EMAGE mouse gene expression spatial database

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EMAGE:30211

Chchd6 ( MGI:1913348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002687_01	euxassay_002687_02	euxassay_002687_03	euxassay_002687_04	euxassay_002687_05

euxassay_002687_06	euxassay_002687_07	euxassay_002687_08	euxassay_002687_09	euxassay_002687_10

euxassay_002687_11	euxassay_002687_12	euxassay_002687_13	euxassay_002687_14	euxassay_002687_15

euxassay_002687_16	euxassay_002687_17	euxassay_002687_18	euxassay_002687_19	euxassay_002687_20

euxassay_002687_21	euxassay_002687_22	euxassay_002687_23	euxassay_002687_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5833
Entity Detected:	Chchd6, ( MGI:1913348)
Sequence:	sense strand is shown >T5833 CGCTGAGCAGCGTCGTCCTGGTTGCTGTGGAGCCGGCTCGCGTGGCCTCACGCCCTGCCCGCCTCCTGTC ATGGGCAGCGCCGAGAGCGCCGAGGCCCGAAGGGTGTCCTTCGAGATGGATGAGGAGGAGCGGGTCCGGG TGCTGCAGGGCATCCGGCTGTCTGAAAGTGTTGTGAACCGCATGAAGGATTGCAGCCAGCCTTCAGCAGG GGAACAGCTGGTTCCTGGTTTTGGTCCTTCGTCTTCTGCTCCTGTGCCCACTGTCCCTCTACCAGCCATC TCTGTGCCCACTGTCCCTGCACCTACCACTCCAGTGCCTACTGCTCCTTCATCCTCTGTACGTGGCCTGC CAGGAGGCACCTGTAAAGGCCCTCTGACAGACGTTAAAGTGCCCAGTGCAGAGAGTGGTGGTGGTCTGCA GTCCTCAGCAGTGAAGGAGGATCTTAAGAAGTTCCAACAGGAGCAGTTGGCAGTCCAGGATGAGATGGTC AGGGTGGCAAAAAAGGAAAAAGAGGCAGCTGAGAAGCACTTGAAGGCATCCCTGCCCAAGAAGAAGGCCA GCCTCACCCATGAGCAACAGCAGTCAGCTCGACTAGCCAGGGAACTGGAGGACAGAGAGGCCGAGCTCAG CCGCTGTGACACCTTCTACAAGGAGCAGCAGGGGCGCATCCAGGAAAAGAATGCTGAGCTGTATAAACTG TCTTCCCAACAATTCCATGAGGCGGCTTCAAAAGCAGAGAGCACAATAAAGCCCCGCAGAGTGGAGCCTG TGTGCTCTGGCCTACAAGCCCAGATCCTCCGCTGTTACCGGGACCATCTGCATGAAGTGCTTCTGTGCTC GGACCTGGTCAAGGCATACCAGCACTGTGTGAGCACTGCCCGCAAGGGCTGAGGAGCAGCATCCTTCCTG GAGCCTTGAAGAAGGGACCGATCATAGGACCACAGACCATGGCCCCCAGGCCCTGCAGTCTCTAACTGAG CACCCATATGCCTCTGAGTGTGGGGCTGCCATGCTCGAGAAACAGTATGTGCTACTGATTGAAAACAAAT AAAGCAGATGTCTTTGTTTGCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3153325 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3153325 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002687 same experiment
	EMAGE:30142 same embryo
	EMAGE:31240 same embryo
	EMAGE:30234 same embryo
	EMAGE:31595 same embryo
	EMAGE:30226 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source