EMAGE mouse gene expression spatial database

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EMAGE:30848

Abcb9 ( MGI:1861729)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_008420_01	euxassay_008420_02	euxassay_008420_03	euxassay_008420_04	euxassay_008420_05

euxassay_008420_06	euxassay_008420_07	euxassay_008420_08	euxassay_008420_09	euxassay_008420_10

euxassay_008420_11	euxassay_008420_12	euxassay_008420_13	euxassay_008420_14	euxassay_008420_15

euxassay_008420_16	euxassay_008420_17	euxassay_008420_18	euxassay_008420_19	euxassay_008420_20

euxassay_008420_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 10 14 15 16

facial vii ganglion

weak

regional

weak expression: see section 03 04 18 19

vagus x ganglion

weak

regional

weak expression: see section 07 15

thymus primordium

weak

regional

weak expression: see section 10 11 12 13

trigeminal v ganglion

weak

regional

weak expression: see section 02 03 04 05 06 07 08 15 16 17 18 19 20

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 04 16 17 18 19

spinal cord

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15

brain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 05 06 16 17

trigeminal v nerve

weak

regional

weak expression: see section 08 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8405
Entity Detected:	Abcb9, ( MGI:1861729)
Sequence:	sense strand is shown >T8405 CGGCTCCGAGCAAAGCGGTGCCCAGAGCGGGCTCGGTGAGAGGCAGGATCCTGTGTCCCCGCGTCCCGGC CACGGTGTCACCCTCTGACGACTCACCTGGTGGCAGGTGTGGCCCTGCCCAGCTGTCAGTGATGGCCTTG CCCCGGTGAGCCAAAGCCTGCCCCTCTCACCCGCCGGCCACCAGCCAGCAGGATGAGGCTGTGGAAGGCG GTAGTAGTGACGCTGGCCTTCGTGAGCACGGACGTCGGGGTGACCACGGCCATCTACGCCTTCAGCCACC TGGACCGTAGCCTGCTGGAGGACATCCGACACTTTAACATCTTCGACTCGGTGCTGGACCTCTGGGCTGC CTGCCTGTACCGCAGCTGCCTGCTGCTGGGGGCTACCATAGGCGTGGCCAAGAACAGCGCTCTGGGGCCA AGGCGCCTGCGGGCTTCGTGGCTCGTCATCACCCTCGTGTGCCTCTTTGTGGGCATCTATGCCATGGCCA AACTGCTACTCTTCTCAGAAGTGCGCAGGCCCATCCGGGACCCATGGTTCTGGGCGCTCTTCGTGTGGAC CTACATCTCGCTGGCTGCCTCCTTCCTGCTGTGGGGGCTGCTGGCCACTGTGCGGCCGGACGCAGAGGCC CTGGAGCCAGGGAATGAGGGCTTCCACGGGGAGGGTGGGGCCCCTGCTGAGCAGGCATCGGGAGCCACAC TACAGAAGCTGTTGTCCTACACAAAGCCTGACGTGGCCTTCCTAGTAGCTGCCTCCTTTTTCCTCATCGT AGCAGCTCTGGGTGAGACCTTCCTGCCCTACTACACTGGCCGGGCCATTGACAGCATTGTGATCCAGAAA AGCATGGATCAGTTCACCACAGCCGTTGTCGTCGTTTGCCTGCTGGCCATCGGCAGCTCATTGGCCGCAG GTATTCGGGGCGGTATTTTCACCCTCGTATTTGCCAGACTGAACATTCGCCTCCGGAACTGTCTCTTCCG CTCACTGGTGTCACAGGAGACAAGCTTCTTTGATGAGAACCGCACAGGGGACCTCATCTCCCGCCTCACC TCTGATACCACCATGGTCAGCGACCTGGTCTCCCAGAACATCAACATCTTCCTGAGGAACACGGTCAAAG