EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30567

Leng8 ( MGI:2142195)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005098_01	euxassay_005098_02	euxassay_005098_03	euxassay_005098_04	euxassay_005098_05

euxassay_005098_06	euxassay_005098_07	euxassay_005098_08	euxassay_005098_09	euxassay_005098_10

euxassay_005098_11	euxassay_005098_12	euxassay_005098_13	euxassay_005098_14	euxassay_005098_15

euxassay_005098_16	euxassay_005098_17	euxassay_005098_18	euxassay_005098_19	euxassay_005098_20

euxassay_005098_21	euxassay_005098_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6335
Entity Detected:	Leng8, ( MGI:2142195)
Sequence:	sense strand is shown >T6335 GCCACTCTGTGGGCAGCTTTCAAGCTCTGGCCTGTGTTGGCCTCACTCTAACAGCACCTTTGCTGTGAGC TGAGCACAGCAGACAGATCCCTCAGATATCAGAGGCAGGCTGTTCTGTCCCAGGGCCATAGGGTGCCTGG GTTCCAGATACTTGGTAAGGCTTAAAGTGGTCTTCAGGAGAGGGTCCTCAAAATTAGCGGTACATGTACA CTATTAGGGCATCTGAGTAACAACCAGAAGGCACTGCTGAGTGTAACACTCTGTGCAAACATCCGGGGGG GAAGGGGGGGTAGGGGGGCGCCATTGTCCCTACCCTCTGAGCCCATGTACCACTTACTCTGTCTGGCTCC AGCTCCTCAGTCAGCAGAGGAGCCAAGCTGGGCCCTTTCCCTCCTGCCCCCTCCTTCCTGAACAGGTAAG TGTGGGCGAGGGGGGGCACAGCTGGGCACTGGGGCTACCAACCCCATCCCTTGCCCCCCCAAGCCCCTGA AAATGGGGAGGTCGGGAGGGGCTGCAGAAGCCAGCAGGTTCCTTGCTGAGGAAAATACAAGCCCAGATGG GGAGCATTGCTCCTTGTTCTTGGG
Notes:	The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005098 same experiment
	EMAGE:31352 same embryo
	EMAGE:31782 same embryo
	EMAGE:30808 same embryo
	EMAGE:30612 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source