EMAGE mouse gene expression spatial database

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EMAGE:30483

St8sia3 ( MGI:106019)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005497_01	euxassay_005497_02	euxassay_005497_03	euxassay_005497_04	euxassay_005497_05

euxassay_005497_06	euxassay_005497_07	euxassay_005497_08	euxassay_005497_09	euxassay_005497_10

euxassay_005497_11	euxassay_005497_12	euxassay_005497_13	euxassay_005497_14	euxassay_005497_15

euxassay_005497_16	euxassay_005497_17	euxassay_005497_18	euxassay_005497_19	euxassay_005497_20

euxassay_005497_21	euxassay_005497_22	euxassay_005497_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9585
Entity Detected:	St8sia3, ( MGI:106019)
Sequence:	sense strand is shown >T9585 ACACAACACACTCACACGCCAGCAGGCTGCTGGCCGCTCAATGGACCGATTTCCCCGGTTTCCCTGAACC CAGCCTAGCCCGGGATGAGAAATTGCAAAATGGCCCGAGTCGCCAGTGTGCTAGGGCTGGTCATGCTCAG CGTGGCCCTGCTGATTTTATCGCTTATCAGCTACGTGTCTCTGAAAAAGGAGAACATCTTCACCACTCCC AAGTACGCCAGCCCGGGGGCGCCCCGAATGTACATGTTCCACGCGGGATTCCGGTCACAGTTTGCACTGA AGTTTCTAGACCAGTCATTTGTGCCCATTACGAATTCTCTCACCCATGAACTCCAAGAGAAACCTTCTAA ATGGACATTTAATCGGACAGCGTTTTTACATCAAAGGCAAGAAATTCTTCAGCATGTCGATGTAATAAAA AATTTTTCTTTGACCAAGAGTAGTGTTCGGATTGGACAACTAATGCATTATGATTATTCCAGCCATAAAT ATGTCTTCTCGATTAGCAATAACTTCCGGTCCCTGCTCCCAGATGTGTCGCCCATTATGAATAAGCGTTA TAATGTTTGTGCTGTGGTTGGAAACAGTGGAATCTTGACAGGGAGTCAGTGTGGACAAGAAATAGATAAA TCAGATTTTGTTTTTCGATGCAATTTTGCCCCGACAGAGGCTTTCCACAAAGATGTTGGAAGGAAAACCA ACCTCACAACCTTCAATCCGAGCATCTTAGAGAAATATTACAACAATCTTTTAACCATTCAGGACCGTAA CAACTTCTTCCTCAGTTTAAAAAAGCTTGATGGGGCCATACTTTGGATCCCTGCATTTTTCTTCCACACT TCTGCAACTGTAACGAGAACGCTAGTGGATTTTTTTGTTGAGCACAGAGGTCAGTTAAAGGTCCAGTTGG CTTGGCCTGGAAATATCATGCAACATGTCAACAGGTACTGGAAAAACAAACACCTGTCACCCAAACGACT GAGCACAGGTATCCTAATGTATACTCTTGCATCTGCAATATGTGAAGAGATCCACTTGTACGGTTTCTGG CCCTTTGGATTTGACCCCAACACCAGGGAGGATCTGCCCTACCACTACTATGACAAAAAAGGAACCAAAT TTACCACCAAGTGGCAGGAGTCTCACCAGCTGCCTGCTGAGTTTCAGCTGCTCTATCGAATGCATGGGGA AGGGCTCACGAAGCTCACTCTGTCACACTGTGCCTAAGAACTCCAAATGGAAAGTGCCAAACGGCTGATT AAAAAGTGCCCCCACCCCCAAACCAAATTGAATAGTCTCCAGAACAGAACCCATAGACAATCTGGCAAAG CCTGTCTGCCACTTACAAGGAAAGACGCCTTCTCTTCCTCTTTTGCACTGCTCTTTGAATGGTCTTAACA AACTTAGGACAGGTGCATTGAAGCCGTGTGATTTAGACTTGATTGGGAAAAGGTTATATTGCATTTGGAA