EMAGE mouse gene expression spatial database

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EMAGE:30926

Sssca1 Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) ( MGI:1913482)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003176_01	euxassay_003176_02	euxassay_003176_03	euxassay_003176_04	euxassay_003176_05

euxassay_003176_06	euxassay_003176_07	euxassay_003176_08	euxassay_003176_09	euxassay_003176_10

euxassay_003176_11	euxassay_003176_12	euxassay_003176_13	euxassay_003176_14	euxassay_003176_15

euxassay_003176_16	euxassay_003176_17	euxassay_003176_18	euxassay_003176_19	euxassay_003176_20

euxassay_003176_21	euxassay_003176_22	euxassay_003176_23	euxassay_003176_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 17 18 19 20 21

lower jaw incisor

weak

regional

weak expression: see section 12 13 15 16 17

upper jaw incisor

weak

regional

weak expression: see section 11 12 13 16 17

axial musculature

weak

regional

weak expression: see section 06 07 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4917
Entity Detected:	Sssca1, Sjogren's syndrome/scleroderma autoantigen 1 homolog (human) ( MGI:1913482)
Sequence:	sense strand is shown >T4917 ACGGCAACATGGCCTTGAACGGTGCAGATGTCGACGATTTCACCTGGGAGCCTCCGACTGAAGCTGAGAC GAAGGTGCTGCAGGCGCGACGGGAGCGGCAGGATCGCATCTCCCGTCTGATGGGGGACTACCTGCTCCGT GGTTACCGCATGCTGGGCGACACGTGCGCCGACTGCGGGACGATCCTCCTGCAAGATAAACAGCGGAAAA TCTACTGTGTGGCTTGTCAGGAGCTCGACTCAGACGTGGATAAAGATAACCCGGCACTGAATGCACAGGC GGCGCTCTCTCAAGCTCGGGAACACCAGCTGGCCTCATCTACAGAGCCTGCCTCGAGCTCTCGGCCCCCA TCCCAGCCACCAGTACCCCGTCCAGAGCACTGTGAAGGCGCAGCAGCGGGGCTCAAGGCAGCCCAGGCGC CTCCTCTTCCGGCTGCACCTCCAAATACGGACGCGGTGGCTTCCACACAGACGGCCCTCCTGCAGAAGCT AACCTGGGCGTCAGTCGAGCTGGGCTCTAGCACCTCCTTGGAGACTAGTATCCAGCTCTGTGGGCTTATC CGGGCTTGCGCCGAGGCCCTAGGCAGCCTGAAACAGCTGGATCACTGAGACCTCTATCCTGATCCCTGTT CCCCTGAGAAAAACCCTCCAGAAAAACAGTTTTTCCTCCGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5102521 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5102521 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003176 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source