EMAGE mouse gene expression spatial database

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EMAGE:30835

Slc2a9 ( MGI:2152844)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006278_01	euxassay_006278_02	euxassay_006278_03	euxassay_006278_04	euxassay_006278_05

euxassay_006278_06	euxassay_006278_07	euxassay_006278_08	euxassay_006278_09	euxassay_006278_10

euxassay_006278_11	euxassay_006278_12	euxassay_006278_13	euxassay_006278_14	euxassay_006278_15

euxassay_006278_16	euxassay_006278_17	euxassay_006278_18	euxassay_006278_19	euxassay_006278_20

euxassay_006278_21	euxassay_006278_22	euxassay_006278_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 09 10 11 12

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6567
Entity Detected:	Slc2a9, ( MGI:2152844)
Sequence:	sense strand is shown >T6567 CTTGGGAACAGTGATCCCACTGAGCGATACCTGGCATAGTAGAGTGGACTGCCGGCAGTCGCAATGGAGA CCTGTGGACCCTGAAGTTGATGGCGCTGGAGAGAGAGGGCTGGCAATGCTGTAACTCTCCAGCAGAGAGA AGAATGTGTGGCTGGGAATGTGATCCTGCTGCCATGAAACAGAGGTGCTGCCCGGAATAACAGAAGCCCA AGACCATGAAGCTCAGTGAAAAGAACTCCGCAGAAACCAAGGAAAGCCAGCGGAAATGGTCCTTCTCGCT CGTCGTGGCAGCTCTCGTGGGAGCCTTCGGGTCCTCCTTCCTCTACGGGTACAACCTCTCCGTGGTGAAC GCGCCCACGCCGTATATCAAGGCCTTTTACAATGGAACCTGGTACAGGAGGCATGGACAGCCCATTGACC CGGATACCCTGACTCTGCTCTGGTCTGTGACTGTGTCCATATTTGCGATTGGTGGCCTGGTGGGAACGCT GATGGTGAAGATGATTGGAAAGTTTCTTGGGAGGAAGTCCACATTGCTGGTCAACAATGGATTTGCCATT TCCGCAGCGTTGCTGATGGCATGCTCACTCCGGGCAGGAACCTTTGAGATGCTCATTGTGGGACGGTTCA TCATGGGTGTGGATGGAGGCATTGCACTCAGCGCGCTCCCCATGTATCTCAACGAGATCTCACCCAAGGA GATCCGGGGCTCTCTGGGCCAGGTGACTGCCATCTTCATCTGCATTGGCGTGTTTTCTGGACAGTTGCTG GGCCTGCCGGAGCTGCTGGGAAGGATCTGGTTCTACACCAACAGCATCTTTGGGAAAGCTGGGATCCCTC AGGACAAGATCCCATACATCACCCTGAGCACGGGAGGAATTGAAACACTGGCTGCTATCTTCTCTGGCTT GGTCATTGAGCGCTTAGGAAGGAGACCTCTCCTCATTGGTGGCTTCGGGCTGATGGCCCTCTTCTTTGGG ACCCTCACCGCGACACTGACCCTGCAGGACCAAGCTCCCTGGGTCCCTTACCTCAGCATTGTGTGCATCT TGGCCATCATTGCCTCGTTCTGCAGCGGTCCAGGTGGGATCCCATTCATCCTGACCGGAGAGTTCTTCCA ACAGTCAGAGCGACCGGCAGCCTTCATGATCGCAGGCACAGTCAACTGGCTCTCAAACTTCGCCGTAGGG CTCCTTTTCCCTTTCATCCAGAAAAGCCTGGACTCCTACTGCTTCCTCGTCTTCGCCACAATATGTATCG CAGGTGCTACCTACTTCTATTTTGTCCTCCCGGAGACCAAGAACAGAACCCATGCAGAAATCAGCCAGGC ATTTGCCAAGAGGAACAAGGCTCAGCCCCCAGAGGTGAAGGCTGATTCAGCCATGACTGAGGAGAAGGCC