Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30271

Efcab4b ( MGI:2685919)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271
euxassay_013790_01 euxassay_013790_02 euxassay_013790_03 euxassay_013790_04 euxassay_013790_05
EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271
euxassay_013790_06 euxassay_013790_07 euxassay_013790_08 euxassay_013790_09 euxassay_013790_10
EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271
euxassay_013790_11 euxassay_013790_12 euxassay_013790_13 euxassay_013790_14 euxassay_013790_15
EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271 EMAGE:30271
euxassay_013790_16 euxassay_013790_17 euxassay_013790_18 euxassay_013790_19 euxassay_013790_20
EMAGE:30271 EMAGE:30271 EMAGE:30271
euxassay_013790_21 euxassay_013790_22 euxassay_013790_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37869
Entity Detected:Efcab4b, ( MGI:2685919)
Sequence:sense strand is shown

>T37869
GGAATTCTTCCAGACCTGTGACTCCGAGGGCAAGGGCTTCATTGCCAGGACAGACATGCAGAGGCTGCAT
CAGGAGTTGCCCCTAAGTCTAGAAGAATTGGAAGATGTGTTTGACGCCCTGGATGCTGATGGCAATGGCT
TTCTGACCCCAGAAGAGTTCACCACTGGATTTAGTCACTTCTTCTTCAGTCAAAATATCCAAGGAGAGGA
AGAAGCTGACCAGCAGGTGGCCCAGCTCCAAGAGGAGAAAGTGTATCAGTCTAGAGGGGAGGAGGATGTG
GGAGACATGGACCATGATGAAGAAGCACAATTTCAGATGCTGATGGACAGACTTGGAGCCCAGAAAGTTC
TGGAAGATGAAAGTGACGTCAGGCAGCTGTGGCTGCAGCTGAGGAAGGACGAGCCCCACCTGCTGTCCAA
CTTCGAAGACCTCCTCACCACAATTTTTGCTCAGCTCCAAGAAGCCCATGAGCAGAAGAACGAACTGGAA
TGTGCCCTCAGAAAGAAAATTGCTGCTTACGATGAAGAAATCCAGCATCTCTATGAGGAGATGGAGCAGC
AGATCAAAAGTGAGAGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79853. Forward Primer - name:079853_F_cDNA_LOC381812, sequence:GGAATTCTTCCAGACCTGTGAC; Reverse Primer - name:079853_N_SP6_cDNA_LOC381812, sequence:CCTCTCACTTTTGATCTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_013790 same experiment
 EMAGE:29637 same embryo
 EMAGE:31308 same embryo
 EMAGE:29639 same embryo
 EMAGE:29642 same embryo
 EMAGE:30304 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS