EMAGE mouse gene expression spatial database

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EMAGE:31000

Mmd2 monocyte to macrophage differentiation-associated 2 ( MGI:1922354)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002991_01	euxassay_002991_02	euxassay_002991_03	euxassay_002991_04	euxassay_002991_05

euxassay_002991_06	euxassay_002991_07	euxassay_002991_08	euxassay_002991_09	euxassay_002991_10

euxassay_002991_11	euxassay_002991_12	euxassay_002991_13	euxassay_002991_14	euxassay_002991_15

euxassay_002991_16	euxassay_002991_17	euxassay_002991_18	euxassay_002991_19	euxassay_002991_20

euxassay_002991_21	euxassay_002991_22	euxassay_002991_23	euxassay_002991_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

cervico-thoracic ganglion

weak

regional

weak expression: see section 10 17

thoracic ganglion

weak

regional

weak expression: see section 14

facial vii ganglion

strong

regional

strong expression: see section 04 05 21 22

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22 23

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 14 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 13 14 15 16

telencephalon marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain marginal layer

strong

regional

strong expression: see section 12 13

cervical ganglion

weak

regional

weak expression: see section 09 17

telencephalon mantle layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 14 15 16 17

olfactory cortex ventricular layer

strong

regional

strong expression: see section 11 12 14 16 17 18 19

ventral grey horn

strong

regional

strong expression: see section 11 12 13 14 15 weak expression: see section 10

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 14 16 17 18 19

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15

pons ventricular layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 07 08 18 19

pons mantle layer

strong

regional

strong expression: see section 06 09 14 16 17 18 19

hypothalamus mantle layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

dorsal root ganglion

strong

regional

strong expression: see section 07 08 09 10 11 12 16 17 18

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13 14 15

liver lobe

strong

regional

strong expression: see section 01 02 03 04 05 06 07 moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 11 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4233
Entity Detected:	Mmd2, monocyte to macrophage differentiation-associated 2 ( MGI:1922354)
Sequence:	sense strand is shown >T4233 GGCTCCGGAGGCAACTGATCGGGTCTGTTCAAGGCCCTGGAGCAGCCCGAGGATCTGACACCTTTCCAAC CTGCTGCGCCTCCCGAAGCGGCTCCTGGTGTTCCCGCGTCCCAGCCCTGGTCTGGGCGCTGATGGAACCG CGGCGATGTTCACTCTGGCCAGGCTGCTGGATTTCCAGAAAACCAAATATGCAAGGTTTATGAATGACCG AGTCCCGGCGCACAAGAGGTACCAGCCCACTGAGTATGAACACGCAGCAAACTGTGCCACCCATGCTTTC TGGATCATTCCCAGCATCCTTGGCAGCTCCAACCTCTACTTCCTGTCCGATGACGACTGGGAGACCATAT CTGCCTGGATCTACGGCCTTGGCCTCTGTGGCCTCTTTGTGGTATCCACCATTTTCCACACAGTCTCATG GAAAAAGAGCCACCTCAGGATGGTAGAGCACTGCCTGCACATGATCGACAGGATGGTGATCTACTTCTTC ATTGCCGCCTCCTATGCTCCTTGGCTGAACCTTCGGGAGCTGGGCCCCTGGGCCTCCCACATGCGCTGGC TGGTGTGGATCATGGCCTCCATCGGTACCATCTATGTCTTTTTCTTCCATGAACGGTACAAGCTTGTGGA GCTGCTTTGCTATGTGGTGATGGGTTTCTTCCCTGCCCTGGTCATCCTCTCCATGCCCAACACTGATGGC ATCTGGGAGCTGATGACAGGAGGGGCTTTCTACTGCCTGGGCATGGTGTTCTTTAAGAGTGATGGAAGAA TTCCCTTTGCCCATGCCATCTGGCACCTCTTTGTGGCATTTGGTGCTGGTACCCACTACTATGCCATCTG GAGGTACCTCTACCTGCCCAGCACACTGCAAACCAAGGTGTCCAAATGAGTGGCCCAGACTCCATGGGTC ATTTGGACTTTGGGATCAAAGCAACATGGGAAGTGAACTGTGCCAAGATCTGCCTGTCTTTTCCTGGATG GAATTCTTCATGGGTCTAAGGAACCTTTCAGTGACAACAAGACCACCTTTTGAATCCCCAACAAGGAAGA AAACATTTAGCCCTTTCTATGGAGGAGAAATTAATTCAGTACTGTGTTTCTACTTTAGACAGTTTTTTTT TTTTTTTTACTATAACCAATATTAACATCTTCAGAAAAGGGCCAAACAGGGAATGACCATCCAGAAGGTA ATTTTTAAAGTTCTGAGGGCCTCCCTTTCTGCTTCAGGAGAAAGTGATTAGCATTATAGATGATCTATGG GGAATATCCCTGACCATGGTAGGCATCACCAATCAATTGCAGCACGCATGTAGCAGTAGAATTCAACATA GTGTCCCATGCAGACATTACTAATTGATTCTGTGCTCATGATGGAACCTTTTGGCTGGCCTGGTGTGTTA GGAGACAGGATCACAGAATAACTATTAGAAGTCACTGTATGTAGGAGCATGCTTATCTCTGGGCACCATT ACCTTCTACTCCCTTCTGTCCATATTTGAGGAAGTTCTTTAAGTGAGCTCTTTGGAGGCAGTGCAGGGAG ACCAAGGCATACATTCCAAACAGGTCCTGACACTCTGTTCCGCCATGGTTGGCTCTTGGTTCTAGAGGAG CTGAGATCTGGGATGAAGATGCTGATACCTGGCCATTTTTTCTCATCTCAAAGAATCTGGAGAAAGGATG CTGTGTTCACCAGAGGGGCTTTTCCTCAGTGTCAGGAAACGGCTCTCGGAGGCCCCTCAGATGGCCCTAG GAGCAGGGTCTTCCTCTATCAGAATGGGTGGAATATCTTCATCCCTCAACCTCTGAAGTGACCTGTCAGC TAAGAGCATGGCTCCCAGGGGCCTGTCCTCAGCCACCTCTATTCCTGGGCTAGCCTCATCCTAAGATGCC ACATGGACACTGGCTGAGTATTGAGCAGCTGGCCTGGCTCCTGACCTGAACTGACATCTCAGACTCTCTG CAAGAGTCTGCTGGAAATCCAAGGTCTTGTTCAACAACTGCCAGCCAGATGGGTGTGGGCAAGGGTGTGC CAAGACAGGGTGCCTTATACTTTGTTCTAGTGCATTCCAAGGTGTTTCAGGGCTTCAGCACCTGGAATCC CATATGTCAAAGCCAGTATTAAATCAAGTTTATGCATTCCTCAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4502289 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4502289 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002991 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source