EMAGE mouse gene expression spatial database

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EMAGE:30948

Cdkn2d cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) ( MGI:105387)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006695_01	euxassay_006695_02	euxassay_006695_03	euxassay_006695_04	euxassay_006695_05

euxassay_006695_06	euxassay_006695_07	euxassay_006695_08	euxassay_006695_09	euxassay_006695_10

euxassay_006695_11	euxassay_006695_12	euxassay_006695_13	euxassay_006695_14	euxassay_006695_15

euxassay_006695_16	euxassay_006695_17	euxassay_006695_18	euxassay_006695_19	euxassay_006695_20

euxassay_006695_21	euxassay_006695_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 14 15 16 17

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 16

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 09 10 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 17 18 19 20 21

telencephalon mantle layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 21

liver left lobe

strong

spotted

strong expression: see section 01 02 03 04 05 moderate expression: see section 06 07 08 09 10

liver right lobe

moderate

spotted

moderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 08 17 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 18

trigeminal v nerve

moderate

regional

moderate expression: see section 09 17

pons mantle layer

weak

regional

weak expression: see section 07 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7135
Entity Detected:	Cdkn2d, cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) ( MGI:105387)
Sequence:	sense strand is shown >T7135 CCACGCGTCCGCAGCAAGAGCTGGTTCCCCAGCCCTGACCTTAACTGGGCTTGGGGCTGGGTGGGTTTCA CAGTCCACCGGTATCCACTATGCTTCTGGAAGAAGTCTGCGTCGGCGACCGGTTGAGTGGCGCAGCGGCC CGTGGCGACGTGCAAGAGGTCCGCCGCCTTCTTCATCGGGAGCTGGTGCATCCTGACGCCCTGAACCGCT TTGGCAAGACGGCCTTGCAGGTCATGATGTTTGGAAGTCCAGCAGTTGCTTTGGAGCTCCTGAAGCAAGG TGCCAGCCCCAATGTCCAAGATGCCTCCGGTACTAGTCCTGTGCATGATGCGGCTCGCACCGGGTTCCTG GACACCCTGAAGGTTCTGGTGGAGCATGGTGCTGATGTCAATGCCCTGGACAGCACTGGGTCGCTCCCCA TCCATCTGGCGATAAGAGAGGGCCATAGCTCCGTGGTCAGCTTCCTAGCTCCTGAATCTGATCTCCACCA CAGGGACGCTTCCGGTCTCACTCCCCTGGAGTTGGCTCGGCAGAGAGGGGCTCAGAACCTCATGGACATT CTGCAGGGCCACATGATGATCCCAATGTGACCCAAGGCCACTGTCTCCAGCCTTACTGGGTTACTTGTCA ACAAAAGAGGAAAGAAACTTTCTCTTTTCACACCTGTCCATTGAAGAAGGGAGTGGGAGGAGCAGTTTGT GGTTTATTGGTGTTGATTTCTTGAGTGTGTGTGTTTGGGGGGTGTTTCTCATTTGTTTTTCTCACCCCTT TTGGTGTGTTGGACAAAGAAGGGGCTCCTACAGGCAACAGCCATCTAAACGGTTCAGTTTCCTCTGCACT GGGGCTGCACCAGGGCAGGGGTTTAAAGCCCTAGCCTCAGAGTGAGGTCATCACTTCCCGGGCCCCTTGG AAGCTGGTGACCTTGGCAGGCTGTGCTCAGAGAGCCCTGAAGTGTGAGCCATCTACTTTGGGCATGTGAG GGGGAGGGGAAAACATTTCAAATCAATTAAAAGGATAACATGAGTTCATTTTTCTTTCTTGGAAGGTTTC CAGTCTGTTGTACAGAGTTTGAAATATAAATATTTATTGCTTTATGGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4020283 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4020283 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006695 same experiment
	EMAGE:30391 same embryo
	EMAGE:30329 same embryo
	EMAGE:30318 same embryo
	EMAGE:30921 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source