EMAGE mouse gene expression spatial database

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EMAGE:30033

Carm1 coactivator-associated arginine methyltransferase 1 ( MGI:1913208)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005357_01	euxassay_005357_02	euxassay_005357_03	euxassay_005357_04	euxassay_005357_05

euxassay_005357_06	euxassay_005357_07	euxassay_005357_08	euxassay_005357_09	euxassay_005357_10

euxassay_005357_11	euxassay_005357_12	euxassay_005357_13	euxassay_005357_14	euxassay_005357_15

euxassay_005357_16	euxassay_005357_17	euxassay_005357_18	euxassay_005357_19	euxassay_005357_20

euxassay_005357_21	euxassay_005357_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4452
Entity Detected:	Carm1, coactivator-associated arginine methyltransferase 1 ( MGI:1913208)
Sequence:	sense strand is shown >T4452 CCACGCGTCCGGGCGGCGGCGGCGGCGGCGGCGGCCTGGGCCCGGGTGCGGCGGTGGCGGTGGCCGTGGT GGCAGCGGGGCCTGGAGCCGGACCTAAGATGGCAGCGGCGGCAGCGACGGCGGTGGGGCCGGGTGCGGGG AGCGCTGGGGTGGCGGGCCCGGGCGGCGCGGGGCCCTGCGCTACAGTGTCTGTGTTCCCGGGCGCCCGCC TCCTCACTATCGGCGACGCGAACGGCGAGATCCAGCGGCACGCGGAGCAGCAGGCGCTGCGCCTTGAGGT GCGCGCCGGACCAGACGCGGCGGGCATCGCCCTCTACAGCCATGAAGATGTGTGTGTTTTCAAGTGCTCG GTGTCCCGAGAGACAGAGTGCAGTCGTGTGGGCAGACAGTCCTTCATCATCACCCTGGGCTGCAACAGCG TCCTCATCCAGTTTGCCACACCCCACGATTTCTGTTCTTTCTACAACATCCTGAAAACCTGTCGGGGCCA CACACTGGAGCGCTCTGTGTTCAGTGAGCGGACAGAGGAATCCTCAGCTGTGCAGTACTTCCAGTTCTAT GGCTACCTATCCCAGCAGCAGAACATGATGCAGGACTATGTGCGGACAGGCACCTACCAGCGTGCGATCC TGCAGAACCACACGGACTTCAAGGACAAGATCGTTCTAGATGTGGGCTGTGGCTCTGGGATCCTGTCATT TTTTGCTGCTCAAGCAGGAGCCAGGAAAATTTATGCAGTGGAAGCCAGCACCATGGCTCAGCATGCAGAG GTCCTGGTGAAGAGTAACAATCTGACAGACCGCATCGTGGTCATCCCTGGCAAAGTAGAGGAGGTCTCAT TGCCTGAGCAAGTGGACATTATCATCTCAGAGCCCATGGGCTACATGCTCTTCAATGAACGAATGCTCGA GAGCTACCTCCATGCCAAAAAGTACCTGAAGCCTAGTGGAAACATGTTCCCCACCATTGGTGATGTCCAC CTCGCACCCTTCACTGATGAACAGCTCTACATGGAGCAGTTCACCAAAGCCAACTTCTGGTACCAGCCAT CCTTCCATGGAGTGGACCTGTCGGCCCTCAGAGGTGCCGCTGTGGATGAGTACTTCCGGCAACCTGTGGT GGACACATTTGACATCCGGATCCTGATGGCCAAATCTGTCAAGTACACAGTGAACTTCTTAGAAGCCAAA GAAGGCGATTTGCACAGGATAGAAATCCCATTCAAATTCCACATGCTGCATTCAGGGCTAGTCCATGGCT TGGCCTTCTGGTTCGATGTTGCTTTCATTGGCTCCATAATGACCGTGTGGCTATCCACAGCCCCAACAGA