EMAGE mouse gene expression spatial database

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EMAGE:31048

Scgn secretagogin, EF-hand calcium binding protein ( MGI:2384873)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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euxassay_002978_06	euxassay_002978_07	euxassay_002978_08	euxassay_002978_09	euxassay_002978_10

euxassay_002978_11	euxassay_002978_12	euxassay_002978_13	euxassay_002978_14	euxassay_002978_15

euxassay_002978_16	euxassay_002978_17	euxassay_002978_18	euxassay_002978_19	euxassay_002978_20

euxassay_002978_21	euxassay_002978_22	euxassay_002978_23	euxassay_002978_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

strong

regional

strong expression: see section 09 10 11 14 15 16

telencephalon mantle layer

strong

regional

strong expression: see section 03 04 05 06 07 17 18 19 20

pituitary gland

strong

regional

strong expression: see section 10 11 12 13 14

olfactory cortex marginal layer

strong

regional

strong expression: see section 08 09 10 11 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4269
Entity Detected:	Scgn, secretagogin, EF-hand calcium binding protein ( MGI:2384873)
Sequence:	sense strand is shown >T4269 GCAGGAGGACGCCTGCGGGTTGCCTCCTGGAGCCTGACATCTAGACTGAAGACTGCTCAGCGACACCAAC GAGCCCGGACGCCCTCGAGCTTGCCTTGCCCACTGGTACCTAGCTAGAGAGGGAGGAAGTGGACCGAAGA GTCGGGAAATACTGCTAGGCGAGTTTACCCACATCAGCACCATGGACAACGCACGCAGAAAAACTCCAGC TCGGCTGGATGCTGCCTGCTTCTGGCAGATCTGGCAGCGCTTCGACAAGGAAGAAAAAGGTTACATAAGA GAGACAGAGCTGGATGCCTTCTTTGATCATTTGCTGGCAAAATCTGGCACTGAGGACACTCTTATGGAAG AAAATGTGCAGAAAGTGAAAGAACAGTTGATGACCAGCCACAACGTCTCTAAGGAAGGCCGCATACTGAT GAAAGAGCTTGCCAGTATGTTCTTATCTGAAGATGAAAATTTCCTCCTGTTCTTTCGCCTGGAAACTCCC TTGGATAACAGTGTAGAGTTTATGCAGATTTGGCGCAAATACGATGCGGACAGCAGTGGTTTCATATCAG CTGCTGAGCTTTGTAACTTTCTTCGAGACCTTTTCCTCCACCACAAAAAGAACATTTCCGAGGCTGAACT GGAAGAGTATACTAGCACCATGATGAAGATCTTTGACAAAAATAAAGATGGACGTTTGGATCTTAATGAC TTGGCAAGGATTCTGGCGCTCCAGGAAAACTTCCTTCTTCAGTTTAAAATGGATGCTTCTTCTACTGAAG AAAGGAAGAGGGACTTTGAGAAAATCTTTGCCCATTACGATGTTAGTAAAACTGGAGCCCTGGAAGGCCC AGAAGTGGATGGATTTGTCAAAGATATGATGGAGCTGGTCCAGCCCAGCATCAGTGGTGTGGATCTGGAT AAGTTCCGGGAGATTCTGCTTCGCCATTGCGATGTCAACAAGGATGGAAAGATCCAGAAGTCTGAGCTGG CACTGTGTCTTGGGCTAAAAATAAACCCCTGATCCCCAACTACTCTGCTCTCCACTTAAGTGTCTGTGTT CCTGCTGTGAGAAGTGTGTTTGTGCTTTGCTCTTGGGGACCCAGTTAATGGACCTTCTCCCTGACAGTTT GGTACTCTTGATGGAGGGAGTGGCCCTAGGGTGCTGCTTCCAGAGGCAGCCATGATCTTGTCCCATGGGA AGCCCCATACAGCTTCTTCCATGTTTGTTCCTTGACCTTGGTCTATCTCCTCAAGGGCCTCATTTTACCT GCTCTGTTCAACCTTCCCTATCGCCTGGTGATCCATTCTACCATCTTCTGCTTGTCCTTGAGCTTTCTGC TCAGTTAGAGTGTCACCTGTATCGTGCTGTCACATGTACAAATATGTCTTCTTGTGGAAATAAAGCTGTG GTCAAGAATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504330 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504330 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002978 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source