EMAGE mouse gene expression spatial database

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EMAGE:30721

6330406L22Rik ( MGI:1917969)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004131_01	euxassay_004131_02	euxassay_004131_03	euxassay_004131_04	euxassay_004131_05

euxassay_004131_06	euxassay_004131_07	euxassay_004131_08	euxassay_004131_09	euxassay_004131_10

euxassay_004131_11	euxassay_004131_12	euxassay_004131_13	euxassay_004131_14	euxassay_004131_15

euxassay_004131_16	euxassay_004131_17	euxassay_004131_18	euxassay_004131_19	euxassay_004131_20

euxassay_004131_21	euxassay_004131_22	euxassay_004131_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7826
Entity Detected:	6330406L22Rik, ( MGI:1917969)
Sequence:	sense strand is shown >T7826 CCCAGGTCCCTTGACCACGTACTAGCGTTCTGTCCCTCCATACATTACTGAGCAATGTGTGTCTGTGGGA CTCTGCATCCAGCGCTGGACCATGACCCCCTGTGCTGCCAGACTCGCGCCCGAGACCTTCTCGAGGCCCG CCGGCCACTGGCCCATGAGTGCCTGGGTGAAGCTCTGCGAGTCATGCGCCAGGTCATCTCCAGATACCCA CTCCTCAACACCGTGGAGACACTCACAGCCGCTGGTACACTCATTGCCAAGGTCAAAGCCTTTCATTATG AATGCAACAATGAATCAGATAAGAGGGAATTTGAGAAGGCTCTGGAAACCATTGCGGTGTCCTTCAGCTG CACTGTGTCCGAGTTCCTTTTGGGTGAAGTGGACAGCAGTACTCTCCTGGCGGTGCCTCCGGGGGACCCC AGCCAGTCCATGGAGAACCTTTATGGGGCTGGCACGGAGGGGCCCCCACATAACGTGGAGGAATGTGAAG AAGGTTGCCTGCCCCCCGAAGAGGTGGACATGCTTCTGCAGCGATGCGAAGGGGGTGTGGACGCTGCACT GCAGTATGCAAAAGACATGGCCAGGTACATGAAGGACCTCATCAGCTACCTGGAGAAGAGGACCACCCTG GAGATGGAATTCGCCAAAGGTCTCCAGAAGGTTGTCCACAACTGCAGACAGAGCGTCACACACGAGCCCC ACATGCCTCTCTTGTCCATCTACTCACTGGCCCTGGAACAAGATTTGGAGTTTGGCCACGGCATGGTGCA GGCAGCGGGCACACTGCAGACCCAGACCTTCATGCAGCCCCTGACGCTGCGGCGGTTGGAGCATGAGAGG CGCAGGAAGGAGATCAAAGAATCTTGGCATCGAGCACAGAGGAAGCTGCAAGAGGCAGAGGCCAACCTGC GCAAGGCCAAACAGGGCTACAAACAACGCTGTGAAGACCACGACAAGGCCCGGCTCCAGGTGGCCAAGGC TGAGGAGGAACAACAGGGCACAGGCCCAGGCGCAGGGACTGCGGCCTCTAAGGCCCTGGACAAGAGGCGC AGACTGGAGGAAGAGGCCAAAAACAAGGCCGAGGAGGCCATGGCCACTTACCGCACATGCGTGGCAGACG CAAAGACACAGAAGCAGGAGCTGGAGGACACAAAGGTGACTGCGCTGCGGCAGATTCAGGAGGTCATCAG GCAGAGTGATCAGACCATTAAGTCGGCCACCATCTCCTACTACCAGCTGATGCACATGCAGACAGCACCG CTGCCCGTGAACTTCCAGATGCTGTGCGAGAGCAGCAAACTATATGACCCGGGCCAACAGTACGCATCTC ACGTGCGTCAGCTGCAGAGAGGCGAGGAGCCGGACGTGCGCTACGACTTTGAGCCTTATGTCTCTAACAA CTCCTGGTCCCCAATCATGCGTACACGGAAGGGCAGCTTCAACCCTGGTGATGCTTCAGGACCCGAAGCT GCTGGTAGTCCCCCCGAGGAAGGTGGCACCTCTGAGGCAGCACCCAACAAGGACCACAGGGGGGGACGAG