EMAGE mouse gene expression spatial database

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EMAGE:30649

St6galnac2 ( MGI:107553)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003633_01	euxassay_003633_02	euxassay_003633_03	euxassay_003633_04	euxassay_003633_05

euxassay_003633_06	euxassay_003633_07	euxassay_003633_08	euxassay_003633_09	euxassay_003633_10

euxassay_003633_11	euxassay_003633_12	euxassay_003633_13	euxassay_003633_14	euxassay_003633_15

euxassay_003633_16	euxassay_003633_17	euxassay_003633_18	euxassay_003633_19	euxassay_003633_20

euxassay_003633_21	euxassay_003633_22	euxassay_003633_23	euxassay_003633_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6852
Entity Detected:	St6galnac2, ( MGI:107553)
Sequence:	sense strand is shown >T6852 GCCGGGGGCTCGGTGAGCAGGGCGGCAGACGGGGCGCACCCCGGCCAGGCTCACATGGACCTCCCGCGCC GCTGGCTCTTTCGGATGTTGTTGCTCGTTGCCACCAGCTCTGGGATCCTCCTCATGCTGTACTCCTCGGC GGGGCAGCAGTCCCCTGAGACCCAGGTTCCCGCCAGGAACATGGCGTACCCACGAGCATTCTTTGACCCC AAACCACCGAATTCGGAAAACAGAAAGAGCCGACTTTGCCAACACTCGCTGTCCCTGGCCATTCAGAAGG ACCGTCGCTTCCGCAGCCTGTTTGACCTCTCCACCCCGGTGCTGCTGTGGGAGGGCCTCTTCACTCAGGA ACTCTGGAATAATCTGAGCCAGCACAAAGTCCCCTACGGATGGCAGGGGCTCTCCCACGAAGTCATTGCC TCTACCCTGAGACTTCTGAAAAGCCCGGAGAGCGGGGAGCTGTTTGGTGCCCCCAGGAAACTACCTCTAA GCTGTATTCGCTGTGCCGTGGTGGGTAACGGAGGTATTCTGAATGGGTCCCGGCAGGGCCAGAAAATCGA TGCTCATGACTATGTCTTCAGACTCAATGGCGCCATAACCGAAGGCTTTGAGCGCGACGTGGGCACTAAG ACCTCCTTCTACGGTTTCACCGTGAACACCATGAAGAACTCGCTCATCTCCTACGCCAAACTGGGCTTCA CCTCTGTGCCACAAGGGCAGAACCTGCGCTACATCTTCATCCCCTCCAGCATCCGTGACTACCTGATGCT GAGATCCGCCATCCTGGGTGTGCCTGTTCCAGAAGGTCCTGATAAAGGGGACAGGCCTCACACCTATTTT GGACCGGAAACCTCTGCCAGTAAATTCAAGCTCCTGCACCCAGACTTCATCAGCTACCTGACAGAGAGGT TTCTGAAATCCAAGCTGATTAACACACGATTTGGAGACATGTATATGCCTAGCACGGGAGCGCTCATGCT GCTGACAGCCTTGCACACCTGTGACCAGGTCAGTGCCTACGGATTCATCACAAACAACTACCAGAAATAC TCAGACCACTATTTCGAACGAGAAAAGAAACCACTGATATTCTACGCAAACCACGACCTGTCCCTGGAAG CTTCGCTATGGCGAGACCTGCACAACGCCGGCATCCTTTGGCTGTACCAACGCTGACCCCAAAGTACCGG ATTTCTGTGCCTCAAAAGCACTTTTTTTGACCTTTCAACTTCTGGAAGAGGCAACACTCCCCTTGGCCCT CTACTTCCCAACAGAGGGTATTGAGACAAGACCCGTCACATAATACCAGAGCCTGTTCAATGCACGACAC TGTCACGGGTCAAGACAACTCTTCTGAGGCTTAGCCGAGGGAGGGAAGACTGCTTTGTAGGATTCGTTTT AAAACTGAACATTAAATGAGACAATGATGCTCACCAGCAAACAGGCGGGCAGGCACATCTCAGAGCTAAG TAGTCCAAAGCCAGCACCTGGCCCTGGGCAACTAGAAACATTGATGGAACCACTCAGGGCTATCCAGGAA CCTCGATCGAGCAGAGCTGAAACCAATGGCATGTTGAGTCCCAAATGCCAAAGCCATTCAACCTAGAACA