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EMAGE:30819

Psmd13 ( MGI:1345192)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
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euxassay_009532_01 euxassay_009532_02 euxassay_009532_03 euxassay_009532_05 euxassay_009532_06
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euxassay_009532_07 euxassay_009532_08 euxassay_009532_09 euxassay_009532_10 euxassay_009532_11
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euxassay_009532_12 euxassay_009532_13 euxassay_009532_14 euxassay_009532_15 euxassay_009532_17
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euxassay_009532_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
medulla oblongata alar plate ventricular layer
strong strong
regionalstrong expression: see section 05 06 07 08 19 20 21 22
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 12
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16 17 18
trunk mesenchyme
moderate moderate
regionalmoderate expression: see section 12 13
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 11 12 13
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36909
Entity Detected:Psmd13, ( MGI:1345192)
Sequence:sense strand is shown

>T36909
TAAACCCTCTGTCCCTGGTAGAAATAATTCTCCATGTGGTTAGACAGATGACCGATCCTAATGTGGCTCT
TACTTTTCTGGAAAAGACTCGTGAGAAGGTAAAAAGTAGTGATGAAGCCGTGATCTTGTGTAAGACGGCA
ATCGGAGCTCTAAAATTAAACATCGGCGATCTGCAGGCTACAAAGGAAACCATTGAAGATGTTGAAGAGA
TGCTCAACAATCTCCCTGGTGTGACGTCCGTTCACAGTCGTTTCTATGATCTCTCCAGTAAATACTATCA
AACAATCGGAAACCACGCGTCCTACTACAAAGATGCTCTACGGTTTCTGGGCTGTGTGGATATCAAGGAC
CTGCCAGTGTCAGAACAGCAAGAGAGAGCCTTCACACTGGGGCTTGCTGGACTTCTCGGAGAGGGAGTTT
TTAACTTTGGAGAACTTCTCATGCACCCTGTGCTGGAGTCACTGAGGGACACTGACCGGCAGTGGCTGAT
TGACACCCTGTACGCTTTCAATAGCGGTGCTGTGGATAGGTTCCAGACACTGAAGTGTGCCTGGGGCCAG
CAGCCTGACTTAGCAGCCAACGAAGCCCAGCTTCTGAGGAAGATCCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95374. Forward Primer - name:095374_F_cDNA_Psmd13, sequence:TAAACCCTCTGTCCCTGGTAGA; Reverse Primer - name:095374_N_SP6_cDNA_Psmd13, sequence:CTGGATCTTCCTCAGAAGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_009532 same experiment
 EMAGE:30806 same embryo
 EMAGE:30816 same embryo
 EMAGE:29216 same embryo
 EMAGE:29228 same embryo
 EMAGE:30851 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS