EMAGE mouse gene expression spatial database

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EMAGE:30924

Sephs2 selenophosphate synthetase 2 ( MGI:108388)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002088_01	euxassay_002088_02	euxassay_002088_03	euxassay_002088_04	euxassay_002088_05

euxassay_002088_06	euxassay_002088_07	euxassay_002088_08	euxassay_002088_09	euxassay_002088_10

euxassay_002088_11	euxassay_002088_12	euxassay_002088_13	euxassay_002088_14	euxassay_002088_15

euxassay_002088_16	euxassay_002088_17	euxassay_002088_18	euxassay_002088_19	euxassay_002088_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 07 08 09 10

hindgut

moderate

regional

moderate expression: see section 06 08 09 10

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

foregut-midgut junction

moderate

regional

moderate expression: see section 06 08 09

stomach

moderate

regional

moderate expression: see section 05 06

midgut

moderate

regional

moderate expression: see section 05 06 08 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5470
Entity Detected:	Sephs2, selenophosphate synthetase 2 ( MGI:108388)
Sequence:	sense strand is shown >T5470 GGTCCGGCTCGCGTGGTTGATCATCTCCTGGCGTAACCTTGGCCCGCTGTGGCTGGGAGACTCATCTGCA GGTATCTGGGCCTTCTGGTCCGCACGGCCTCCCGGGCGAGCGGCGCGATGGCGGAAGCGGCGGCGGCGGG CGCCAGCGGAGAAACCATGGCGGCGCTAGTGGCCGCAGAAGGTTCCTTGGGCCCGGCGGGCTGGTCTGCC GGCCGGAGTTTCTCCAACTACCGGCCGTTCGAGCCCCAGACACTGGGCTTCAGCCCGAGCTGGCGGCTGA CGAGCTTCTCCGGCATGAAGGGCTGAGGCTGCAAGGTCCCCCAGGAGACCCTGCTCAAACTCCTGGAGGG ACTGACGCGGCCCGCGCTGCAGCCCCCGCTTACCTCGGGTCTGGTCGGGGGCCAGGAAGAGACGGTGCAG GAAGGGGGCCTGTCCACCAGGCCCGGCCCCGGCTCAGCCTTCCCCTCGCTGAGCATTGGCATGGACTCCT GCGTCATCCCCCTGAGGCACGGAGGCCTGTCGCTGGTGCAGACCACCGACTTCTTTTACCCCTTGGTGGA AGATCCCTATATGATGGGGCGCATAGCTTGTGCCAATGTGCTCAGTGACCTCTATGCCATGGGTATCACT GAGTGTGACAACATGTTGATGTTACTCAGTGTGAGCCAGAGCATGAGTGAAAAGGAACGAGAGAAGGTGA CACCGCTCATGATCAAAGGCTTTCGTGACGCTGCGGAGGAGGGAGGCACTGCAGTGACCGGTGGACAGAC AGTGGTCAACCCGTGGATTATCATCGGTGGCGTTGCCACGGTGGTGTGTCAGCAAAATGAATTCATAATG CCTGATAGTGCCGTGGTAGGAGATGTGCTGGTATTAACCAAACCTTTAGGAACCCAGGTTGCTGCCAATG CCCACCAATGGCTGGATAATCCTGAGAAATGGAATAAAATCAAGATGGTGGTTTCCAGAGAGGAAGTAGA GTTAGCCTATCAGGAAGCTATGTTCAATATGGCTACTCTAAACAGGACTGCTGCTGGCTTGATGCACACT TTTAATGCCCACGCAGCCACGGATATCACAGGCTTTGGCATATTAGGACACTCTCAGAACCTGGCAAAAC AGCAAAAAAATGAAGTGTCCTTTGTCATACATAATCTGCCAATAATTGCCAAGATGGCTGCGATCAGCAA AGCCAGTGGGCGCTTTGGCCTCCTCCAAGGAACGTCAGCTGAAACCTCTGGGGGATTACTGATTTGTCTG CCAAGAGAGCAGGCGGCCCGCTTTTGTTCGGAAATCAAATCTTCCAAGTACGGAGAGGGTCACCAAGCTT GGATCGTTGGCATCGTGGAGAAGGGAAACCGGACAGCCCGGATCATTGACAAGCCTCGCGTTATTGAAGT TCTACCTCGGGGAGCCTCTGCTGCTGCTGCTGCTGCTCCTGATAATTCCAACGCAGCCTCTGAGCCTAGT TCTTGAAATGGAATAGCGGTTGTTGGGAACTCGGAGCCATTCTACCCGCTCAGGGACTGCTGGCCAGGGT TGATTTTAAGACCTTTCCAAAGGCTGCTTGCATGGTTCCTCCAGGCCCATCCAAAGCTTCCTGTATGTGC ATCCAGGCCTGTGAGTAATGGCGCTGCGGATGTGTGTTCATCTGTTGAGAGCATGAGGAGCAAAAACCCG TTTCCCAAAGCAAGAGGAGGCTATTTCAGTTTTAGGGATTTTTTTTTTTTTTTTTTTTTGCACTGAGTTG ATTCATTTCTGCACAGGGAGTAAAGATTATTAAGATTACATATGAGAAAAGTAAACCTGCAACATGAAAA ATTATTTGGACCAATATATTGATAAATCTAAATTGTTAGGAGAACTCTTACTGATTTATTGTCAAATTTG TTATTAATTTTTTTCTGAGAAACTGCCTCTTTTCCTGTTCTGGACAAGAGTTGAGCAGCTTGTCCGACAG GAAAGGAAGACTAGCCACCTGACTTGGTCTCTGATAATGATGTCTCTCCCTCTAACTCCCAGTAAGGACT GGGAGAGGCTGAACAAACCTCAGAGCCAGGTGTCGGTGGCCATTGAATCTTACACTGAAACTTCTGGAGA TTTAATTAATAAAGAGAATTTCTTACAGTAACTAAATAAAAGGGACTTTGTTGGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5374184 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5374184 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002088 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source