GTCACCAGGTTCATAAGTCCTGGCCCATCTCCATCTCAGACACCGAAGTTGGTCTGGACACCAGCTCAGG GGACTTGAAGAAGTTCGATCGAACATCTTCCAGTGGGACGATGTCATCCAGTGAGGAGCTGGGGGATCAG GAAGCTGGCTTGGTAGCTTCCGCCTTTGATTCAGCTGACCTCAATGGCATGGACCCCGAGTTACCTGTGG CCATGCCCAGTGGACCCTTCCGCCATGTGGGATTGTCCAAGGCAGCCCGCACACACCGACTTCGCAAACT GCGCACGCCAGCCAAGTGCAGAGAGTGTAACAGCTATGTGTACTTCCAAGGAGCTGAATGTGAAGAGTGC TGTCTGGCTTGTCACAAAAAGTGTTTGGAGACCCTGGCCATCCAGTGTGGCCACAAGAAGCTTCAGGGCC GCCTGCAGCTCTTTGGACAAGACTTCAGCCAGGCAGCCCTCAGCACCCCTGATGGGGTGCCCTTCATTGT CAAAAAATGTGTCTGTGAGATTGAGCGGCGGGCACTGCACACCAAGGGGATCTACCGGGTCAATGGCGTG AAAACGCGTGTGGAGAAGCTGTGCCAGGCTTTTGAGAATGGCAAAGAGTTGGTGGAGTTGTCACAGGCCT CACCCCACGACATCAGCAACGTCCTAAAGCTATACCTGCGGCAGCTACCGGAGCCCCTTATCTCTTTTCG CTTCTACCATGAGCTGGTGGGACTAGCTAAGGACAGCCTAAAGGCCGAAGCCGAAGCTAAGGCAGCGAGC CGGGGTCGGCAGGGTGGGTCCGAGAGTGAGGCTGCAACCTTGGCTATGGTGGGCCGCCTGCGTGAGCTCA TGCAGGATCTGCCAGCCGAAAACCGGGCCACACTCTTATACCTGCTGAAGCACTTGCGAAGGATCGTGGA AATGGAGCAGGATAACAAGATGACCCCTGGGAACCTGGGCATCGTTTTCGGGCCCACACTGTTGCGGCCT CGACCCACTGACGCCACCGTGTCCCTCTCTTCTCTGGTGGATTATCCCCACCAGGCCCGAGTTATTGAGA CTCTGATTGTCCACTACGGCCTGGTCTTTGAGGAGGAGCCAGAGGAGGCGCCTGGCAGCCAGGAGGGGGC ATCCACCCAGTGTGGCCAGCTGGAGTCTGCTGAGGGCATTGTCTTCCCCCTGCAGGAGGAGGCAGAGGAC GGAAGCCGAGAATCTCACGCGGCATCTAATGACTCGGACTCTGAGCTGGAAGACGCTTCTGACCCCCTTT CATCCTCGGACGCCAGTGCTCTACACCGCCTCAGTTTCCTGGAGCAGACCGAGGCAGGCCTGGAGGAAGG TCCCCAGAGCCATAGTGGCAGTGAGGAACAGCTGGAGGGTGAGGATGGAGCCCCGGGTCCGTGGCTGTGT CACTTCAACACCAACCAGTCCAACAACACATCACGGGCCCCTCTGCCCACCATGCGGCTCCGAGGAGGGC AGATCACAGGTGGCACCAGTCAGGAGCGGCAGCCACAGTTCGTCTGAGCTGGGTGGTGATGGCAGGGACC ATCAGCTCAATGTCAGCCTTTGACTCGGGGGGAGGGGGGTGTTCCTCACAATGCCGAGGAACTTCAGGGA GATCAGGGCTCCCTGGTACCATGGACACAGATGGATAACAGTCCCAGATGGAGTTGCCTGGCCTTTGTGT GCCTGTGGGGATGCAGCTGCAGATTCCCATGATGGCCTCATCTCTCCCATGGTCCCCATCAGTCTGGATG TGTCTGAGCGGGTACAGCCCTGACCTCTGTGTGTGGAGCTGGAGGTGGCTCTGGCTTCAGGAATCTTGCA GGAGGCTGGACATGGACAGACGGGTGGGGGCACACAGTGGGTCTCTCCCAAGGAGCAGTAAAGGAGCTTT CCACATGAACTCACAATTTTCTTGAAATAAACTTCTTAACCAACTGCAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6333971 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6333971 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004131 same experiment
	EMAGE:30515 same embryo
	EMAGE:30494 same embryo
	EMAGE:31629 same embryo
	EMAGE:32116 same embryo
	EMAGE:30511 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source