AACAGCCAGACTGAGCCCGATTCATCCTCCACGCTGGACAGCTATGGCCAAAACAAAATTGTCTAAATGT GTGTTCTTGCTATAGTAACCTAAGACTTTCCAAAACACTTAGAAGGTTTCAGGAGACCAAAGCCATGTGA GACTCTTATACTGGGGAGTTAGGAAGAAACCTGTGGGAATCGAACCTCCCAGGTGCACGTCCAAACTGCC CTGGAAGCATCCCCTAGGTTTCCTACACTCACCATACTATATACACCGAGGGCTGCCCTCCTCATGACTG AACCCATAGTTTTGAAATAAAGTTGGGGGAAAGGGAACAGGTTGCCTTCTCATAGGAAAACCATCTGGTC TGATGGACACTTACTTGTTGTACTGGGAATGGCAGCCTGGGGCTTGTCCCAGCTGTGCTGTTGCTAAGCA ACCCTGACCTTCCCTCAGCCTCTAGCCTCCAGGCCCCCAGACCTTGGAAGACTTTCCGACTTTCTCTTTC ACGGGAAGATTCTTTGTCCCACTAACAAATGCCTGTTGTTAATCTTTGGCTCTGCCCTATGGACCTGAGG CTTAGGAACACTTCATACCAACCCACCACGCTGCTGATCTTACCAAGGCAGGCTCTGCCCCCCAGCACTC AAGGCCTCGAGACCTGAGGCTGTCTTTAGTTCAGACTGTTACAGAGAGCACACAGAGAATCAACCAATCA TATGAAAAAGAACTTTGTGGTACCTAGAGGGGCAGAGCCTGCAAGGTGGGCCCTGAACACTGAGAATGAC TCAGACTACATCTAGATTTGATGGGAAAGAACACTGGGATTGATTGATGATGTCTGTCCTGGATGTAGGA ACGACAGCCTCAGTTCCCGAGCCTTGTGTTTGCAATGTGAAGGTAACAAACTGTCTGGAAAGACACCCGA CATCACTCCTAGTGCTCAGGTAAAGGCTGGGGTGGCAGGGGTCACCTGTCTCAACCGTTGTTTAGGAAGT CAAAGAGGCGGTTCTAGAGAAGGGTGTTTGAGTGGGTGTCTGGTTGGGGCAATTTGCATCTTGCTGTCGT CATGTTTGGCTGAATCCCTTCTTCTGCCTCCAAAACAAAGTACGTGTGACAAATGTCATAAGCAAATGGC AGGCACATGAACTGGCATTGGCGTGTTTAAGTTTAGGATGCCTTAGAGGGTATGTGGTTCCAGGAAGGGA AGTCTCCACACCAGGCAACACTGCTGTTTTCCTGACCGTTCTGGATGAACATGGCCTTAACCAAAACCTG TGTTCTGACTTTTCCCTCCCCGTTGAACACTCTTCATCTTGGGCCTCTCCTTGGTGCATTCCCGGCTCAG GGAACCATACCATCCAGCCCTGGTTTGAACACTGTGCGTTAGTATACAGAATGCAACATATAGATTTGAC AAGGGCTTGTCACAAAGGGAACCAAGTAATTGTAAAACAGAAATCATAACATTTGAAAGCTGGCATTCTC TTGGAGATTTAAGGATTGTGTCAATAAATATTTATTAGCCTATCATTATTCTGCCTTGGCTGGGTACTGC AGACAGAGCCATGGGCAAGGAAACACACTGCCCTGGCCTTCGCACTCTCATGTCTGACCGAGAAACACCG TCAAAGCACTAATGGTGGGATGCAGTGCCTTCACTGGGGGTGGGGGGGTGGGCATTGGTAGCGGGAATAG ATGGGATCTTAGCTGAGAAAGAGGAAAGCCTTCTCAGACAAGGGATGAACAGAGCCAGGGCCAGTGACCA TGAGGCCGGAGGCCAGCAATTATTATTGTTATGTTCTTGTATTTGAAATTGTAGTGAAGCCACACTAAGG GCATTTTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3593581 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3593581 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006278 same experiment
	EMAGE:30228 same embryo
	EMAGE:30254 same embryo
	EMAGE:31243 same embryo
	EMAGE:31259 same embryo
	EMAGE:29349 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source