAGACATACATGTCCTCTCTGGGGTATGTG CTCTTGATCCAGAAGCAAAGCTGATCTTGCTTTCAGTGTCAAGGGTGCGTTTTAATTCAGTTATTGTCTT AAGTTAGAAGTGAAGCAGGAAGCCGAGAGAATGTGAACCTTTCACGTGATCAGTGTGGCAAACCGAAAAT GTGCTTTTGCTGTTTCACTAGAAAGAACGCAGCTAAGAAATGCCAGTCCAAACCGGAAAGTGGGGCTGTG TCATATCACGCTCCCCACTTGTCACTTGGCCGTTGAGTGTCTGAGCAGGCCA
Notes:	The probe template was PCR amplified from IMAGE:1547874 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1547874 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001674 same experiment
	EMAGE:29936 same embryo
	EMAGE:29938 same embryo
	EMAGE:32191 same embryo
	EMAGE:29943 same embryo
	EMAGE:32214 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source