EMAGE mouse gene expression spatial database

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EMAGE:30921

Cldn11 ( MGI:106925)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006682_01	euxassay_006682_02	euxassay_006682_03	euxassay_006682_04	euxassay_006682_05

euxassay_006682_06	euxassay_006682_07	euxassay_006682_08	euxassay_006682_09	euxassay_006682_10

euxassay_006682_11	euxassay_006682_12	euxassay_006682_13	euxassay_006682_14	euxassay_006682_15

euxassay_006682_16	euxassay_006682_17	euxassay_006682_18	euxassay_006682_19	euxassay_006682_20

euxassay_006682_21	euxassay_006682_22	euxassay_006682_23	euxassay_006682_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pectoralis minor

strong

regional

strong expression: see section 08 09 10 11 12 15 16 moderate expression: see section 07 14 17 18 19 20

mandible

moderate

regional

moderate expression: see section 10 13 19 20 weak expression: see section 05 07 08 09 17 18 21

pectoralis major

strong

regional

strong expression: see section 08 09 10 11 12 15 16 moderate expression: see section 07 14 17 18 19 20

temporal bone petrous part

strong

regional

strong expression: see section 22 23 24 moderate expression: see section 01 02 03 05 06 20 21

midbrain meninges

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

kidney calyx

moderate

regional

moderate expression: see section 07 08 17 18

hindlimb digit 4 mesenchyme

strong

regional

strong expression: see section 20 moderate expression: see section 01

diencephalon meninges

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

foot mesenchyme

strong

regional

strong expression: see section 18 19 moderate expression: see section 02 03

telencephalon meninges

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

nose

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

ureter

moderate

regional

moderate expression: see section 09 10 14 15 16

hindlimb digit 2 mesenchyme

strong

regional

strong expression: see section 20 moderate expression: see section 01 21

hindbrain meninges

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21

meckel's cartilage

moderate

regional

moderate expression: see section 08 09 10 13 17 18 19 weak expression: see section 07

trachea

strong

regional

strong expression: see section 13 15

hand mesenchyme

moderate

regional

moderate expression: see section 01 02 23 24

hindlimb digit 3 mesenchyme

strong

regional

strong expression: see section 20 moderate expression: see section 01 21

posterior naris

strong

regional

strong expression: see section 13 weak expression: see section 17

spinal cord meninges

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19

maxilla

moderate

regional

moderate expression: see section 20 21 weak expression: see section 04

adrenal cortex

moderate

regional

moderate expression: see section 07 08 09 16 17 18

esophagus epithelium

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7328
Entity Detected:	Cldn11, ( MGI:106925)
Sequence:	sense strand is shown >T7328 CCACGCGTCCGCGCTGCACGCTGTCGCAGCAGTGCTCGCAGCCGCTCTCTCCCCATCTCGAGTAGCCCGG AGCCAGCGGCTCGCGAGGGCCAAGAGGGCAAGCCTAGGGAAGGCTCTGTCCAGGACGACACAGGGGGCAC AATCCGTGTGAGTCGAGCTGCGTGGACGTCGCTGCGGCCACCATGGTAGCCACTTGCCTTCAGGTGGTGG GTTTCGTCACGAGCTTCGTGGGTTGGATTGGCATCATCGTCACAACGTCCACCAATGACTGGGTGGTGAC CTGCAGCTACACCATCCCCACCTGCCGAAAAATGGACGAACTGGGCTCCAAGGGCCTGTGGGCTGACTGC GTCATGGCCACTGGTCTCTACCACTGCAAACCCCTGGTGGACATCCTCATCCTTCCAGGCTACGTGCAGG CTTGTAGAGCCCTCATGATTGCTGCCTCCGTTCTGGGCCTGCCCGCCATCTTGCTGCTGTTGACAGTTCT CCCCTGCATCCGAATGGGCCACGAGCCTGGAGTGGCCAAGTACAGGCGAGCCCAGCTGGCTGGGGTGCTC CTTATTCTGCTGGCTCTCTGCGCCATTGTCGCCACCATCTGGTTTCCTGTATGTGCCCACCGCGAGATCA CCATCGTGAGCTTTGGCTACTCGCTGTACGCAGGTTGGATCGGTGCTGTGATGTGCCTGGTGGGTGGCTG TGTCATCGTCTGCTGCTCCGGGGATGCACAGTCATTTGGAGAAAACCGTTTCTATTACTCTTCTGGTTCC AGCTCGCCAACGCATGCCAAGAGTGCCCATGTCTAAGAGGGCTGCTCCACTGCCCGCCGAGGTGCTGTAA ATGCTGGGCCTGGGCCCTGGGTTTGCTCGCCACAGTGGGGAGAAGCCCACTTCCCTGCCAGGCACTAAAG CCAAAGTTCTAGAAAGTATCCTGCCCCGGCATTTTGAAGTCGTAACACCCCACCCACCCACCCACCACTT CTTGGCTGCCTTAAAAGAAAGCTCTAGCTCAGTTAATGCCCACATAGTTTTCTCCTGGAGTTGCGGGCTG TGGCTGTTTGCTCTTTCCTCGGGCATTCCATTGTTGTTGATTAAAAAAATATTTTGTTTCTCTCTTAAAT TCAAATGTCTTGGGAACATTGCTGACTTGGGTGTGGATTGGGAAAGAAATAAAAGATGCTTTTCAAAGGG TTACCAACGACAGTGGAAGCCTTATAGAGACAGCTCTCTTCTCCCTTTCGGCTTAGTTTCAAGGTCACTT ATATATAAGAGATAGAAATGGATAGATTGGGAACACGGGTGGGAGGGGAACTCAGAGCTTTCCCTCCACG GGAAGCTTCTCTTTTATAAGTTGAGGGGTTGGGTGTCTTTTTTTTTTTTTAGTTTGCGATTTTACATTTT TCTGTACGTACTTTTTCAAGATTGATCATTTTTATAACCACGGGTTTCCTGAAAATTCTCAATTCACCAA TATGAAGGAAATGAACCAAGCAGACGTTAATATGCAATAAATAATAGTACGAAGATTATAACTTTAACTG ACTGCCCACGGTTTCCAGGTTTGTATGCTATAGTTTTTAATCCTATGGTTGCATATGCTTCAAATTAACA CATTTAAAAATCTTTTCTCCCCTTCTATTTCTGTCTCCATTCTGTTAGAGACCATGAAGCAGTATTGTTT AACATAAGTTGTACTGTTAAGTTTGGCTTCATGGGTGTAAACACCAATGGTCTGTCAGTGTCTAAGACTC TGGATACTGCAAGCTCCGTCCGGTGCATTTGTTCAGGTAAAATCTGTGCAATAAAATAACAAACTGTCTC CAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4190031 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4190031 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006682 same experiment
	EMAGE:30391 same embryo
	EMAGE:30329 same embryo
	EMAGE:30318 same embryo
	EMAGE:30948 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source