EMAGE mouse gene expression spatial database

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EMAGE:31006

BC018242 cDNA sequence BC018242 ( MGI:2384575)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002963_01	euxassay_002963_02	euxassay_002963_03	euxassay_002963_04	euxassay_002963_05

euxassay_002963_06	euxassay_002963_07	euxassay_002963_08	euxassay_002963_09	euxassay_002963_10

euxassay_002963_11	euxassay_002963_12	euxassay_002963_13	euxassay_002963_14	euxassay_002963_15

euxassay_002963_16	euxassay_002963_17	euxassay_002963_18	euxassay_002963_19	euxassay_002963_20

euxassay_002963_21	euxassay_002963_22	euxassay_002963_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 16 17 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 20 21

midbrain

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

diencephalon

weak

homogeneous

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21 22

telencephalon marginal layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 04 05 06 18 19 20 21 22 23

telencephalon mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 weak expression: see section 01 02 03 04 05 06 18 19 20 21 22 23

olfactory cortex marginal layer

moderate

regional

moderate expression: see section 08 09 10 13 14 15 16

hindbrain

weak

homogeneous

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord

weak

homogeneous

weak expression: see section 10 11 12 13 14 15 16 17

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4258
Entity Detected:	BC018242, cDNA sequence BC018242 ( MGI:2384575)
Sequence:	sense strand is shown >T4258 GTGTAGTGTTTGGGATGGAGGCCACGCCTGGGTTATATCTTATGTGGGAGTGCCCATATGGGTGACTCTG CACACCTGGAATCTGGTGACCCTCTCTCCAATATGTCATTCTGTGGAGTAGGCCTCAATGGAGCCCTGGA TGCACAGTACCCACCTTCATCTGCCTGGGCCTCAGTTTCCACATCCGTGCAATGGAGATGAATGTTCTTG CCTTGCTGGCCTCACGGAGTGGCTGTGAGTTGGTGGCAGTGGAGGTGGATGCCTGGAAGAAGCCCTAGGG CTCTTTCACAGGCCCCAGCTTCGCCATGGCTGGAGGGAGACCTCACCTGAAGCGGAGTTTCTCTATCATC CCTTGCTTCGTCTTTGTGGAGTCTGTGTTGCTAGGCATCGTGGTCCTTCTTGCGTACCGCCTGGAGTTCA CGGACACCTTCCCTGTGCACACCCAGGGCTTCTTCTGCTATGACAGCGCTTATGCCAAGCCGTATCCCGG GCCTGAGGCTGCCAGCCGAGCGCCCCCTGCCCTCATCTATGCCTTGGTCACTGCTGGGCCCACCCTCACG ATCCTGCTGGGGGAGCTGGCCCGTGCCTTCTTCCCTGCGCCACCCTCCTCCAGTCCTGTCAGTGGGGAGA GCACCATCGTGTCCGGGGCCTGCTGCCGCTTCAGTCCCCCACTGAGGAGGCTGGTCCGTTTCCTGGGGGT GTACTCTTTTGGCCTCTTCACCACGACCATTTTTGCAAATGCGGGACAAGTGGTGACCGGTAACCCCACA CCTCACTTCCTGTCGGTGTGTCGCCCCAACTACACGGCCCTGGGCTGCCCACCACCGTCTCCTGACCGGC CAGGGCCTGACCGCTTCGTCACGGACCAGAGCGCCTGTGCAGGCAGTCCCAGCTTGGTGGCCGCCGCACG CCGCGCCTTTCCCTGCAAGGATGCGGCCCTGTGCGCCTACGCTGTCACCTATACTGCGATGTACGTGACC CTAGTGTTCCGCGTGAAGGGCTCTCGCCTGGTGAAACCTTCCCTCTGCCTGGCCCTGCTGTGCCCCGCCT TCCTGGTGGGCGTGGTCCGCGTGGCGGAGTATCGCAACCATTGGTCGGACGTGCTGGCTGGCTTTCTGAC GGGAGCAGCCATCGCCACCTTTTTGGTCACCTGTGTTGTGCACAATTTCCAGAGCCGACCCCACTCTGGT CGAAGGCTCTCCCCCTGGGAGGACCTGAGCCAGGCCCCCACCATGGACAGCCCCCTCGAAAAGAACCCGA GACCTGCAGGCCGCATTCGACACCGGCACGGCTCACCCCATCCAAGCCGCAGAACTGTGCCCGCCGTGGC CACCTGATTCCCAGCTGTGTGTCCTCCAGGGCGCCAGCTATGTGCTCCTCGCCCCGTGTGCCCCGCCCTC GACTGAGGTCTGAGCCAACACCCCTGCCTCTGCCGCTGCCTCTTCCTGCACCGACTCCCAGTCAGGGTCC CTCGCCTTCCTCCCCTGGACCCGGGGGCCCAGGCGGGGGAGGTGGGCGGGGCCGGAAGCTGCTGCTGCCC ACGCCCCTGCTAAGGGACCTGTACACCCTCAGTGGACTCCATCCCTCCCCTTTCCACCGCGACAACTTCA GCCCTTATCTGTTTGCCAGCCGCGACCACCTGCTGTGAGATCCACCACCTAGAATCTACCCAGTGACCGT TTCCCCCCTGCCACGCGTGTGTGTGGCATGTGAGTGCTAAAGGCCCCTGCCCTCCAAACCAGCCAGGCCC ACCCAGCGTCAGAAGATGGCTGGAAGGGACGCTTCAAGGCAGGGAATCTCTTGGCTGCTCTTTGGCCTTT TGGGATATCTGGGTAGGCAAAAATAACCAGGTGGGCCCATGTCCCCAGGATTTCCCTTTTAACGGCTGAT GCTTGACCTAATTGACCATTGCCTAGCCAGTGAGAGCTCTGAGTTATCAGAATTCAACCCAAAAGTTTAC AAAAGTTTATGGGAACCTCTCACTTCTTAGCTTCCTCAAGCAGGAGGGTGCTACTGTGGCCGGTGTTCAA GTGTCTAAACAGCCAATAAGAGCCCTTGGCGTTCAGAATTGCAGAGAGTGGGTGGGAATGATTCCAGTCA ATGGGGGACCGCCCGTGTCAAAGCCAAGTCAAAGCCAAGGAGGGCTATCCAGCTCTCCTCGGGAACACGG AATCCAGCTGGAGAGGGGGGGGGCAGGCTCCCCCACAGCAGAGTCCTGCGGCACCCCTTCCCTTCTAGAC CTTCCCCCAGGTGCACCATCTCACACCCTCCAACTCCCTACTTAAGTAGGGCTTGCCCCAGTTCACAGGT TTTAGAATTCTGGACGCTGTCTCCCTTGGCTGTGCCTAGGGCACCTCCCCCCACTCCCCGCCAAACCTTC TATTATTACTAGGGCTGTGGAAAATGACTTTATTTTCTCGGGACCCACCCTTCTTCTTCACACTGCCCCC ATGCCTCAGTCTCATGCAGTTGTGCCATCTTGGGGGGCATGGGTGGAGCAGAAGGGGGTTCCCCTACCCT GAATAGCCAAAGGCAGTGGGCAGATGAGACACTGTCCCCCTTTCCTGCCCCCCTCCTCATCTTTAATAAA GACCTGTTTGTAACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4503683 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4503683 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002963 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source