GCCCCTGACCCACTGGTACCAGGTCCGGTGCCTCTTCCAGTCACCGTTGTTTGCCAAGGCCGGGGACACG CTCTCAGGGACATGTCTGCTTATTGCCAACAAAAGACAGAGCTATGACATCAGTATTGTGGCACAGGTGG ACCAGACAGGCTCCAAGTCCAGTAACCTGCTGGATCTAAAGAACCCCTTCTTCAGGTACACAGGTACAAC CCCATCACCCCCACCTGGCTCACACTACACGTCTCCCTCGGAGAATATGTGGAACACAGGAAGCACCTAT AATCTCAGCAGCGGGGTGGCTGTGGCTGGAATGCCTACTGCCTACGACCTGAGCAGTGTTATTGCCGGCG GCTCCAGTGTGGGTCACAACAACCTGATTCCCTTAGGCTCCTCAGGTGCCCAGGGAGGCGGCGGTAGCTC CAGTGCCCACTATGCAGTCAACAACCAGTTCACCATGGGTGGCCCTGCCATCTCTATGGCCTCGCCCATG TCCATCCCGACCAACACCATGCACTATGGGAGTTAGGTGCCTCCAGCCGCGACAGCACTGCGCACTGACA GCACCAGGAAACCAAATCAAGTCCAGGCCCGGCACAGCCAGTGGCTGTTCCCCCTTGTTCTGGAGAAGTG TGAACACCCGGTCACAGCCCTCTTTGCTATGGGAACTTGGACAATTTTGTACACGATGTCGCCGCTGCCC TCAAGTACCCCCAGCCCAACCTTTGGTCCCGAGCGCGTGTTGCTGCCATACTTTACATGAGATCCTGTTG GGGCAGCCCTCATCCTGTTCTGTACTCTCCACTCTGACCTGGCTTTGACATCTGCTGGAAGAGGCAAGTC CTCCCCCAACCCCCACAGCTGCACCTGACCAGGCAGGAGGAGGCCAGCAGCTGCCACCACAGACCTGGCA GCACCCACCCCACAACCCGTCCTTGCACCTCCCCTCACCTGGGGTGGCAGCACAGCCAGCTGGACCTCTC CTTCAACTACCAGGCCACATGGTCACCATGGGCGTGACATGCTGCTTTTTTTAATTTTATTTTTTTACGA AAAGAACCAGTGTCAACCCACAGACCCTCTGAGAAACCCGGCTGGCCGGGCCAAGCCAGCAGCCCCTGTT CCTAGGCCCAGAGGTTCTAGGTGAGGGGTGGCCCTGTCAAGCCTTCAGAGTGGGCACAGCCCCCTCCCAC CAAAGGGTTCACCTCAAACTTGAATGTACAAACCACCCAGCTGTCCAAAGGCCTAGTCCCTACTTTCTGC TACTGTCCTGTCCTGAGCCCTGAAGGCCCCCCTCCATCAAAAGCTTGAACAGGCAGCCCAGAGTGTGTCA CCCTGGGCTACTGGGGCAGACAAGAAACCTCAAAGATCTGTCACACACACACAAGGAAGGCGTCCTCTCC TGATAGCTAACATAGGCCTGTGTGTTGCGTTCACATTCATGTTCTACTTAATCCTCTCAAGACAGCAACC CTGGGAAGGAGCCTCGCAGGGACCTCCCCAGACAAGAAGAAAAGCAAAGAAGGAAGGGTGATTAATAAGC ACAGGCAGTTTCCCCTATTCCCTTACCCTAGAGTCCCCACCTCAATGGCCACAGCCTGCCACAGGAACCC CTTGGCAAAGGCTGGAGCTGCTCTGTGCCACCCTCCTGACCTGTCAGGGAATCAGAGGGCCCTCAGGCAG CTGGGAACCAGGCTCTCTCCTGTCCATCAGTAATACTCCTTGCTCGGCTGCCCCTCCCCCACCTTTATAT AAATTCTCTGGATCACCTTTGCATAGAAAATAAAAGTGTTTGCTTTGTAAGAAAAGCCTGCAAAACAGCA GGGTAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4935077 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4935077 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005357 same experiment
	EMAGE:30009 same embryo
	EMAGE:30020 same embryo
	EMAGE:30036 same embryo
	EMAGE:30795 same embryo
	EMAGE:30881 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source