EMAGE mouse gene expression spatial database

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EMAGE:30955

Nrm nurim (nuclear envelope membrane protein) ( MGI:2146855)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002983_01	euxassay_002983_02	euxassay_002983_03	euxassay_002983_04	euxassay_002983_05

euxassay_002983_06	euxassay_002983_07	euxassay_002983_08	euxassay_002983_09	euxassay_002983_10

euxassay_002983_11	euxassay_002983_12	euxassay_002983_13	euxassay_002983_14	euxassay_002983_15

euxassay_002983_16	euxassay_002983_17	euxassay_002983_18	euxassay_002983_19	euxassay_002983_20

euxassay_002983_21	euxassay_002983_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 15 16 17 18 19 20 21 22

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16

orbito-sphenoid

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 12 14 18 19 20 21 22

viscerocranium

moderate

regional

moderate expression: see section 07 08 11 12 14 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4262
Entity Detected:	Nrm, nurim (nuclear envelope membrane protein) ( MGI:2146855)
Sequence:	sense strand is shown >T4262 CTCCCTGGGCAGGGCCAGCAGTCGCGGGAACCCTGAGCCCGCCTTCTCCTCAGGCTCCGCGCTGCTCGCC ATGGCACCGGCGCTGCTGCTGGTTCCTGCGGCCCTCGCCTCCTTCATCCTGGCCTTTGGCACCGGAGTGG AATTCGTGCGTTTCACCTCCCTGAGGCCGCTGCTTGGAGGGATCCCGGAGTCTGGCGGTCCCGATGCCCG CCACGGGTGGTTGGCTGCCCTGCAGGACCGGAGCATTCTTGCCTCCTTGGCTTGGGATCTATGTCTGCTG CTACTGTTTGTGGTACAGCACAGCCTTATGGCGACTGAGGCAGTGAAGGCGTGGACATCCCGGTACTTTG GCGTCCTTCAGAGGTCGCTCTATGTGGCGTGCACAGCCCTGGCCTTGCAGTTGGTGATGCGGTACTGGGA GACTACACCCAGAGGCCCAGTGTTATGGGAGGCTCGGGCTGAACCATGGGCCACCTGGGTACCTCTCCTC TGCTTTGTGCTCCACGTTGTTTCCTGGCTCCTCATCTTTAGCATCCTTCTGGTCTTTGACTACGCTGAAC TGATGGGCCTCAAGCAGGTTTATTACCATGTGCTGGGACTGGGCGAGCCCCTGTCCCTGAAGTCTCCTCG GGCTCTGAGACTCTTTTCCCACCTTCGACACCCAGTGTGTGTGGAGCTGCTGACTGTGCTGTGGGTGGTC CCCACCTTGGGCACCGATCGGCTCCTCCTGGCTCTTCTCTTTACCCTCTACCTGGGCTTGGCTCATGGGC TTGACCAGCAAGATCTCCGCTACCTTCGGTCTCAGCTACAAAGAAAACTCCACCTTCTCTCCAGACCTCA GGATGGGGAAGCAGAGTGAGGAGCTAGTTCTAAGCCCTGAATTTACTTCCCACCTCAAAATCAAATCCCT TAGTATCCAGACGGAGTTTGCTTCAGACCGCGGAGGAGGTTGGAAGTCATGCCCTGAAGGGGCTGTGCCC TCTCCTGCTGGAGAACTCAGTTCCTGAATCTTGATCCTGAATTCTTATTCAAAGTTTCCTCCACTGGTCT TCAAGATCCGCCTCTCACCAGCCAAGAATGGTTACAAAACTCATGAGTCTCCTTTTCTCTTAGAGCATGA CCTCCACACACACTGGGTCAGTTCACACAGGGGAGCACCTCAGCTCACACTGGGGAGCACTCACTCCCCT GGCACTGTTATTGGCTCTGCACCTCAGGGATTCCCTTCCATGTTTCCACCTCAGGGAGCTATGTCAGGGC CTATCTGAAAGTTTATAAGTAGTGGCTCTTTTGTACCGCGGCTGTGCTTCCTCCCCACTCCTGCCTTAGT TTCTCCTTAGGTCTTTCCCTACAGCTCTGTGTGGTCTCCTAAGGCGACGTCTCCCGATTTTAACTCGGGT TGACTCAGGGCTCCCTGCTCTCCCAAAGGATATGCGCTGCAGAAAAAATAAAATTTTATACATATGAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503599 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503599 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002983 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source