EMAGE mouse gene expression spatial database

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EMAGE:30885

2700029E10Rik ( MGI:1919799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004294_01	euxassay_004294_02	euxassay_004294_03	euxassay_004294_04	euxassay_004294_05

euxassay_004294_06	euxassay_004294_07	euxassay_004294_08	euxassay_004294_09	euxassay_004294_10

euxassay_004294_11	euxassay_004294_12	euxassay_004294_13	euxassay_004294_14	euxassay_004294_15

euxassay_004294_16	euxassay_004294_17	euxassay_004294_18	euxassay_004294_19	euxassay_004294_20

euxassay_004294_21	euxassay_004294_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8259
Entity Detected:	2700029E10Rik, ( MGI:1919799)
Sequence:	sense strand is shown >T8259 GCAGCACCACTCACCCCCTTGTCCCAGCTCCTGAGTGAGCGTCCCTCCGCTTGTCTCTTGGGGTCATCCC CCTAGGCTGTCAGGATGGTGTCCTGGATGATCTGTCGCCTGGTAGTGCTCATATTTGGCATGCTGTATCC TGCGTATGCTTCCTACAAGGCCGTGAAGAGCAAGAACATTCGAGAATATGTACGGTGGATGATGTATTGG ATTGTCTTTGCGATCTTCATGGCAGCAGAAACCTTCACAGACATCTTCATTTCCTGGTTCCCGTTTTATT ACGAGTTCAAGATGGCTTTTGTGCTGTGGCTGCTCTCACCTTACACCAAGGGGGCCAGCCTGCTTTACCG AAAGTTTGTCCACCCATCCCTATCCCGCCATGAGAAGGAGATCGACGCGTGTATCGTGCAGGCAAAGGAG CGCAGCTATGAAACCATGCTCAGTTTTGGGAAGCGGAGCCTCAACATCGCTGCCTCAGCTGCTGTGCAGG CTGCTACCAAGAGTCAAGGCGCTCTAGCTGGAAGGCTGCGGAGTTTCTCTATGCAAGACCTGCGCTCTAT CCCTGACACCCCTGTCCCCACCTACCAAGATCCCCTCTACCTGGAAGACCAGGTACCCCGACGTAGACCC CCTATTGGATACCGGCCAGGCGGCCTGCAGGGCAGTGACACAGAGGATGAGTGTTGGTCAGACAATGAGA TCGTCCCCCAGCCACCTGTTCGGCCCCGAGAGAAGCCTCTAGGCCGCAGCCAGAGCCTTCGGGTGGTCAA GAGGAAGCCATTGACTCGAGAGGGCACCTCACGCTCCCTGAAGGTCCGAACCCGGAAAAAGGCCATGCCC TCAGACATGGACAGCTAGAGTCTGCAGATTGAGGCCACCTTACCTCTGGAGCCAGCAGGGGACCTTTCGC TGCTACACCAGCTACCGGGGTTCTGCTCCGTCTGGCTTGTGCCTAAATGGCACATGGCGTGGTACCCTGC ACAGGGAGACATTCACTATACCAAAGCAGCCCAGGCCTGGGGCCTATTTATTGCCTTCCTCTGCCTTTTA CTTTCTCAGACACGGGACCAGAGCCCCACCAGTCCCTACCGACGAAACCAAAAGTCCAACCAGCTGTGTT CATTCCTTCTTGTCCTTCAAAATACTTGACAGCCTTTTCCAAGGCCTGGTGTGTGTGTGTGTGTGTGTGT GTGTGTGTGTTTACGTACACTAGCTGCATGTTTCGTGTTGGTGAGTGAGGTCAGGCTTATGAATATTTTT ATATAAATAAATACCAAACAGTGTTTGGCCTCTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1363539 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1363539 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004294 same experiment
	EMAGE:29923 same embryo
	EMAGE:32210 same embryo
	EMAGE:29925 same embryo
	EMAGE:29535 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source