CCTTAGGCATTATAGGAACAGGACACTTCTGCCTCCTGATAGAGGATGAGAAACAGAGGCAGAATGTAGT TTGAAGCTAGATAATAGGGTGTGATGGATGACCATTTTGTCTTTTTTGTGTTGGTAGAGTAGAAGACACA AACTCCAAGGTGGTGTGTCTGGAGGGAAGGGGTGTGGTGGCACTCAGGACAGAGTTTGGTGGCACCTGCA CCGAGGTTCAGGCTAGGAGTACCTAGCACCAAAGAGACAAACTTTGTGTAAGTCGCCACCACTGAGTCTC ACTGGAGAGGCACATCAGTCCTTGTCACCAACAGGAAACTGAGTGATGGACCCTCACTTCACAGCTTCAA TAAACAGCACCTGATGCAGTCCAATGAGCTTTTCCACATTTCCAGTCTCATCCAACCAAATTATCATGCA GCCCCTAAGCTGAAGGGCTGGGACGGGGACTGGGTATCTCTAGGGACTAAGGACCAGATAACCTCAAAAC AGCCCACCCACACTGGCCAACTCACCAAAGCTGAGGACCGGCCGACCATTGGTTCTGTGGCTTGGGAAAA CTGCTGGTGACGGCTGGGAGTGGTGGTCAGCGCACTTATTCCCAGTACTCAGGAAGGGCGAACAGACCAG CCTAGGGCATCTAACAGACTAAAACCAAGCCCAAGTGGCAATGGTGGCTTCCATCTTGACCACAACAGAA GGGGCTAAGTCGGATAATGGCTTGAGACCCAGTCTGGGGTACAGAGCAAGTCAGCCTGGGAGTTGGTTAG CCGTAAAAACCACAGCCTCTTGCAGCAGGAGTCGAATGGCTGTCCTACCTTTTGACGGACTCCATCAGAT AGCCCTGTCAGTTCTGCAGACTACCCGTAGACCCTCAGTACACTCCCCATCAAATGTGCAGACACACGAG TGTCCTTTACCAATGCTCCATTTAAGAGCTATTGTTTATACATGAGGTTTTGCCTGTTGTGTTGGCCTGT GCATTACATGCCTGGTACCCAGGATGTGTCCACCAAAGGATCCCTTAAGGCTAGGGTTACCGATGGCTCT GAGCACCATGTAGGTGCTGGCAATTGAACCCAGGTGTTCTGCAAGAGTAGCCTGTGCCCTCCAGCACATT ACCACCGGATGGTTGAGGTGTTATGCAATGTTAACCTATGCTTTAAGTGACAAGTAAATTTTAGCAAGTC AATAGAAAAGGTCTGATTCTCTACAGTTCAGTGCAAGTGGTCCTTAAGTATTTCTAAGGCTCACTCATAT ACCCACAATGCTACCAAGTTAAATTTTATAAAGTTTATTTGTGCTTCAAAATACAAAGGCCAACCCTGAC TTCCAGGAAATCCACTGTGAGAAATGCTTTTCTCGGCTTAGCAGGGGCCTCCCCAGGGCCTCCGACTGAG CGCCCTCACCAGCTAGCTGTCAAAGGCTTCCGAGGGTGACCACAGTCACGAGGCCTTACTGGCTCCTCTT GCAGAACAAACTCCAAGGTCGTCTGATGACTCTTTGGGAAATTAAAGGCAGGAGCAGCAGCAGGCTATGT GGTTCTCTCTGGCACTACAGGGCAATAACCTAGAAACAAGGAAAGAACCTGAAGGAGACAGGCAGCTAAG TGATCAAAACTACAAACTTTAGGAACAAGCATTATCAGATAGTCCTGGGATTTCAGCCGCCATCACAAGG CACTGTGTGCGCTACACACAGGCAGGCACCAGCCAAGTTCAGAAGCAGGCTGCTAACTTTTTTTTTAAAA GAAAGTTTTAAGAAGTTCCTGGTAGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGCTCTTC CGAAGGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGTAATGAGATCTGGCGCC CTCTTCTGGAGTGTCTGAAGACAGCTACAGTGTACTTATAA
Notes:	The probe template was PCR amplified from IMAGE:3964698 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964698 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003633 same experiment
	EMAGE:32000 same embryo
	EMAGE:30657 same embryo
	EMAGE:32029 same embryo
	EMAGE:30656 same embryo
	EMAGE:30697 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source