EMAGE mouse gene expression spatial database

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EMAGE:30922

Sil1 endoplasmic reticulum chaperone SIL1 homolog (S. cerevisiae) ( MGI:1932040)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003174_01	euxassay_003174_02	euxassay_003174_03	euxassay_003174_04	euxassay_003174_05

euxassay_003174_06	euxassay_003174_07	euxassay_003174_08	euxassay_003174_09	euxassay_003174_10

euxassay_003174_11	euxassay_003174_12	euxassay_003174_13	euxassay_003174_14	euxassay_003174_15

euxassay_003174_16	euxassay_003174_17	euxassay_003174_18	euxassay_003174_19	euxassay_003174_20

euxassay_003174_21	euxassay_003174_22	euxassay_003174_23	euxassay_003174_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 11 12 13 14 15 moderate expression: see section 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4200
Entity Detected:	Sil1, endoplasmic reticulum chaperone SIL1 homolog (S. cerevisiae) ( MGI:1932040)
Sequence:	sense strand is shown >T4200 CAGCCTTCGGCAGCCGCTGGACGGCGTTTGCGCACGAGGAGCTGGTTGCCGCTGCAGCCGCCTGGAATTG TGGGGATTGTGTCTGCCCCTCGGCTGCCGTGGAAAGCCGAAGGTCCTGGTTATGGCTCCTCAGCACCTGC CTTCAACTAGGATGGCCTCTCCAGGAATGCTGCTGGGGCTGCTGTTGACTTCCTGTTTAACTCTCTGCCT CAGCTGTCAGAACTCAAATAATTTTGCACTCACCAACCCAGAGAAGAGCATCCACCAGGAATCAGATACA AAGGAGACCAGGGAAGAGGAGGAGCTAGACACCGAGATCCTGGAGGTGTTCCACCCAACTCAAGAGTGGC AGACCCTTCAACCAGGTCAGGCTGTTCCTGCAGGATCCCATGTGCGAATGAACCTACAGACTGGAGTAAA CGAGGTGAAGCTCCAACAAGAAGACAAATTTCAAAATAATCTGAAAGGATTTAAAAGAGGCAGAAGGCTG GACATCAACGCCAACACATACACATCTCAGGACCTCAAGAGTGCGCTGGCCAAATTCAAGGAGGGGACCG AGATGGAGAATTCAAAGGATGAACTGGCGAGACAGGCCACAGTCAAGCAGCTCTTCCGCCCCATTGAGGA GCTGAAGAAGGAATTTGATGAGCTGAACGTTGTTCTTGAGACTGACATGCAGATTATGGTTCGGCTGATA AACAAGTTCAACAGCTCCAGCTCCAGCCTAGAAGAGAAGGTTGCTGCGCTCTTTGATCTTGAGTACTATG TCCATCAGATGGATAACGCACAGGACCTGCTGTCCTTTGGTGGCCTTCAAGTGGTGATTAATGGGCTCAA CAGCACAGAACCCCTGGTGAAGGAGTATGCTGCTTTTGTGCTGGGGGCCGCCTTTTCCAGCAATCCGAAG GTCCAGGTGGAAGCCATCGAAGGGGGAGCCCTGCAGAAGCTGCTGGTCATCCTGGCCACAAACCAGCCTC TCCCCGCCAAGAAGAAGGTCCTGTTTGCACTGTGCTCCCTGCTGCGCCACTTCCCCTATGCCCAGCAGCA GTTCCTGAAGCTCGGGGGCCTGCAGGTCCTCAGGAGCCTGGTACAGGAGAAGAGTGCGAAGGTACTGGCC GTGCGTGTGGTCACCCTGCTCTATGACCTGGTCACTGAGAAGATGTTTGCTGAGGAGGAGGCAGAGTTGA CCCAGGATTCATCCCCAGAGAAGCTGCAGCAGTATCGCCAGGTGCAGCTCCTGCCAGGCCTGCAGGAACA GGGTTGGTGTGAGATCACCGCCCAGCTCCTGGCCCTTCCAGAGCATGATGCTCGGGAGAAGGTGCTGCAG ACACTGGGTGCCCTCCTGACCACCTGCCGGGATCGCTACCGTCAGGACCTCCAGTTGAGCAGGACACTAG GGCGTCTCCAAGCAGAGTACCAAGCCCTGGCCTCCCTGGAGCTCCAAGAAGGCGAAGATGATGGCTACTT CCGGGAACTGCTGGCCTCCATCAACAGCTTGATGAAGGAACTAAGATGAGGCTGCCATGCCGAGACCAGG ACTGGACGGATGCTGCTAGTGAGAGCTTTGGTGGGCTCTTCAGGGGACATCAACTACGAGGAGGACTTAA CCTGCTGGGGCATGAGTGTCACCTGGGCAGAGCTTGCTAGGTCATTAAACAGAGACATGATGACAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4489217 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4489217 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003174 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source