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EMAGE:30987

Ube4a ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) ( MGI:2154580)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987
euxassay_002756_01 euxassay_002756_02 euxassay_002756_03 euxassay_002756_04 euxassay_002756_05
EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987
euxassay_002756_06 euxassay_002756_07 euxassay_002756_08 euxassay_002756_09 euxassay_002756_10
EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987
euxassay_002756_11 euxassay_002756_12 euxassay_002756_13 euxassay_002756_14 euxassay_002756_15
EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987 EMAGE:30987
euxassay_002756_16 euxassay_002756_17 euxassay_002756_18 euxassay_002756_19 euxassay_002756_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 15 16
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 03 04 18 19
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 06 07 15
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 15 16 17 18 19 20
vibrissa
moderate moderate
regionalmoderate expression: see section 05 17 18 19 weak expression: see section 03 04
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 15 16 17
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 05 16
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T979
Entity Detected:Ube4a, ubiquitination factor E4A, UFD2 homolog (S. cerevisiae) ( MGI:2154580)
Sequence:sense strand is shown

>T979
TCCTCGAGNCTGTTGGCCTACTGGACCTTTTGGTCGCTGTAGCTGTGAGGTGTCGTCGTCCCTGCCCTTT
CAGGCTCCGGTACTTTTATTTTCATGTGTAAGCTCAATGACTAAGTCTAACCTGGATCTCCACCGAGGTT
CTCAACAGGTGATCTGTGGGCCCTCATTTTTTCCTCCGCTCCCCTAGGGCTGGATGGAGGACACCTTAAA
GTGAAATGACAGACCAGGAGAATAACAACAACATCTCAAGTAACCCCTTTGCTGCTCTTTTTGGCTCCCT
GGCTGATGCCAAGCAGTTTGCAGCAATCCAGAAAGAGCAGCTGAAGCAGCAGTCCGGTAGTGTACTTGGT
TCTGAGGAAGAAGCGTTGTGTAACAGCTTACATGTTCCAGCAGATGAGCTCCCAGCAAGCCCAGATGACT
CGGATAATAGTGTGTCAGAGAGCCTGGATGAGTTTGACTACTCTGTATCTGAGATTAGCCGCTCCTTCCG
GACACGCCAAGAAATGTGTGAGCAACTCAACATCAATCACATGATCCAGAGGATC
Notes:The probe template was PCR amplified from IMAGE:1972320 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1972320 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002756 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS