EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:30831

Stk40 ( MGI:1921428)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004092_01	euxassay_004092_02	euxassay_004092_03	euxassay_004092_04	euxassay_004092_05

euxassay_004092_06	euxassay_004092_07	euxassay_004092_08	euxassay_004092_09	euxassay_004092_10

euxassay_004092_11	euxassay_004092_12	euxassay_004092_13	euxassay_004092_14	euxassay_004092_15

euxassay_004092_16	euxassay_004092_17	euxassay_004092_18	euxassay_004092_19	euxassay_004092_20

euxassay_004092_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

esophagus

moderate

regional

moderate expression: see section 08 09 10

lower jaw molar

strong

regional

strong expression: see section 06 18

submandibular gland primordium

moderate

regional

moderate expression: see section 05 06 13 14 15 16

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 17 18 19 20

oral epithelium

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

upper jaw molar

strong

regional

strong expression: see section 06 18

thymus primordium

weak

regional

weak expression: see section 07 08 10 11

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 09 21

epidermis

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

lower jaw incisor

strong

regional

strong expression: see section 09 10 11 14

upper jaw incisor

strong

regional

strong expression: see section 11 12 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5574
Entity Detected:	Stk40, ( MGI:1921428)
Sequence:	sense strand is shown >T5574 GGCGGCGGCAGCTCTGGCGGCTGCGCTGGGAGGCGGCGGTGAGCGGCTCCCACGCCCCCGGCCCGGCCCC GGCCCCCGGGACGGAGCGCCGAGCAGCCCCGACTCTGGGCTGCGGACTATGGGCAAGGAGCGCTGATTCC CCGGGAAAGAACTGAGAATGAAGCGGAGAGCATCAGACAGAGGAGCTGGGGAGACGTCCGCCAACGCCAA GGCTCTAGGAACTGGCATTGCTGGAAATAACGCAAAGAGAGCAGGACCGTTCGTCCTGGGTCCCCGCCTG GGCAACTCACCGGTACCGAGCATTGTGCAGTGTTTGGCAAGAAAAGATGGCACAGATGACTTCTATCAGT TGAAGATCCTCACCCTTGAGGAGAGGGGTGAGCAAGGAATAGAGAGCCAAGAGGAGAGGCAGGGCAAGAT GCTGCTGCATACCGAGTACTCTCTGCTCTCGCTGCTGCACACACAGGATGGCGTGGTCCACCACCATGGG CTCTTCCAGGACCGCACCTGTGAAGCGGTCGAGGACACAGAGTCTGGTCGGATGGTAAAGAAGATGAAGA AACGCATTTGCCTCGTCCTTGACTGCCTCTGTGCACACGACTTCAGTGACAAGACGGCTGACCTCATCAA CCTGCAGCACTATGTCATCAAGGAGAAGAGGCTCAGTGAGCGCGAGACTGTGGTCATTTTCTACGACGTG GTCCGGGTGGTGGAAGCCCTGCACCAGAAAAACATCGTGCACCGAGACCTGAAGCTTGGGAACATGGTGC TCAACAAAAGGACCCATCGGATAACTATCACCAACTTCTGCCTTGGGAAGCACCTTGTGAGTGAAGGGGA CCTGCTGAAGGACCAGAGGGGCAGCCCCGCCTACATCAGTCCTGATGTGCTCAGCGGCCGGCCGTACCGG GGCAAGCCGAGTGACATGTGGGCCTTGGGCGTGGTGCTCTTCACTATGCTCTATGGCCAGTTCCCCTTCT ACGACAGCATCCCACAGGAGCTCTTCCGCAAGATCAAGGCTGCTGAGTACACCATCCCTGAGGATGGGAG