EMAGE mouse gene expression spatial database

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EMAGE:29923

Tpt1h ( MGI:1333115)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_004303_21	euxassay_004303_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8261
Entity Detected:	Tpt1h, ( MGI:1333115)
Sequence:	sense strand is shown >T8261 GCAAGTCTCGCCCGCTGGGCCTCAAGTTCCCTTGCTGAACTAGGGTGGGATGAGCAGTGCGGTTCTTATC CTGAGCTTTTGTAACTTTCTGGGCAGAGAACCCACACTCCCTGCTCCCTACAGGTCTTGATCATGAACGC CCCTGGAGGAAGGAGGAAGGAAGGTAGAAGGACTCATAGACCCCGGGAACAGGACCGAAATGTGCAGCTG TCTAAGGCTCTGTCCTATGCCCTTCGCCACGGGGCCTTGAAGCTGGGACTTCCCATGCGAGCTGATGGCT TTGTGCCCCTGCAAGCCCTCCTGCAGCTGCCCCAGTTCCACAGCTTTTCCATTGAAGATGTGCAGCTCGT GGTCAATACCAACGAAAAGCAGCGGTTTACCCTGCAGCCAGGCGAGCCGAGCACTGGGCTCCTCATCAGG GCCAATCAGGGCCACTCCCTACAAGTCCCTGAGTTGGAACTGACGCCCCTGGAGACACCACAAGCCCTGC CCTTGACACTAGTCCATGGTACATTCTGGAAGCACTGGCCATCTATTCTACTAAAGGGCTTGTCACGCCA AGGAAGGACACACATCCACTTGGCCTCAGGATTGCCTGGGGACCCTGGTGTCATCAGCGGCATTCGTCCA AATTGTGAGGTGGCGGTGTTCATCGATGGACCCCTAGCCCTCACAGATGGAATCCCTTTCTTCTGCTCCG CCAATGGTGTGATTCTGACTCCAGGGAATGCTGAAGGCTTCCTGCTTCCCAAGTACTTCAAGGAGGCCCT GCAGCTTCGACCTACACGAAAGCCTCTCTCCTTGGCTGGTGATAAAGAGACAGAGACTCAGAGTGGCCCC AAGCTCAGTTCCAGAGGAGGAAGAAGAAAGATCCAACAATAAAATATTTATTTATTAAAAAACCTGAAAC AGACACAGAAAGAAAGTCCAGCTTCAAACCAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1364225 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1364225 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004303 same experiment
	EMAGE:32210 same embryo
	EMAGE:29925 same embryo
	EMAGE:29535 same embryo
	EMAGE:30885 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source