Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:29904

Ltk ( MGI:96840)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904
euxassay_016319_01 euxassay_016319_02 euxassay_016319_03 euxassay_016319_04 euxassay_016319_05
EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904
euxassay_016319_06 euxassay_016319_07 euxassay_016319_08 euxassay_016319_09 euxassay_016319_10
EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904
euxassay_016319_11 euxassay_016319_12 euxassay_016319_13 euxassay_016319_14 euxassay_016319_15
EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904
euxassay_016319_16 euxassay_016319_17 euxassay_016319_18 euxassay_016319_19 euxassay_016319_20
EMAGE:29904 EMAGE:29904 EMAGE:29904 EMAGE:29904
euxassay_016319_21 euxassay_016319_22 euxassay_016319_23 euxassay_016319_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45122
Entity Detected:Ltk, ( MGI:96840)
Sequence:sense strand is shown

>T45122
AACAGGTCACTGGAGGGTCTTAGATCCCCAAAGCCCCTAGAGCTGAGTTCTCAGAACTTGAAGAGCTGGG
GAGGAGGCCTTCTTGGCTCTTGGCTGCCCTCTGGCCTCAAGACCCTCAAACCCAGGTGCCTCCAACCTCA
GAACATTTGGAACCCCACCTATGGCTCCTGGACCCCAAGGGGCCCCCAGGGTGAAGATACAGGCATTGAA
CACTGCAATGGCTCCTCCTCAAGTTCCATTCCAGGCATCCAGTAGGCTCTGCCCCTGTCCCTGTGCTGCA
TGTTCAGGCATGTTTCAGGGATGTGCGGGCAACCTGGCCTCCCACACTGGAAACTAGCCCAGACCCTCTT
GGGGAAGGTCCTAGGCTACTTTCAGTCTTTGGTCTTTGAAACCAGAGGCCATCCACCCACTGCAGGAGTC
AGTTGGGAATCCTGGATTGTCCACTCCTCATAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 224829224829. Forward Primer - name:224829_F_exon_Ltk, sequence:AACAGGTCACTGGAGGGTCTTA; Reverse Primer - name:224829_N_SP6_exon_Ltk, sequence:CTTATGAGGAGTGGACAATCCAG.The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_016319 same experiment
 EMAGE:29858 same embryo
 EMAGE:32084 same embryo
 EMAGE:29865 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS