Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30132

4930562D19Rik ( MGI:3041210)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132
euxassay_013850_01 euxassay_013850_02 euxassay_013850_03 euxassay_013850_04 euxassay_013850_05
EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132
euxassay_013850_06 euxassay_013850_07 euxassay_013850_08 euxassay_013850_09 euxassay_013850_10
EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132
euxassay_013850_11 euxassay_013850_12 euxassay_013850_13 euxassay_013850_14 euxassay_013850_15
EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132 EMAGE:30132
euxassay_013850_16 euxassay_013850_17 euxassay_013850_18 euxassay_013850_19 euxassay_013850_20
EMAGE:30132
euxassay_013850_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T38269
Entity Detected:4930562D19Rik, ( MGI:3041210)
Sequence:sense strand is shown

>T38269
TATGACTGCCAAGTTCATCAGGCCCCCTTCCCCGGTGCCAGGCTTGCCTTTGATCTGCCAAGGGTTGCAG
AGCATCCCTGTCAGAGTCTCCCTGCGGAGCCCAGGTGGAACCTCTGAGAAAACTAAGAGCGTCTACTCCC
AGACCGTCGAGACAGCCTTGGTGCCCTGTGATGCTTGCACCAGTGTCCAGGGCAGCTTGTGGGAGGTGGG
CAAGGTTGTTATCAGCCTGTGTCAGAGCCAAAACTTGCCTTCATCCTTGGGCCAGTTCCAGAAGTTGGTG
AAGGACAGCCTGGGGCTTAAACCACTGCCAGCTGCCACCGTGGGCCACTGGGCAGCAGAGCAGAGCAAAG
ACCTGACTCGTCTCAATAAACATGTGGGGGCCCTCACCCAGCTTGTTGGGCCCCTCAGGGCTCAGCTGGA
AGACGCTGAGGGGCAGAAGGATGGGCTGAGGAAGCAGGTGAGCAAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 142895. Forward Primer - name:142895_F_cDNA_4930562D19Rik, sequence:TATGACTGCCAAGTTCATCAGG; Reverse Primer - name:142895_N_SP6_cDNA_4930562D19Rik, sequence:CTTGCTCACCTGCTTCCTCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_013850 same experiment
 EMAGE:30814 same embryo
 EMAGE:31814 same embryo
 EMAGE:30135 same embryo
 EMAGE:31145 same embryo
 EMAGE:30166 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS