EMAGE mouse gene expression spatial database

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EMAGE:30954

Aadat ( MGI:1345167)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002742_01	euxassay_002742_02	euxassay_002742_03	euxassay_002742_04	euxassay_002742_05

euxassay_002742_06	euxassay_002742_07	euxassay_002742_08	euxassay_002742_09	euxassay_002742_10

euxassay_002742_11	euxassay_002742_12	euxassay_002742_13	euxassay_002742_14	euxassay_002742_15

euxassay_002742_16	euxassay_002742_17	euxassay_002742_18	euxassay_002742_19	euxassay_002742_20

euxassay_002742_21	euxassay_002742_22	euxassay_002742_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

liver lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

kidney calyx

strong

regional

strong expression: see section 09 10 11 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T983
Entity Detected:	Aadat, ( MGI:1345167)
Sequence:	sense strand is shown >T983 TCTCGAGNCTGTTGGCCTACTGGTATTTAGATCTCAGACACCTACCGCAGCCGGTAGGCAGGCAGATTGG GTGGCACTACACAGGGACAGCTAACAGTGCATCCCGAGTGACTGGCTTTCTGAATCCTGCTCCACGCGAC CAGCAGAGACATGAATTACTCACGGTTCCTCACTGCAACGAGCCTGGCCAGAAAGCCATCTCCCATCAGA ACTACAGCGGACATACTGAGCAAAGCACCAAAAACCCTCATCTCCCTGGCTCCTGGATCTCCAAACCCGA GCATGTTCCCCTTTAAGTCAGCTGCCTTCACTGTGGAAAACGGAAGCACCATCCGGTTTGAAGACGACTT GATCAAAAGGGCCCTCCAATACTCTCCAAGCTATGGAATTCCAGAACTTCTGTCCTGGCTAAAACAGTTT CAAGTAAAATTGCATAATCCCCCCACTGTCAACTACCCACCCAATCAAGGACAGATGGATCTCTGCATCA CATCTGGCTGCCAAGATGGTCTCTGTAAGGCATTTGAAATGCTCATCAATCCTGG
Notes:	The probe template was PCR amplified from IMAGE:1972515 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972515 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002742 same experiment
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source