EMAGE mouse gene expression spatial database

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EMAGE:30391

Tbc1d14 ( MGI:1098708)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006610_01	euxassay_006610_02	euxassay_006610_03	euxassay_006610_04	euxassay_006610_05

euxassay_006610_06	euxassay_006610_07	euxassay_006610_08	euxassay_006610_09	euxassay_006610_10

euxassay_006610_11	euxassay_006610_12	euxassay_006610_13	euxassay_006610_14	euxassay_006610_15

euxassay_006610_16	euxassay_006610_17	euxassay_006610_18	euxassay_006610_19	euxassay_006610_20

euxassay_006610_21	euxassay_006610_22	euxassay_006610_23	euxassay_006610_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7136
Entity Detected:	Tbc1d14, ( MGI:1098708)
Sequence:	sense strand is shown >T7136 CGCGGGCGGAGCCGACCAGTTTCTTCTGGGACCAAGATGACTGATGGAAACCTTTCCACCTCGATGAATG GTGTAGCATTGATGGGCATCCTAGATGGTCGGCAAGGAGACTCCCTTCAGGACCTACAACACCTTAGTAT CAAGGCGGCTCCCAGATCCCTTTCAGTGCCTGAATACGGACCTTCACTAAAACTTGGTGCTTTGGAAGAT CGACACAGCCTTCAATCAGTGGACTCGGGCATTCCTACCCTGGAGATTGGCAACCCAGAGCCTGTTCCCT GCAGTGTGGTCCATGTGAAGAGAAAGCAGTCTGAGTCAGAGATCGTCCCGGAGCGGGCCTTCCAGAGTGC ATGCCCCCTGCCGTCCTGCACACCCTCAGCTCCCACCTGCAGCGAGCGGGAGCAGGTTGTGCGGAAGTCT TCCACGTTTCCTAGGACAGGCTATGACTCAGTGAAACTCTACAGCCCCACCTCCAAAGCCTTGAGCCGAA GTGACAATGTCTCTGTCTGCAGTGTGTCTAGTCTTGGCACAGAGCTGTCAACTACGTTGTCGGTCAGCAA TGAGGACATCTTGGACCTCATGGTCACGAGCAATTCCAGCGCCATTGTGACCCTGGAGAATGATGATGAC CCACAGTTTACTGACGTCACCCTGAGCTCCATCAATGAAACCAGCGACTTACACCAGCAGGACTGTGTTG CTGAGACTGAGGAGGGGAGGAAATTGAAACTATTGCAACCATTCAGTCACTTCTTTACAAGGAATTTGCT TGCTAGAAAACAAAATGCAAGGCTTGATAGACAGAGAGACTTGGGATGGAAGCTGTTTGGAAAAGTCCCA CTCCGAGAGACTGCTCAGAAAGATTCAAAGAAGACCCAGAAGGAATATGAAGACAAGGCTGGAAGACCGA GCAGGCCACCCTCTCCCAAGCAGAATGTGAGGAAGAACCTGGATTTTGAGCCACTGTCCACCACGGCGCT CATCCTCGAGGACAGACCTGCAAATCTCCCAGCCAAGCCAGCTGAAGAAGCTCAAAAGCACAGACAGCAG TATGAAGAGATGGTGCTGCAGGCCAAGAAACGAGAGCTTAAAGAAGCCCAGCGTCGGAGGAAGCAGCTGG AGGAAAGGTGCAAGGTTGAAGAGAGCATTGGGAACGCTGTCCTCACCTGGAACAATGAGATCTTGCCTAA CTGGGAAACCATGTGGTGCTCTAAGAAGGTTCGAGACTTGTGGTGGCAGGGAATCCCTCCGAGTGTAAGA GGCAAAGTTTGGAGCTTGGCCATTGGCAACGAGTTGAACATCACTCACGAGCTCTTTGACATCTGTCTTG CTCGGGCCAAGGAGCGGTGGCGGTCCCTCAGCACTGGAGGCTCAGAAGTGGAGAATGAAGATGCTGGGTT TTCAGCAGCAGACAGAGAGGCCAGTCTGGAGCTTATTAAACTGGACATTTCTAGAACATTTCCTAATCTC TGCATTTTCCAGCAAGGTGGTCCGTATCATGACATGTTGCACAGTATTTTGGGCGCTTATACTTGTTACC GGCCGGATGTGGGTTATGTTCAGGGCATGTCCTTTATAGCTGCAGTGTTGATCTTGAACCTCGATACTGC AGATGCCTTTATTGCTTTTTCTAATCTTTTGAATAAACCCTGTCAAATGGCGTTTTTCCGAGTGGACCAT GGCCTTATGTTGACTTACTTTGCTGCATTTGAGGTATTCTTTGAAGAAAACTTGCCGAAATTGTTTGCGC ATTTCAAGAAAAACAACTTAACTGCAGATATCTACCTAATCGATTGGATTTTCACCTTGTACAGTAAGTC CCTGCCCTTGGACTTGGCCTGCCGCATCTGGGACGTATTCTGTCGAGATGGAGAGGAATTTCTCTTCCGC ACAGCTCTGGGCATCCTGAAGCTTTTTGAAGATATCCTGACCAGGATGGACTTCATCCACAGTGCCCAGT TTCTGACCAGGCTGCCTGAGGACCTGCCCGCAGATGAAGTGTTTGCAGCTATCTCCACAGTCCAGATGCA AAGCCGGAACAAGAAATGGGCACAGGTGCTGTCTGCACTGCAGAAAGACAGCCGCGAGATGGAGAAAGGA