EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30534

Pbrm1 polybromo 1 ( MGI:1923998)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_010016_01	euxassay_010016_02	euxassay_010016_03	euxassay_010016_04	euxassay_010016_05

euxassay_010016_06	euxassay_010016_07	euxassay_010016_08	euxassay_010016_09	euxassay_010016_10

euxassay_010016_11	euxassay_010016_12	euxassay_010016_13	euxassay_010016_14	euxassay_010016_15

euxassay_010016_16	euxassay_010016_17	euxassay_010016_18	euxassay_010016_19	euxassay_010016_20

euxassay_010016_21	euxassay_010016_22	euxassay_010016_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31765
Entity Detected:	Pbrm1, polybromo 1 ( MGI:1923998)
Sequence:	sense strand is shown >T31765 GAGAGCCAGGTTCAGGCAGAAGACTTTGCGATCTATTTATGGTTAAGCCATCCAAGAAGGACTATCCTGA TTATTATAAAATCATCTTAGAGCCAATGGACCTGAAAATAATTGAGCATAACATCCGAAATGACAAATAT GCAGGTGAAGAAGGAATGATGGAAGACATGAAACTCATGTTCCGCAATGCCAGGCACTACAATGAGGAGG GCTCCCAGGTATACAATGATGCCCATATCCTGGAGAAGTTACTCAAAGATAAAAGGAAAGAGCTGGGCCC TCTGCCTGATGATGATGACATGGCTTCTCCCAAACTTAAATTGAGTAGGAAGAGTGGTGTTTCTCCTAAG AAATCAAAGTACATGACTCCAATGCAGCAGAAACTGAATGAAGTGTATGAAGCTGTAAAGAACTATACTG ATAAGAGGGGTCGCCGCCTTAGTGCTATATTTCTAAGACTCCCCTCTAGATCAGAGCTGCCTGACTACTA CCTGACCATTAAAAAGCCCATGGACATGGAAAAAATTCGAAGTCACATGATGGCAAACAAGTACCAAGAC ATAGATTCTATGGTAGAGGACTTTGTCATGATGTTTAATAATGCCTGTACCTACAATGAACCAGAGTCTT TGATCTACAAAGATGCCCTTGTACTGCATAAAGTCCTCCTTGAGACTCGGAGAGACCTGGAGGGAGATGA GGATTCTCATGTCCCTAATGTGACGTTGCTGATTCAAGAGCTCATCCATAACCTTTTTGTGTCAGTCATG AGTCATCAGGATGACGAAGGGAGGTGTTACAGCGACTCCTTAGCAGAAATTCCTGCTGTGGATCCCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3710414), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 59410. Forward Primer - name:059410_F_IRAV95_g05_Pb1, sequence:GAGAGCCAGGTTCAGGCA; Reverse Primer - name:059410_R_SP6_IRAV95_g05_Pb1, sequence:GTTGGGATCCACAGCAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_010016 same experiment
	EMAGE:30845 same embryo
	EMAGE:30839 same embryo
	EMAGE:30540 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source