EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30137

Nipa2 ( MGI:1913918)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_013857_01	euxassay_013857_02	euxassay_013857_03	euxassay_013857_04	euxassay_013857_05

euxassay_013857_06	euxassay_013857_07	euxassay_013857_08	euxassay_013857_09	euxassay_013857_10

euxassay_013857_11	euxassay_013857_12	euxassay_013857_13	euxassay_013857_14	euxassay_013857_15

euxassay_013857_16	euxassay_013857_17	euxassay_013857_18	euxassay_013857_19	euxassay_013857_20

euxassay_013857_21	euxassay_013857_22	euxassay_013857_23	euxassay_013857_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38239
Entity Detected:	Nipa2, ( MGI:1913918)
Sequence:	sense strand is shown >T38239 TCAACACTTCCATTGTGACTCCAATATATTATGTGTTCTTTACAACTTCAGTTTTGACTTGTTCAGCTAT TCTTTTTAAAGAATGGCAGGATATGCCTGTTGATGATGTCATTGGGACTTTGAGTGGCTTCTTCACAATC ATTGTGGGGATATTTTTGTTGCATGCCTTTAAAGATGTCAGTTTTAGTCTAGCAAGTCTGCCAGTATCTT TTCGAAAAGATGAAAAGGCAATGAATGGCAATCTTTCTAGTATGTATGAAGTTCTTAATAATAATGAAGA CGACTTACCCTGTGGAATTGAACACACTGGTGAAAATATTTCCCGTAGGAATGGAAATCTGCCATCTTTT TAAGGACATGTTTGAAAAGTTAACCTATAATTCTGTTATAAAGTGAGTTTTGAGCATCAGAATGTATCTG AAAAAATTATCCTCAAATAATGTCCTCTAAAGACAATCTTTTTTTAAGTTTTCACTAATTTGGATCAAGA AATTACTTTTCTTATAGTTAAACAATGGTAGCTCACTAATATGACCTCAGTACATGGCTAATTTCTAATA ACATTGTATTATTGTAAAGGTATTTTACATTTTTATTCATTCTCCAGAAATTTAAATGCACTAATGACAA GTTTTAAGCTTATAAAAATGTTTTATTTTTTCCATTGGTGATGAACATTAAAATGTACATTTGTGATCCC CATTCCATCAATACCTCATCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 145156. Forward Primer - name:145156_F_cDNA_3830408P04Rik, sequence:TCAACACTTCCATTGTGACTCC; Reverse Primer - name:145156_N_SP6_cDNA_3830408P04Rik, sequence:TGATGAGGTATTGATGGAATGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_013857 same experiment
	EMAGE:31031 same embryo
	EMAGE:30969 same embryo
	EMAGE:30124 same embryo
	EMAGE:30101 same embryo
	EMAGE:30970 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source