EMAGE mouse gene expression spatial database

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EMAGE:30914

Tmem18 transmembrane protein 18 ( MGI:2387176)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003177_01	euxassay_003177_02	euxassay_003177_03	euxassay_003177_04	euxassay_003177_05

euxassay_003177_06	euxassay_003177_07	euxassay_003177_08	euxassay_003177_09	euxassay_003177_10

euxassay_003177_11	euxassay_003177_12	euxassay_003177_13	euxassay_003177_14	euxassay_003177_15

euxassay_003177_16	euxassay_003177_17	euxassay_003177_18	euxassay_003177_19	euxassay_003177_20

euxassay_003177_21	euxassay_003177_22	euxassay_003177_23	euxassay_003177_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 08 09 10 11 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4918
Entity Detected:	Tmem18, transmembrane protein 18 ( MGI:2387176)
Sequence:	sense strand is shown >T4918 GCGGACTCGTCCCGGCTGTTGGGACCCCTGGCGCGATGTCGTCCGCATACTCAGTCCGATCCTTCCCAGT CAGCATCCCTGCGGTGATCATGGAGACAGACTGGACAGAGCCGTGGCTCCTAGGACTCCTTGCCTTCCAC CTGCTGTGCCTCCTTCTCACTTGCTTCTCCTCACAGAGGTATAAGCTACAGATCGGGCACTTCCTGTGTC TGGTAGTGTTAGTCTATAGTGCCGAGTACATCAACGAAGTGGCTGCAGTGAACTGGAGATTGTTCTCAAA GTACCAGTACTTTGACTCCCGGGGGATGTTCATTTCTCTTGTGTTCTCAGCGCCACTGCTCTTCAACGCC ATGCTCATTGTGATTATGTGGGTACGGAAGACTTTGACTGTGATGACCGACCTGAAGACCTTACAAGAGG AAAGAAAGGAGAGGAGGAGGAGGAGGAAGGAAGAGTAATGGTGAACTGCTGCTGTGCGCCATGTTTGTCC TCTGACTTGCTTGTTTCCAGCTGGGAACTAACTGCACATGGCCCAGATGCCAAATGTCAGCCGACTCATG GTTTAGAAAAAGGTCCTTATAGCTTAGCACACCACAACTGGTTCTTATAGTTTACTCTGTTTGGAGCCCT CTTAAAGCTGGTTCTTAAAAGAACTGATGGCATCACCGAAACACGTTGGATGGGGACCCCTCCTTTACAT GGGGAGAGCTTCCTGGTTCAGCCACTTTATTCACAGCTTCTCACACGTTGCTGGTAGACCTCATTATGTA ACTAGAGTTCACGAGGTTCTAAGTGTGGACACTGGGAAATAAAGAGCCCCGATCCTGCTGGGATCACTAC CTGCTCCCTCTTCTGTTTCTAGCCTGTAGCGGTGGCTATCAGCTAAGGGTGGCTTTGTTCCCCAGGAACT TTGCTGATGCTTGTCACCGCAGGTGGACATGGTACTTGTGTCCATAGGACAGAGCCGAGTCCTGCAGGGC ACAGGACAGCCCCACCGTGGAGTATTGCTGTGTACATAATGTCAGCAGTACCCAGGCTGAGGGAGCGGTG GGTCAGGTCTGGGGAAGAATGCGGTGTGTTTCAGTTCCCATGAAGCTAGGCCTCTAAATAATTGAGTTGG GGCTATGCCACTGGGAATGTTTTGTTTAAAAATATTAAGCTAGAATGAAAGCTGTAAAGTCAAGACTCCG TGGAAAATAGTATGTCAAATGAACCGTTTGGTCTTGTTTTTTCTGGATACAGAATTATAAATACCTGCAT GCTCTGTGAATGGCTTATCTGTTTCTACCCAGACTGTGACAAGTGCAGACAGTTCTTCCCAAGTGTCACA TTTGTGCGGTTGGGGGCTGGCGAACACAGCAGGGTGTATTGTCACCTGTGCCTCTGTTTTGTCAGACACC CTTGGTTTCCATGGAATCCGGAGCAATAAACCTTTAGAGATTAGAGGTGACAGAGACCCAGACTTAGAGA CAGTAAGAAATACACTCTTGGCTTTAGTTTAAAGAAATAATTTGTGAAGTATTTTCTTTATTTAATAGGA TTTTTTCCTTTCTAAACATTCTTCAAAGTTCTAAGCTAAAATAAAATCTAGTTAAAAATATTTAAAAGGT TAAAAGTAGATCGTTTGATAGACTACCGTTATGTTCTGAAACCCAGTTATATAGTTTCTAATGTTTATCC AGCTTCCGGATTTAACAGAAACCTACTCACTGTCTTGTGACAATCTGATCATCCTGTGGTGTGAGTTTGC GCCTCCTGCTGCAGCACTTAACCTGCATTGTGCACAGTCTCTTGATTTGTTCCACTTCACATTGAACAAA AAAGTGCCAAGGAAAATAACACAGTGTTAAGTTCAGATTTTGAATTTAAATCACACTGTTAAGTCGTGGA CATTGTGTCTAAAGGGATACCTAATTGTATGTTTCTGAAGTTTTCTGGTTCAGGATATTTGATCATTTTA TGGTTACAAAGGTGCTTACCATAACATTGATTAGTTTCATAGAAAATAAGAACAAATTAGTCCGTTAGAT AAATCTGAAACAACTGAGTGTCAGATAGGCCTGGACTCACCAACTAGATGTGTTCCCTTCAGGCCTTTGT GTCTGAGGCCAGTGGGGCAGAATGGGGCAGAGAGCGGCTGCGATGTCCGCGTTCTGGCATATGCCGGTAT TAATACCTCAGCTTGTGTAACCATGACCCTGACCGGCTTTCTTGCCTTGTTTCTGCACGTGTGGTTGTGG ATGCACACGTGCTTCGTTTTTTCCATAATTAAATGTTTATGTTTTGTTTTGGATTAGAGAGATGGCTTCG TTGGCACTCTCCACCTGAGTTTGGTCTCCAGAGGAACTTAACATTTGTTCCCAAGCATTACACGCTAAGT CCCATTGCTGGGAAAACCTGCCTGATGGAAGGACGGAAGCGGCCACTACAGGTTGTCCTCTAACTGTATG TGCACACAAAATAAAGTCATGCACGAGTCACTCCCGACCAACAGCACTTGAGGTGGCCCTCTGGCTTTCA CACACACATGTGTGCACACTGCTACACACATAGCATCTACACAAATGTTGTGTGTTTTCTGACATTCACT CTGTGACTAAAATTATACTTGGAGGAACTTTTAAACATTTTGCCCTGATATTAAGGAAAATCCTGCAAGG TGCACCTATGAACAAGCAGTTGGGTTCTGTGCTGCTTTGGGGATTTTACGGGTTTGCCCAGTGCTGTGTT CACACTTCCGTAAAGGAAGGTTTACAGGCACTGATGGACACAGTGAGCACTGAGTTTACTGAGTAGTTGT GGAAAGCTCTGGAAAGCCCTAAAATAACCATCTTATACATTCCAGTCTATGAAACTTCATGTTTGTTGAT TATTATGCATTAATATACAATAAACTATGTTTGATAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5116657 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5116657 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003177 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source