EMAGE mouse gene expression spatial database

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EMAGE:29935

4833432M17Rik ( MGI:2685830)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_004315_11	euxassay_004315_12	euxassay_004315_13	euxassay_004315_14	euxassay_004315_15

euxassay_004315_16	euxassay_004315_17	euxassay_004315_18	euxassay_004315_19	euxassay_004315_20

euxassay_004315_21	euxassay_004315_22	euxassay_004315_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5885
Entity Detected:	4833432M17Rik, ( MGI:2685830)
Sequence:	sense strand is shown >T5885 CCGGGAAGATGGCGGCTGTCTCTGAAACAGTGCTCGTCTCCGCCCCGCAGGACCACGATGCCCAGGCCGC CTCAGACCCTCAGGCCACGGCAGCAGACTCTCCCCTAGAGGACTTCCGAGTGCGCTGCACCTTGAAGAGG GCGGTGATGGAAGTGATGGAGATGTGCGGTCGCTTCGTGCAGGAGCTCGGGGCCGTGCTGCCGGAGGACG TCCGCGAGCTCGCGCTGCGGGACGCACAGTGGACATTTGAGTCGGCTGTGCAGGAGAACGTCAGCTTTAA CGGACAGGCCTGGGAGGAAGCTAAAGAGCATGGCTTAATGGATTCTGACATCAAAGTGCTTGAAGATGAA TTTGATGAGCTCATAGTAGATGTGGCAACCAAACGGAGGCAGTATCCCAGAAGGATCCTTGAGAGTGTCA TCAAAACCTTGAAAGCACAGCACGCGAGTCTGAAGCAGTATCACCCTGTTGTACATCCTCTGGACCTGAA ATGTGACCCTGACCCAGCTTCTCGTGTAGAAGATTTGAAATGCCGCGGAGAAGCCATAGCTAAGGAAATG AGTGAGGCCATGAAGGCTTTGCCTGTGCTAATTGAACAAGGAGAAGGCTTTTCCCAGGTCTTAAAGATGC GACCTGTCATCCAGCTACAGAGGATCAACCAGGAAGTCTTCTCCAGCCTCTACAGGAAAGCTGACTTCAA GCCTGACACATGTGTGACACACGTAGAAACCACACCAGCCGAGACTGGAGCCAGGAAGGCCTCCAACATT GTGCTAAAGAGAAAGAAAGCCCCAGACTGTGCCCAGAGAAAGCGCTACCCGCTGCGGCTGCAGAGAATTA ATCTCGATATGTGAGCTCTGTTTGTTGAGGAGCTGAAAGTCAGTGTTGTCAACATTTGGGTCACAGTCTG ATGCCTGAGTGACTTCATGAAAGTGAGTGACTCTGTCCAGGCACTGTACAGGTGCAGTGCGCTGCTGTGA ATCCTGGAATACGGCTGAGTGCCTAGCTTCTTCCCTTGAGTCCCTGTGCAGTAGGTGAAGACTTCCCAGT CCTGCACAGACCTGTCGGTAGCCTCCGATGACACCAGCTCTTGGCTTTGGCTGCCTTGCAGCTTTGAGGC ATCCCAGGGATGTGCTCTGAGGGGTTTTGTTTTTAGCACAGCTTTCTGTGAGGCTCTGAGGTGGTTCCCT GTGGCTGGATAGCTGGCATTGGCTCTGCGTGGCCGTGTGTGCATTTCCAGGCAGGTTTTCAGATGAATGC ACAGAAAATGGGCCTGGCCTTAAGTTTGCTCCATGGTCATTCATTTGAGGTTTTAGAGTTTTGTAAGATA AATGAGAAGGCTATTATTTGTGCAGGAAATGTTCTTAAAAAGAAAATCTGGGGCACGGAGCCTCTATTTC TTGCCTACGTTTTTATTTCCATAAATCTTTTTTTTTTGGTTTTTGGTTTTTTTGTTTTGTTTTTTTCGAG ACAGGGTTTCTCTGTATAGCCCTGGCTGTCCTGGAACTCACTTTGTAGACCAGGCTGGCCTCGAACTCAG AAATCTGCCTGCCTCTGCCTCCCAAGTGCTGGGATTAAAGGCATGCACCACCACGCCTGGCTAATTTCCA TAAATCTTTGAACATAATTTGACACTGATGGCTTCTTTTAAAGTCCTTATTTCCAAATAGATCCTTCTAG TTCAAAGTACATATTAGGATTGATGGACGTGTCATTTACCACAGATCTAAGCAGAGTAAGTGTCAGACTC AGGCCGTGTCAGCAAACCTACACTGAGATTTCAACACAGCCTCTTGCAGTGTGCCTGTGTCTCTTACCTG GTGCGTCATAGGCGGGGCCGCCGAACTTGCCATCTATTTCCCTTTTGGGTGCTCACCCTTGTGGCCATTT TCAGGGGAGTTGACTGATCATGACTCGGGAATCTGTGTTGCCTTTGCTCACTGTGTCCTGATGATCACAA GCATCTTGGTTTCTCTGCTTTTCTCAGCTGGGAAGCTTCATGGCCACGCTGCTTTGTGGAGCTCGGGTTC TGTTTTACTTAAGTTAATGATTGCATGGGGGAGTGTTTGGGTCTTTAAAGTAATGTATAAAGACCAGAAT CTTGGCACTGTGCCTGTTCCACCTAACAAGGCATTCTTGGTGCTGGCTTTCGTGGGAGAGCAGGGTGGCG CTCTGCATTTCAGCGTGAGCTAGCTCTCACCCTGAAACCAATGTGCGACTCCAGAAGGTGACTGCTCGCA CTGTGCCTTTCCAGGCATTAAAGCGCTGTGACTGATTTTGCTTGGGAAGTGCCCAAAGAGGCAAGGCTAG AGAGCAGACTGGGAGGCAGCATGGTGGGATTCCACTGAAGCAAGAGCTGTGCAGCAGGATGGGAGCGTGG GCTTGGGGCATCCTCCAGGCGACTCTGGCTGGACCCATAAAGTCAAATTGTGAAAGGCCTCTTTCCACAC ATGGGCTGTGACTTAAAGCACTCGCTTAGTAGTAAGCTCTGAGAGGTGTTCGTCCTCAGTGCAGAAGCTA GGTGATCAGAGAACTGCAAGCATCCTTGGGGCCGGCTCAATTCAACAGGAGTTCTTCATGGGGGTTCTCT TCCCAGCAGGCCTGTGGTAATTGTGAAGATGTTTTTTTCCTATCATAACTTAAGGTGTGTGTGGCGGGGG TTGAGGTAGTGGTGTGTCATCATCTAGTGATAGATGTCCCGATGGCTGGCACAGCTAACCATCTGCCCCA AGGCAGTGATGCTGTGGTGGAGAAGCCGAGGGGAGAGCAGACCAGCAGCATAATGTGGTGTGGCCATTAC AGCTCTCCCAGCCTGTGTGGGAAATGCTGTTTGTCTTGGTTGTAGTTACCACTTGTCCACATACCCCACT GTCAGTCTTCATAAGACAAACTGCAATGTATGAACTTATTATATAAAACAATCCACAAATAAAAAGGAAA TGTTAATTGTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156934 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156934 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004315 same experiment
	EMAGE:29626 same embryo
	EMAGE:29857 same embryo
	EMAGE:31043 same embryo
	EMAGE:29894 same embryo
	EMAGE:32218 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source