EMAGE mouse gene expression spatial database

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EMAGE:29983

B830009D23Rik ( MGI:2139461)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_006245_06	euxassay_006245_07	euxassay_006245_08	euxassay_006245_09	euxassay_006245_10

euxassay_006245_11	euxassay_006245_12	euxassay_006245_13	euxassay_006245_14	euxassay_006245_15

euxassay_006245_16	euxassay_006245_17	euxassay_006245_18	euxassay_006245_19	euxassay_006245_20

euxassay_006245_21	euxassay_006245_22	euxassay_006245_23	euxassay_006245_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8544
Entity Detected:	B830009D23Rik, ( MGI:2139461)
Sequence:	sense strand is shown >T8544 GCTTTACAGGTGCTGAGGTCGCACCACGGTCAGGGATGCCAGACCCTGGTGGAGCCAGGTGGCGAGCTGC CTCCCTGCCACCCCCTCGCTTGGCTCTTCCTGGTCCTCACTAGGTTGTCAAACGCCGGGCAACCGGGCTG CCGCGGCGCGGAGGCCTGGGCTGAGGCGAGTTGCGGAGGCGGCGGGAGGCCACAGAGGCCGGAAGTGCGG GCCGGAGCGCTCCGGGGCTGCGCCGAGATGGCGAGAATGCTGGCGGTCCGGGTGGTGGCAGGGCTGGCGG CGGCCGCGGTGGCCGCCCTGCTGTTGGAGCACTACGGCCTGGCTGGGCCGACTACGCCGCTGCCGAAGCC CAGAGGCTCCCAGAGGCCGCACCCCGCCCCGGGCTCGGAAGCCAACAACATCTTCTGGGGGCTGCAGATA TCCGACATTCACCTGAGCAGATTTCAAGATCCAGGCAGAGCGCTGGCCTTAGAGAAGTTCTGTTCGGAAA CTATTGACATCATTCAACCGGCTCTTGTCCTGGCCACTGGAGACCTGACAGATGCCAAAACAAAGGAACA CTTGGGATCCAGACAACATGAAGTAGAATGGCAAACTTACCAGAGAATTCTGAAAAAGACAAGAGTCATG GAAAAAACCAAGTGGCTGGATATCAAAGGAAATCATGATGCATATAACATCCCAAGTCTGGAGAGCATCG CCAATTATTACAGGAAGTACTCTGCTGTGCGGAAGGACGGCGCTTTTCATCACATCCACAGCACTCCCTT TGGCAACTATTCCTTCATCTCTGTGGATGCCACCCAAAGGCCAGGGCCTAAGAGACCCTATAATTTCTTT GGCATTTTAGATGAGAAACAGATGGAGGAACTCGTAGAGTTTTCCAAGAAAAGCAGTCAGAGCAACCAGA CGATTTGGTTTGGCCACTTTACAACATCAACTATCATGTCTCCATCCCCAGGGATCCGGACAGTAATGGG TTCCGCTACAGCTTATCTGTGTGGGCACCTCCATACGCTTGGTGGGCTGATGCCTGTTCTGCACACCCGT CACTTCACAGGCACGCTGGAACTTGAGGTGGGAGACTGGAAAGACAACAGAAGGTACCGGATCTTTGCCT TTGATCACGACCTCTTTAGCTTTGCAGATGTAACCTTTGACAAGTGGCCTGTGGTTCTCATCACCAATCC CAAATCCCTCCTCTATAGCTGTGCTAAGCATGAGCCGCTAGAAAGGCTCTTCCATTCCACACACATAAGA GTATTGGCCTTTTCCTTATCCCCCATCACTTCTGTCACTGTTAAGATTGATGGAGGTGATATAGGCCAGG CTTCTCACTTGTCTGGTCCTATTTTCATACTGAAGTGGAACCCTAGAAACTACAGTAATGGGACACATAC CATAGAAGTATTCGTCCAGGATTCTGCTGGAAGAAGTAGGACTGCTCACCACATATTTTCTGCCCAAGAG GATGCTCATCTCACATTTGATCCTTTGGCATCATTTATCCTCCTTACTGACCACTACATTGTGGCCCGGG TCCTGTTTGTGTTGATTGTGCTGATCCAGCTCACCACACTGATTACATTCAGATATCTAGCATACCCAGA GCTTAAAGAACCTCTGGGATTTGCAAATATGACCACATTTTCCCTTCATATCTTGAGCAAAATAAACATC TCCTACTATTCTGTTTTGTTGTTGACCTTGTATACAGTGCTGGGACCATGGTTTGTTGGTGAGATAATTG ATGGCAAATTGGGTTGCTGCTTTTCATTTGGGATATTTGTTGATGGACATTTCCTGCAAGGTGGTCTAAC ATTTATAAATGGAATTATACAGCTGGTGTTCTTTAACATCCCCTTGATGGCTTACGTATGCTGGAGTTTG CTACATCGCTGCTTTGGTCACAGCTTCAGGTCTCATCTCCACCAGGGAAAATACTGGAAGATTATACCTG TTTACCTACTTATACTTCTACTGTACATATGGCAGGTTTATTCTTGCTACTTTCTTCATGTGACATATGG CTCCCTAGCCTTTACATTCTCTCCATTGAGGACCTGGTTGACATTGCTGACACCTGTGCTCATTTACCGT GTGTGGACATTGAACTCCACTGAACTTGGAATATTCATGGTGCAGTTAAAAAGTCATCTGAGCTCCTGAA GTCCATGGCTTACAGCTAGCAGCCTGGAAGAAGTCCAGCCTCCACATTTGTGGCTCAGATCTCTGCCCTG TGGCAGATGGGAAAGCCAGTATCAGTGGGTGAGCTTCAGGAAGCAGTCACTCCTTGGACATCTGAATGTT GGAAATGTTTACTACTGATTCTTGCTGTGTGGACTGCAGAAAAGTCTAACAATTGATTCTTCCTCAGGAG GCAAAGAGCCCTAATCAAGAAGATTGCCACAGTGGGGTCTCTGTGTCACTGTGTCCCTTGGTACTACTGT GTTTATTAGGGTCATCCTGTGAAAAGCATCCAGCCCGAACTTGGAGTTCAGGATGTCATTTTCTGCCTAG GGAGCCTGTGGAAACTTGTCCTGGACCTTTACTGCTCCTGAGACTTGACTTCCCAGCCTGTTTCCCCACC GATCATGGGCTTTCTGCTTCCTTAGCCAAGGTTGTTTTCAACTAATCAAATAAATGAATGAATGGTAGCC ATAAACAATGGGTTGGAGAGATCAGTGGTTAAGAGCATGTACTTTACCTCCAGAAGACCCGAGTTCAATC CCTAGCAACCATGTCAGATGCTCACAACTGCCTGTAACTCTAGCTCCAGGCCAGAGCCACTGTGGGCACC TACATTCATATGTGCACAGACTTACATGTGATTAAAAAATAATAAAAGATAAGTATTAACAAAGAAAAAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5708443 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5708443 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006245 same experiment
	EMAGE:29498 same embryo
	EMAGE:29974 same embryo
	EMAGE:29977 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source