AGCCCATCCCTCAGACACTGAGGCTCAGGGTAACTCACTCCCACCGGTCAAGAAGCAGAGCTCAACATGG CACCTGTGTGCTTTCAGACTGAAGCCTGCCTGCCAAGAACAGGGGCTCTTTCCATGTTGGATTTCTGATG CTGAAGTTGGCCTTACAACAAAACAGAATTACATCCAGGTTTAGCCTTAAGCAGCTCAGTGGGCCCATGC ACTGGCATTGGTCATGGCTAGCCACTGCATTTTCTACATGGGTTCAAGAAAGGGCAGGCTGTCTCCTTGA ACCTTTATTCCAGCAGCAGTTAATTAAATTGTGATGCTATTATGCCAACTACTGGAAGGTACATTACAAT CTTTCTTAGCCAGGATGCATGAGAAATGTTATTAGGAAACCTCAGAGTTGTTTTTGGCCCATATGCTTGT AAGAAAGGAATTACTTAGTTACTGAGATAATCTTTCCAGAGATGAAAGGTGTGTTTTAAATCTCCCTATC AAGGACATTGTAAGGAAGATGGGCCCTTGCAGGGTAGTTGCTGGTGAATCTCATAGGCTCCTGGGAACGC ACCTGACTTTTGTGCTGACATTGACCCATTGTTGCCTTGACACTTGATAGCAAGAAGGCTGAGAGTTCAT ATCAGGATCCTACTCTGCAAAGGAGCACTCATTGCATCTTGGGAGCTAGAGTGGTTGGAGCAGGGAAGCC ATATGCTGTGAAGGGACAGAGACAGGCCCTGTAAAGAGATAGGAAGGACGAGACTGCAGAGTTTGCTCCT CTTTAACCTGCTTGCTTCTGGGGAGGCTGGGCATGGCAGCCTCTGAAAACACCATTGTGTAGGAGATGGC TTCCTTGCTTTAGCTGAATGTCCCTTTTTGCCTTTGTCACCTGGGTAGCTGGATCCCTTCTAAGTGCCAG TGATTCTCCCCTGTCCCCGTCTGGCTTTAGAAATGAGTTCCACGCACTGCTATAAGGACAAGAGTAAGGC AGCCTTCCACCCTCTGTGTCCTGAGCCCTTCCCCATTGCAGCTGATTCCTCTCAGTTCATTGGTGAGGTG CTTGTTGCCCTAGCTTTTCCTTGACTTGAGAACTTTGTATAAGAAATTTTATTATCCAGATTCACAGAAA TGAACAGTTTCTACGTAGAGTAGAACAGTCCAGTATCTGTGGAAACTGGTTACCTTCTGATATCCATTGG CATTCTGGTTCCTGAGGGTGCAGGAAGGCCAGGGTTGAGAATGTATTTTCCAGCAAATCCCTGTGTGGTC TGTAGTCCACAGCCCTCCACAGCCGTACCTCCCACCCCCAGAACTCACCCTTTCCATCTCTGTGTTTCTG AGAGCTGTGGGGGAGCCCTTGACTGACTACAGCATGTGGCAGGGAGGCTTGGGGTCAGCCTCATGGCGCT GTGTTTGCATGGGTTCCCTTCATCTGCTATGCCTGTTGGTGACCGTCCAGTGTATCTGCAGGCTGTCGTA TTTGTGCTCTTCACCCCATGCTCTCACTTTCCTCTTTTCTTGGAGTCTCATTTGTAGTCTGTGACTTCTT CAGGGAGGCCACAGAGCACCCTCCATGGAGCTCTCTGCCTTAGTGTGAAAGGCTGATGTCTGACCTCTTG GAGGAGGATGTCTTCACAGCCATCCATGGCCTCCAAACCCAGCCATTCCTGACATCAGACACTGAATGTA GCCCAGGCCCAGCTGGCCATAAGATGCGCACTACCCAGCCCTGTCTGCTGTCCTTCCTTGCAGATGTGTA CTGAGTTTTCTCCCACAACATGGGCATCTGTTCTCTCAAGATGTATATATTTTCTTACTGTACATAAAGA CATTCCTTACGTCATACAGAAAGTGTTGTGACAAGGGGACTTGGGCACCTTGTTGTGAATGCCATTGTGG GATTCTCTGTGTTCACAACCCAGCCCTTACCTTTTCTTGTTTGACATAAATCTGTGGGCATGCATGAAGT TCTCCAACAAGACTCCATGCCAGCCGAGCACTGACTGCATCTCTAGTCCAAACAAGGTCTTTATAGACTG ACTGGGACTTGTGTGAGCCTGATGTACCCTCCTTCGAGGCTATACAGTTCCACGTTCCTCTACTTTGAGG GCGTCTCTCATACTTCTCTCATACTCCTGTTTTGGCAAACTGGAAACTGACCCTGCAGAGGTCTGTACCT CCCACCTCAATTGCCATTGACTTTCAGTCCCAAACTTGAGGTTGCTGGGCAGCTGGCACTCTGGAGGGAG CTTGCAGGAGAGTTCCTGTCACCTTGGGAGCAGTGCTTACTTCAGAGGTACTTCTAACTGCTCAGTTTGC CAATAGGAAACTCCTGATAACACTTGCTACATATCATGTTTTAATTGCTTGTACAGTTAACCTTTAATTT TGTTTAGTAAAGTGTATCAAACTAGGACTTTTTGAATTGTAAATATGTGGTTTTATTAAAATAAAGTCAT GTAAAATTGTTACTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4020510 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4020510 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006610 same experiment
	EMAGE:30329 same embryo
	EMAGE:30318 same embryo
	EMAGE:30921 same embryo
	EMAGE:30948 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source