TCACGGGCGTGGTGGTCTTCATGTTCAGCCTCTCCTGGCAGCTTTCCCTGGTCACCTTCATGGGCTTCCC TATCATCATGATGGTGTCCAACATCTACGGCAAGTACTACAAGAGGCTCTCCAAGGAGGTCCAGAGCGCC CTGGCCAGAGCCAGCACCACAGCCGAGGAAACCATCAGCGCGATGAAGACGGTGCGCAGCTTTGCCAACG AGGAGGAGGAGGCCGAGGTGTTCCTGCGGAAGCTTCAGCAGGTGTACAAGCTCAACAGGAAGGAAGCAGC GGCCTACATGTCCTACGTCTGGGGCAGCGGGCTCACACTCCTGGTGGTCCAGGTCAGTATTCTCTACTAC GGGGGCCACCTAGTCATCTCGGGGCAGATGAGCAGCGGCAACCTCATCGCCTTCATCATCTACGAATTTG TCCTGGGAGACTGCATGGAGTCCGTGGGCTCCGTCTACAGTGGCCTGATGCAGGGAGTGGGGGCCGCCGA GAAGGTGTTCGAGTTCATCGACCGGCAGCCCACCATGGTGCATGACGGAAGCTTGGCCCCCGACCACCTC GAGGGCAGGGTGGACTTTGAGAATGTGACCTTCACCTACCGCACTCGGCCCCACACACAGGTCCTACAGA ATGTCTCCTTCAGCCTGTCCCCCGGCAAGGTGACAGCTCTGGTGGGGCCGTCGGGCAGCGGGAAGAGCTC GTGTGTGAACATCCTGGAGAACTTCTACCCTCTGCAGGGCGGCCGTGTATTGTTGGATGGCAAGCCCATT GGCGCCTATGACCACAAGTACCTGCATCGTGTGATCTCACTGGTGAGCCAGGAGCCCGTGCTGTTCGCCC GCTCCATCACAGACAACATCTCCTACGGCCTGCCTACCGTGCCCTTCGAGATGGTGGTGGAGGCTGCACA GAAGGCCAATGCCCATGGCTTCATCATGGAACTGCAGGACGGGTACAGCACAGAGACCGGGGAGAAGGGA GCCCAGCTGTCAGGTGGGCAGAAGCAGCGGGTGGCCATGGCCCGAGCACTTGTGCGGAACCCACCTGTGC TTATCCTGGACGAAGCCACCAGTGCCCTGGATGCCGAGAGCGAATACCTGATTCAGCAGGCCATCCACGG CAACCTGCAGAGACACACGGTGCTCATCATTGCACACCGGCTGAGCACCGTGGAGCGGGCACACCTCATC GTGGTGCTGGACAAGGGCCGGGTGGTACAGCAGGGCACACACCAGCAGCTGTTGGCACAGGGCGGCCTCT ATGCCAAGCTGGTGCAGCGTCAGATGTTGGGGCTCGAGCACCCCTTGGACTACACAGCCAGCCACAAGGA GCCACCCAGCAACACTGAACACAAGGCCTGACAGTGGCCCCCAGGCTGAAGCACTGGAGGCGACCTGCCA AGTCCCACTCCTGCTTCCTGCATCTTGCCTCCTTCCACTTGCTCCCCAGGCACCCGCCAGCTGCCATGGG CTTACCCCCTTCTACTGTGTCCTTCTTGGCTCCTCCAGGCCTGCCAAGGTAGAGCAGCCTTTTTAAACCA AGCTACCAGGGTTTTTACTGCTCAGTTCAACGCACTCCAGTCAACTCTGAGATTTCAAAAAGAACCTTTT TGGGACTGGCAGTGAGCAAGCTCACTAAGATGTTTCCAAGACTTCTGAGCCAAGAGTGCAGGACCCTTTC TGACTGCCTGGAGGATGTCTGGGAGAGAACTAAGCACCACCCTTTGCCTCTCGGGAGAAGCAGCCTCCCT GTCCCCTAGGGGGACACAAGGGTTGGCCTTTCCATCCTTCCAGTCTCCCGGCTCTGTGAGTCAGGCCAGA GGTGGGGCAAGAGAGTGGGAGGTGGCCCACTGCCCATCACCCCCCTGCCACTCAAAGCCAGTCTCACTGT GAACCACTGGGACCTCAGCAGAGGAGGGGCTGGCCGGGCCTGGTGGGCCTCGGGGTGCCCTCAGCCCCGC ACCCAGCACTTGAACCCTTCACCCTCTCCCCTGGCTGGCACCTTCCCTCCTGTCCACCCCTGCAACTCTG CGGAGGAACCTGTCGTTTGTAAGATGTATTCTCAGATTGGTGGAGGGGGACCAAGGCAATGCCCAGGGTC TGGCTTTGTCCTGGTTTCAAGATTTTGGGAGAACTGGGTCAATAAAGTGTACTACCTCTGACCTCTAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686698 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686698 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_008420 same experiment
	EMAGE:29956 same embryo
	EMAGE:30002 same embryo
	EMAGE:30144 same embryo
	EMAGE:29955 same embryo
	EMAGE:29965 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source