EMAGE mouse gene expression spatial database

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EMAGE:30786

Smurf2 SMAD specific E3 ubiquitin protein ligase 2 ( MGI:1913563)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_012164_21

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Expression Pattern Description

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37060
Entity Detected:	Smurf2, SMAD specific E3 ubiquitin protein ligase 2 ( MGI:1913563)
Sequence:	sense strand is shown >T37060 CTGACAGTGCCAAGATACAAGCGAGATTTGGTTCAAAAACTAAAAATCTTGCGGCAAGAACTTTCCCAAC AGCAGCCTCAAGCTGGCCACTGCCGTATTGAGGTGTCTAGGGAAGAGATTTTTGAGGAATCATATAGACA GGTCATGAAAATGAGGCCAAAAGATCTATGGAAGCGATTAATGATAAAATTTCGTGGAGAAGAAGGTCTT GACTATGGAGGAGTTGCCAGGGAATGGTTGTATCTCCTGTCACATGAAATGTTGAATCCTTACTATGGTC TCTTCCAGTATTCTAGAGATGATATCTACACATTGCAGATCAATCCTGATTCTGCAGTTAATCCGGAACA TTTATCCTATTTCCACTTTGTTGGACGGATAATGGGAATGGCTGTATTCCATGGACATTATATTGACGGT GGCTTCACATTACCTTTTTATAAACAGTTGCTTGGGAAGTCAATTACTTTGGATGACATGGAGCTAGTTG ACCCAGACCTTCATAATAGTTTAGTGTGGATACTTGAAAACGATATCACAGGTGTTCTGGACCATACCTT CTGTGTTGAGCATAATGCATATGGTGAAATAATCCAGCATGAACTCAAACCAAATGGCAAAAGTATCCCT GTTACTGAAGAAAATAAAAAAGAATATGTCAGGCTTTATGTCAACTGGAGATTTCTCCGAGGCATCGAAG CTCAGTTCCTGGCGCTGCAGAAGGGCTTTAATGAAGTCATTCCCCAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 83331. Forward Primer - name:083331_F_cDNA_Smurf2, sequence:CTGACAGTGCCAAGATACAAGC; Reverse Primer - name:083331_N_SP6_cDNA_Smurf2, sequence:CTGGGGAATGACTTCATTAAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012164 same experiment
	EMAGE:32183 same embryo
	EMAGE:30761 same embryo
	EMAGE:30634 same embryo
	EMAGE:31756 same embryo
	EMAGE:32224 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source