EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30213

Drap1 Dr1 associated protein 1 (negative cofactor 2 alpha) ( MGI:1913806)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002209_01	euxassay_002209_02	euxassay_002209_03	euxassay_002209_04	euxassay_002209_05

euxassay_002209_06	euxassay_002209_07	euxassay_002209_08	euxassay_002209_09	euxassay_002209_10

euxassay_002209_11	euxassay_002209_12	euxassay_002209_13	euxassay_002209_14	euxassay_002209_15

euxassay_002209_16	euxassay_002209_17	euxassay_002209_18	euxassay_002209_19	euxassay_002209_20

euxassay_002209_21	euxassay_002209_22	euxassay_002209_23	euxassay_002209_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6029
Entity Detected:	Drap1, Dr1 associated protein 1 (negative cofactor 2 alpha) ( MGI:1913806)
Sequence:	sense strand is shown >T6029 CCGAAGCCCAGCGCTCGAGTTTGGGGAGCTGCTTGTCTACGGGCGGCACTAGGACCCTGCGGGAAGTGGT GTAGGCGGAGTAAACGGCACCAGGACCCGGGGAGGGTGGGGGGGGGGACCGCGGCGGGCCGGCGGCGGAC AGGCCCGGGGCCGGGAGGCTGCGGGCGGCGGCGCTGGGCCCGGCGCAACGAGAGGCCCCGAGATGCCAAG CAAGAAGAAGAAGTATAACGCGCGGTTTCCGCCGGCGCGGATCAAAAAGATCATGCAGACGGACGAAGAG ATTGGGAAGGTGGCGGCAGCTGTGCCTGTCATCATCTCCCGGGCCCTTGAGCTCTTCCTGGAGTCCCTGT TGAAGAAGGCTTGCCAGGTGACCCAGTCTCGAAATGCCAAAACCATGACCACGTCCCACCTGAAGCAGTG CATTGAACTCGAGCAGCAGTTTGACTTCTTGAAAGACTTGGTGGCATCAGTGCCTGACATGCAGGGGGAT GGGGAGGACAACCACGTGGATGGGGACAAGGGTCCTCGAAGGGGCCGGAAACCGGGCAGCAGCGGCAGGA AGAACGGAGGCACGGGAAGCAAAGGCAAAGACAAGAAGCTGTCCGGGACAGACTCTGAGCAGGAGGATGA GTCTGAGGATACAGATACTGATGGGGAAGAAGAGACACCACAGCTTCCACCCCAAGCCAGTCACCCTCCT GCCCACTTTCAGAGCCCTCCAACTCCCTTCATACCCTTCACATCTCCCCTGCCTTTGCCACCAGCACCCC CCGGCCCCTCAGCAGCCGATGCAGAGGATGAAGAGGATTATGACTCCTAGCGCCCTGTGCCCCTGTACCC CAGCCATGCTCTCTTCTAGTTAGTATTAATTGCTTTGGGGAGAGGAGAAAGGTGGGATTGTTCTTAAATT TATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3488139 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3488139 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002209 same experiment
	EMAGE:29946 same embryo
	EMAGE:31658 same embryo
	EMAGE:30250 same embryo
	EMAGE:30232 same embryo
	EMAGE:31655 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source