AGTCTCTGAGAACACAGTGTGTCTTATCCGGAAACTGCTGGTCCTTGACCCCCAGCAACGCCTGGCTGCT GCCGATGTCCTGGAGGCTCTCAGTGCCATCATTGCATCTTGGCAGTCCCTGTCCTCTCTGAGTGGCCCTT TGCAAGTGGTTCCGGACATTGATGACCAAATGAGCAGCTCTGACAGCTCCCAGGAGGCAAAGGTGACAGA GGAGTGCTCCCAATACGAGTTTGAGAACTACATGCGACAGCAACTGCTGCTGGCTGAAGAGAAGAGTTCC ATCCATGAGGCCCGGGCCTGGGTGCCCAAGCGGCAATTCGGTAGCATGCCACCGGTCCGACGGCTAGGCC ACGACGCACAGCCAATGACCTCCTTGGACACAGCCATCCTGGCACAACGCTATCTGCGGAAATAGTGCCC TCAGCTAGGGCACTAGCCCAGCACACCTCTTCTCGGCCCACCTGAGGCCCAGCCATCGGGCCTGCCTGGG CCCGGGCCCAGCAGTGCTGGACTGATTCTCCCAGGCTGCAGTAGGGATAGGGCAGGGATAGGGACAGCCC AGGTCACAGCCAGGGTCAACAGAGGTACCACAGAGCTACCTTTTGGGATGATTGCTTGATTGTTTTTTTA TCTGAGAATTCTAGATAGATAGCTAATCTGCTTTTAATCATGACATTTTAATCTACCTCTGTCTCTTTAA CCACGCTGTCTCTGGACTGAGTGTCAGGAGGGAAGAGGGGAGTCTACGCTCACAACCCAGGCACTGGACA GCTGCAGCCTGGCTAGGCCACTGACCCCACAGTCCATATAAGCTGACCATGCAGCTCGCTGCTGGCCCCC AGTAAGCACCCCTTCCCACCGTCCACTCCCCGTCCCCGGGGGCCTGCCCTCAGTCTAAGCTTTGGAACAC TCTCACCTCACCTGCCGCCCAAGTCAAATCTATTTATTTTTTTACTGAACCCAACTTGTATCTCTAGGTT CAGCCCTTGGCCACTCTGGCCCTGAGCCTGGCTGAATAGCAGGGCCCGTGTGTGTAGGCTCCCTTCTCAC CCCAGGCACCTAGGAGCCCTCTTGCCAGTTCCTCTCTCGCTCTGTTTCCAGACCTGTCTCTCTCTCTGTC CTGGCAGCTTCTCTGAGGTGCTGCTCGTTTGCATTAGTCCCGCCTCCTCCATCTTTCATGTGGTACCCAG GTAAATGTGGACATAGTGTGAAGGGACCTGTGGCCACCAGAGGATGCTCTGGGCCTACTGAGGACAGGCC ACTTGTGCTCAGAGAACCTCACCTCACCGCAGAAGTGGTGCCTCTTCCAGAAGACTCTGGAAGATTACCT ACTTGGGGGTAGGCCTTGGAGGGAAATCTGTAGGGGTGGGGACCTTCCTACCTTCCTGGCTTGAGAACAG CCCTGCTACCCTTTTTGAGCCGAAATTTTGAAGTGGATGCCCGTCTTGCCAGAAACGCTGTTCTCTCCAG AATGCCCCTCCCTGCACTGGACTTGGCACACCCAAAACCAAGCAAAGACCCAACTCCGGAGGCCTACCTC CTGTGTACCCTCAGAGAGGGCAATGTCACCCTCCTGGTAGGTAGTCCTAGACCCCTCCTGCCTCTTGCTC TCCAATGGGGAGACCATGTTTCCTAGAGTGTGAGCTGCCCCTCACCCCCTGAAAAGCTGACTCACTGTGA GTGACCTTGGGCAAGTCCCCAAACCTCCTTGTGCCTCAGTTTCCCCATCTGGAAAAAATGGGGCCACCTC TTGCCAGCAGTAGCAGGGCTGCCCATGCCCCTTCCTCCCCATGCCCCATATCCAGCCCTGGGCTCCTGTG CCTCTGTTTTGCTGGGTCTGTGGAGCCCACTTGAGCCCAGCAGTTAACTCCCCTGGCACTCAGCTCTGCC TCTAGTTCAGCCGCCCAGCCACTGAGAGTCAGGGCATGGTGCTGCGGGGGAGGGCTCTTTTCTATATTTA TTTGATAGCGATAAGTCTCCGAAGGGAGTGGAAATGCAGTCAGCCTGGGCCCTTAGCCCATGACTACCCT CCAGTGAGGCTCTGAGGCCTGGCTAGGGCCCTCCTGGGCAGAGCCCCTGCCTAGCCCTTCAGGCCTGATG CCTCAGGCCTGATTCCCTCCTTTTAAAGCAATACCTTCAGGTCTGCCATGCCATCAGCCAGCCTGGTTCC TTCTGAGGGCAACCTGGGTCTGGTGGTATTTCTGGTGGTGCAGATGAGGGGAGGGTGGGGCCCTCGCTGC TGTCAGGCACTGAGAAGTCCTGTTGTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6478265 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6478265 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004092 same experiment
	EMAGE:29648 same embryo
	EMAGE:30829 same embryo
	EMAGE:29657 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source