EMAGE mouse gene expression spatial database

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EMAGE:31015

Pigv phosphatidylinositol glycan anchor biosynthesis, class V ( MGI:2442480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001639_01	euxassay_001639_02	euxassay_001639_03	euxassay_001639_04	euxassay_001639_05

euxassay_001639_06	euxassay_001639_07	euxassay_001639_08	euxassay_001639_09	euxassay_001639_10

euxassay_001639_11	euxassay_001639_12	euxassay_001639_13	euxassay_001639_14	euxassay_001639_15

euxassay_001639_16	euxassay_001639_17	euxassay_001639_18	euxassay_001639_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

regional

moderate expression: see section 08 09 12 13 weak expression: see section 10 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5657
Entity Detected:	Pigv, phosphatidylinositol glycan anchor biosynthesis, class V ( MGI:2442480)
Sequence:	sense strand is shown >T5657 GTTGGAGCTCCGCGGGGGTGGTCTGGACGCGCGGGCGCGGAGGATCCGGCGCCAGCTCTGCAACTCTGGA GTCCTCCGGGGAGGGAAGCTGTCAAACGCAGGCCGAGAGAAATTAGGCGGAGGCTGTAGAGAAGCGAGGA GCCCCGCCCCTCCGCCATAGCAGCCTCCGGCGCCGCCTCTTCCTTTGCAGCCTCCGGCCCTCGTCTGCTT CCGGCCCCGTTGCCTGGCCCGGCCCCGCGATTCCCCCGGGACGAAGCCCTGGGCCGTGGCTCCCTTGGGA ATTTAGAAGCCGGAGGAAGCTCAGTCCTGGTGGTGAGAGAGAGGATGGGACTCCTGGACCCATCCCAGAA AGAGGTGCTGAGATTCGCAGTAAACTGCCGCATCCTGACTCTAGTGCTACAGGCACTCTTCAACCTCATC ATCCCAGATCACCACGCAGATGCCTTTTGCCCTCCCCGCCTCGCCCCCTCGGGCTCTGCGGATCAGCTTG TGGAAGGTCTTCTGGGTGGTCTGTCTCGATGGGATGCTGAACACTTCCTGTTTATCGCTGAGCACGGCTA CCTTTATGAGCACAACTTTGCCTTCTTCCCTGGCTTTCCCTTGGCCCTGCTGATGGGGACTGAGCTGCTG AGACCCTTGCAGGGCCTCTTGAGCCAGCGCAGTTGCCTGCTGGTCTCAGTAGCACTTCTCAACTTGCTGT TCTCCGTGCTGGCTGCAGTGGCACTCCATGACTTGGGTTGTCTGGTTCTGCACTGTCCTCGGCAGGCCTT GTGTGCAGCGCTGCTCTTCTGCATCAGTCCTGCCAACGTTTTCCTGGCAGCCGGCTACTCAGAGGCTTTG TTTGCCTTCCTGACGTTCAGCGCCATGGGGCAGCTGGAGAGGGGCCGTGGCTGGGCTAGTGGGCTGCTCT TTGCTCTTGCTGCTGGGGTGCGCTCCAATGGGCTGGTGAGCCTTGGCTTCCTCCTGCACTCCCAGTGCAG AGGCTTTTGTTCCTCTCTGGCGGTGCTGAGCCCCTGGAAGCCCCTCGTGAAGCTGATGGCCTCTGTGTGC TTGTCGGTGCTCATAGTCAGCCTTCCCTTCGCCCTCTTTCAGTATCGTGCCTACATCCAGTTCTGCTCAC CAGGCTCAGCCCCCTCCATCCCTGAGCCCTTGCTGCAGCTAGCTGCCGACAAAGGCTACCGGCTTGCAGG AGAAAATGCACCCCCATGGTGCTCCTGGGACCTTCCTCTAATATACAACTATATTCAGGATGTCTACTGG AATGTTGGTCTTCTGCGATACTATGAGCTCAAACAGGTGCCCAATTTCCTACTAGCTACACCAGTGACAG TACTGGTTGTGTGGGCAACCTGGACATATGTGACCACCCACCCTTGGCTCTGCCTTACACTTGGGCTGCA AAGAACCAAGGACAGGGAGAACCCGGAGAAGCCCCACCGTGGCTTCCTGAGTCCGAAGGTGTTTGTGTAC CTGGTCCACGCTGCAGCACTGCTGGTCTTTGGAGGTCTGTGCATGCATGTCCAGGTTCTCACGCGGTTTT TGGCCTCTTCCACTCCTATCATGTACTGGTTTCCAGCTCACCTGCTTCAGGATCAAGAACCGCTGCTGAG GTGTGTGGACACAGAGCCTGGGAAGCTTCCTCAGGAGAAGTCCCCACCAGGACAGAAGGCCCCCAGAAAC TGTCTCATGAAACTCTTCTACAATTGGAAGAGATGCTCTCCAGTCACAAGATGCGTTCTGGTCTACTTCC TGACCTACTGGCTTCTGGGACTAATTCTCCATTGCAACTTCCTGCCTTGGACTTGACCTGGAGTGTCAAG GGTTGAAAGCTCTAAGACGACACCCTGGCTGGGACAGCCCGCACACTCCGGAGAGCACTGTGCAGTAGGA TCTCCGGGTCTCTCTGGGGGCCAGCTAAGAAGGGAAAATGGGAGCACAGGCGCCCCTTCTCATCCTGGGC GGGGCAGGTGTAGGGGAGTAACTTCAGTGCTCAGTGCAGGGGTCTCAGATCTCCCTAGTCCACAAGTACT AGGCAATCTTAAACCTACTACTGATCCACGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:6528864 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6528864 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001639 same experiment
	EMAGE:31638 same embryo
	EMAGE:31012 same embryo
	EMAGE:29303 same embryo
	EMAGE:29305 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source