GTATGCTGCACAGAGAATAGCTTGAAATAGTTCTAAGTTTGTATTTTAATAATAAACCGACTCCCATGTG AATGAGGAATGTGACTGTCATCTCCTCCTCTCTACTTTGATATAGTCCTCACAACCAGGGAGCTCTGGCC AGCTCCAGCAGGATCTCTTTAGCCAAGGGGATCAGAATCTTCAAAATAAAAAGTAAAAAAACTGGTTTGC AGGTTGCATTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCACACACACACACACACACACA CACACACACACACACACACTTCCTGTTGCCCTAGTACCAATTAGGAATACTTCTGGGCTAGCTTTGCTTG TATGTGTTTGCATCTGTACCCATTGTGTTCAAGGCTCTGGATAACTTGGATGTTTCATAACAAACTGAGC AAATCTTAATGTCTTCCCCCGTGAAGAATTCTATAGGAAATGCCTCTTATTCTTACTGGTCCATATTGTT ATTCTACAGCATGGTGGTCTATTTGAGTAGAGTGGCATAAGCTACAGTGTCTCTTCTTTCTGTCAAAAAG AAAGCCCACAGTGTGGGTAGCATTGTGTCTGTTGGCATGAGCGAAGTTGTGGCAGATCCTGCAAGGGCTT TCTAGTGTAGCCTTCAACTATTTAATTTCAAGCGCTAGGCTGGGCACTCTCTCAGAAAAAAAAAAGAGTC ATTGGGTTAATTTAAACCCTAGGGACTATAAAGAAAGGGGAAGAAGAGTGTTTGCAGGTTGTATACTCTG CAAAGAATTGTGACCATTGGAGACATTGGAGAAGCACAAGAGTTGGTGTAGAAGACAAACTAGAGCTTTG GCCAGACTGGTCCCTGTGTGACGCATGTTAGCATTAGTCATGTCAGATCCTTTCTGTGACATCTCCACCA ACAAGGATCTAGACATGCTTGCTTTCTTTGAAACAGTTGAAGATATTATTCTCTGTTTCCAAGTCTGTGT GCCTATACATTGAGCATCTCAAAAAAGAAAAGCCATTATGCAGTGGGAATACTTGGTCCTAAGTAACCTT TGGGATTTGAGATCAGCTGGCCATTGTTCTCACTGCTTCAGCTGTATCCGTAGAAATCACATCGCTCCCA TCCCAGGCGTTTACACTGAGCAGCACTTTGTTCTTGCAATTCAACCCAAAGTCTGGCCAAGTTGATGTTA AATTTTCATAAGACTTTCCTCTTTACAAGGGCCCATGGAGCACATTTGTTATATGTTCCAGTGGGTTATC TTGTTCTTGTCCAGAAATCTCATCTAGACAGCTTACAGTGTTGTAAATATTTTTAAAAGCCCTTGCTATA GTTCAGGCATCAGTGTACTGGCCCTTTTTGGGAAAAAACAAAACCAACCATTCCTAAAAAGAAGTGGAGT AAGGACATGCACTGTTCTTGTGTAGCGCCTGGTCCTGGAGCTGCACTGGTCTTTTCGAGGAGATAAATCG ACCCCAGATAAATCTAGTGAAAGAAAGCCACTCAGATCCAAACACAATGCCAACTGTGGCACATCTTACC CTTTGTGAATGCCAGCCACTTAGATAGAGGAGAGGAACTAGGGGCTAAATATTTCAAAATAAGAAGCATT TACTATGCACAGGATAGTAAGAAAATTAAAATTATAAGGTATTTTTAAAAACACTTTAGAATTCAAGGAC AATTTCATGGGCATGAAAATTATACCATGGCTGTGCCCTGTCACAGTAAGCCACACCATGGCTGTGCCCT GTCACTTTAAAGAGTGCAGGACACCATGCTGCTACTCTGGATGGGTGTGGGTCATGGAGACTTGACAGAA TTGCAGCAGTTATCATCAGGACTGAAAAGCCTAGCTATTCCTAAGCCCTTAGCTCATTTGGTTGCAGCTA AGTTATTCCAATGCTAACATTATCCAGGCCAGATTCTGACTCGAAAGCTTTAATTTCAGTGTGCCCATCA GGTCTTACTAAATATAAACTTGGACTCTTTACACTAAGCAAGACAAAATACTCAAAGACTAGAAGATCCC CAAAGCTAGTTGAATGTTGTGGTGTTTTTTGTTATTTTTTTTAATGCACCTTTTAACCTCAGTTGTATCC TGGACCTGCTGTTCTGACCAACTGATTCTACAATTTTTTTTTTTCTGTTTCCCAAAACTATTTCAACATG TAAACCAATGCAACAGCTTTCATGGCAGGAAGATTTTCTTATTAGAAAAGCAGCCAGAGTTTGTTGGCTC