EMAGE mouse gene expression spatial database

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EMAGE:30002

Zfp513 zinc finger protein 513 ( MGI:2141255)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_008434_11	euxassay_008434_12	euxassay_008434_13	euxassay_008434_14	euxassay_008434_15

euxassay_008434_16	euxassay_008434_17	euxassay_008434_18	euxassay_008434_19	euxassay_008434_20

euxassay_008434_21	euxassay_008434_22	euxassay_008434_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8389
Entity Detected:	Zfp513, zinc finger protein 513 ( MGI:2141255)
Sequence:	sense strand is shown >T8389 GGAAGCGGCGGCGGCGGCGGGAGCGACCTGAGCTGGATTCCGGGGCCGGGGCCAGGGGCTGCCCAGGGCC CGCACCGGTGTATGGGGGCGGTTCGTGGATCCTAAGAGCAAGGACCGACGGCAGGGCCGAACTGGGAGGG GCGGGGCCGGGAGGCCTCGGGCCGGAGGCGCGTCGGGCTGGAGCCGGTCACGATGCCCCGAAGGAAGCAA AGCCATCCACAGCCCGTGAAATGCGAGGGGGTCAAAGTGGATACTGAAGATTCCTTCGACGAAGGTCCTG GGGCCCTGGTGTTGGAGAGCGATTTGCTACTAGGCCAAGATCTGGAGTTTGAAGAGGAAGAGGAAGAGGA TGAAGGTGACGGCCACAACGACCAGCTCATGGGCTTTGAGAGAGACTCTGAAGGAGACTCTCAGGGGGCC AGACCTGGACTTCCCTATGGGCTGAGTGACGACGAGTCTGGGGGCGGCCGCGCACTAAGTGCGGAGAGTG AAGTTGAGGAACCAGCCAGGGGTCCAGGGGAGGCCAGGGGTGAGAGGCCAGGCCCAGCCTGTCAGCTGTG TGGGGGGCCGACAGGTGAGGGGCCGTGTTGTGGGGCAGGAGGGCCGGGTGGGGGGCCCCCGCTGCCCCCA CGGCTACTGTACTCATGCCGGCTGTGCGCCTTCGTGTCCCACTACTCGAGCCACCTGAAGCGGCACATGC AGACACACAGCGGGGAGAAGCCGTTCCGCTGTGGCCGCTGCCCCTACGCCTCAGCCCAGCTCGTCAACCT GACGCGACATACCCGCACCCATACTGGCGAGAAGCCCTACCGCTGTCCCCACTGCCCCTTTGCCTGCAGC AGCCTGGGCAACCTGAGGCGGCATCAGCGCACCCACACAGGGCCTCCCACTCCTCCCTGCCCAACCTGTG GCTTTCGATGCTGTGCTCCACGACCAACCCGGCCTCCCAGTCCCACAGAGCAGGAGGGGACAATGCCCCG ACGATCAGAAGATGCGCTGATCCTGCCAGACTTGAGTCTTCATGTGCCACCAGGTGGTGCCAGTTTCCTG CCAGACTGTGGGCAGCTGCGGGGTGAAGGGGAGAGCTTGTGTGGAACTGGATCCGAACCACTGCCAGAGC TACTGTTCCCTTGGACCTGCCGGGGCTGTGGACAGGAACTGGAGGAGGGTGAGGGCAGCAGGCTGGGAGC TGCCATGTGTGGGCGCTGCATGCGAGGAGAGGCTGGAGGGGTTGCCACTGGGGGACCCCAGGGCCCTGGT GACAAAGGCTTTGCCTGTAGTTTATGCCCCTTTGCCACTCACTACCCCAACCACCTGGCTCGGCACATGA AGACTCACAGTGGTGAGAAACCCTTCCGCTGTGCCCGCTGTCCATACGCCTCTGCTCATCTGGATAACCT GAAACGGCACCAGCGCGTCCACACAGGAGAAAAGCCCTACAAGTGCCCCCTCTGTCCGTATGCCTGTGGC AACCTGGCCAACCTCAAGCGTCATGGTCGCATCCACTCTGGTGACAAACCTTTTCGGTGTAGCCTTTGCA ACTACAGCTGCAACCAGAGTATGAACCTCAAACGTCATATGCTGCGACACACGGGCGAGAAGCCCTTCCG CTGTGCCACCTGCGCCTATACCACAGGCCACTGGGACAACTACAAGCGTCATCAGAAGGTGCATGGCCAT GGTGGAGCAGGAGGGCCTGGTCTCTCTGCCCCTGAGGGCTGGGCCCCACCTCATAGCCCACCCTCTGTTT TGAGCACTCGGGGTCCAGCAGCCCTGGGTGCTACTGGTAGCAGGGCTCTTCATTCAGACTCACCTTGAAC TAACTAGGTTCTTTTACCTGGGGCTCTAGGAATTAGCCCTATGCCTCCTGCATTTTATACAAATGAACTA GAAACCACCTTTCCCTTTCTCCCCCGCTGGTCAGGGGCTCCACACAGACTAACCTAGGCACTATATGGAC CAGCCTGAATCCCATGGTCAGGGGGCCATATAGACCAGGGGACTTGTCTTAGCTCACGTACCAGATGAGC TAAGTGATTAGGGCCTTGGATTCACCGCCACTGCTCCCAGAGGCTATGGATGAACTGGTTGGGAGCTGCC CAGCCTTTTACTGTTTTAACTTATTTCAGTGCTTTATAATAAAGGAAACACTAACAGAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5685033 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5685033 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008434 same experiment
	EMAGE:29956 same embryo
	EMAGE:30848 same embryo
	EMAGE:30144 same embryo
	EMAGE:29955 same embryo
	EMAGE:29965 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source