EMAGE mouse gene expression spatial database

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EMAGE:30950

Echs1 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial ( MGI:2136460)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001897_01	euxassay_001897_02	euxassay_001897_03	euxassay_001897_04	euxassay_001897_05

euxassay_001897_06	euxassay_001897_07	euxassay_001897_08	euxassay_001897_09	euxassay_001897_10

euxassay_001897_11	euxassay_001897_12	euxassay_001897_13	euxassay_001897_14	euxassay_001897_15

euxassay_001897_16	euxassay_001897_17	euxassay_001897_18	euxassay_001897_19	euxassay_001897_20

euxassay_001897_21	euxassay_001897_22	euxassay_001897_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 03 04 05 10 11

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T658
Entity Detected:	Echs1, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial ( MGI:2136460)
Sequence:	sense strand is shown >T658 CTGGATGTGATATCATCTATGCTGGCGAGAAAGCCCAGTTCGGTGGCCAGAAATCCTCCTGGGGACCATC CCAGGTGCTGGAGGCACTCAGAGACTCACCCGAGCAGTCGGCAAATCGCTAGCAATGGAGATGGTCCTCA CTGGTGACCGCATCTCAGCTCAGGATGCAAAGCAGGCAGGTCTTGTAAGCAAGATTTTTCCTGTTGAAAA ACTGGTTGAAGAAGCCATCCAATGTGCAGAAAAAATTGCCAGCAATTCTAAAATCGTAGTAGCCATGGCG AAAGAATCTGTGAATGCAGCCTTTGAGATGACGTTAACAGAAGGAAATAAGCTGGAGAAGAGGCTTTTCT ATTCCACCTTTGCCACCGATGACCGGAGAGGAGGGATGACTGCATTTGTAGAGAAAAGGAAGGCCAACTT CAANGACCACTGAGAACTGGCAGCTATACCGTTTGCCACCCTGGAAAGCTCAGCCTGTCCTTTGAGAGGC AAATAAATGATCCAAAAGGGTAGTAGTGTCGGCCACAGT
Notes:	The probe template was PCR amplified from IMAGE:1887728 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1887728 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001897 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source