TCCCACAGTCCCCAGGCTTGTAGGCTCAAATGCAGTGGACAGAGCAGAGGGACCATTGAGTTTCCTGTTT ATGCAGCTACACGCTCATACCCCACCCTTCTATGTTGGCTCCACTTGGTTGGATCTGGGTTAGTACATTC TTTTCTTAGAAACATACTCATTTTTTTCGGCTACCTAGAGCTGAGTACACCATCAGCTATGTGAATAAAT TTCACTATAATGCTCCCAAGGCTGTCTGAAAATAACTACTTACTTAGCAGAGAAGACAAGGAAATTTACA AACTCCTGGCAGCATGAGTCAAAAGGTGGTTGCCATGACTTCCTCCCACACCCGGAAATTTGGGGTCTGT GTGTGAGTCTATGGAGCTGGTGATCCAACCCAGGACCTTGCTCATGGTAGACAAATACTCTACCATTGAT CTCCATTCCCAGACTGTGTTCTCATGCTAAGCAACTTTAACCGTTATTTTTAAAAATGCAGTCTATCTAG TAGTACTGTGTGTCTGCCAATGCTGAATATGACCACTGAACCATCTGTCTCTGACCTGAATATCAGACAT GGGCTATAGCAAGGATATACAAAGGAAGACCCCGTTCATTCACCTAACCTTCTGGTACCAAGGCTGTCAG TGCCAATGGATATGAGTGTCCAGACAAAACTAATATATCAGAGAATTCAATGAATATGAGTCCATGCTAT CTCACCTCAAGCAAGGCCCCAAACACTCAGACAATAATTCATGAGCCCCAGAGAAGCAGGAACAATAGAA AGGGAGAAGTCATTGAGTTTTCTAACCCATGTTGGCTTTACAAATATTAAGGTTGAGATACAGATAGACT AATAGATTTTCCCCAAAATTTTACATAGATAATTATTGACGTTATGATTTCACATATTAATCTTATCAGA ATCAAAGATCAGGTCAAAGGAAAGCAACTTAACTCTGGAATATTTGGAGCGTAAACATAATGGAGAGGGG CCGTAAGGGAAGAAGGGGAAAAGACTACTAAATGCACTTGCCTGGGTACACAGCAGTAGGGGAGATACCT GCCCAGTACACAGCAGCCTGCTGAAAACCACCCACTCTCCCCTAAAATGAGAGAGAAACCTGTTAATCCT TGACTGATAAGTCCCAGTTCTGTTATTGATGGTACACAGCTGACAGATGAAATTGTACCTGATAGTATAT TCATCTCAATAAAATCATGTGTGCATGATTTAGAATTTGAAGGCTAAAAGTGGCTGTGTACTTCTCCTTG TATATAAAACTTTCAGCAAGGGCCCCTTGAGTTAAGAAACATTTTCCATCATGTAAGTAATCGAGACAAC AGCCTTTGAACTATTGTATTCGTTCATTTCACTAAGATTGTACAAAGCATAGTGGAAAACTTTGGTGGTT CTTCATTTTTTCAAAAGACATTCTGAGATCTTGTCTGTGTCGTTCTGGGTCTTTAAGCCCCTCTTCTCAT AACTGTTTGAATGTATCAGACAAATATTTGCTTAGCTTCTACTACTTCCCCACTGTGTATAACTGACCTT GTGACCAGAGTTGGGACTGTGTATGTGTGACTGAAGAGCTCTAATGGAACACCATGGGTTCCGGGAATCG AAGGCATTTCACCAGTCAGTCTAAGCCGCTTAATCCGTGGACCTTTTAACCGAGGCCACCAATGAATTGA TACCAAGGACCAAAATGACGAATCCTGCCTCCCTCACACTCTGACTGCTTTGCCACATAAATAGAACATT TCAAATGGGAAATGTTTTCGAATGTTATGGTTTGTTATGTGATATTTGTTGAGCATTTATTTTTATTGTA ATGATTTGTTAAAGACATGTGTGGTTGAATAGATGTTTTAATCCTTAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:30620472 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30620472 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005497 same experiment
	EMAGE:30481 same embryo
	EMAGE:30476 same embryo
	EMAGE:30353 same embryo
	EMAGE:31447 same embryo
	EMAGE:29534 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source