EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31052

Oma1 OMA1 homolog, zinc metallopeptidase (S. cerevisiae) ( MGI:1914263)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002976_01	euxassay_002976_02	euxassay_002976_03	euxassay_002976_04	euxassay_002976_05

euxassay_002976_06	euxassay_002976_07	euxassay_002976_08	euxassay_002976_09	euxassay_002976_10

euxassay_002976_11	euxassay_002976_12	euxassay_002976_13	euxassay_002976_14	euxassay_002976_15

euxassay_002976_16	euxassay_002976_17	euxassay_002976_18	euxassay_002976_19	euxassay_002976_20

euxassay_002976_21	euxassay_002976_22	euxassay_002976_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 08 09 10 11 18 19 20 21

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14 15 16 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 13 14 17 weak expression: see section 10 11 12 15 16 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4280
Entity Detected:	Oma1, OMA1 homolog, zinc metallopeptidase (S. cerevisiae) ( MGI:1914263)
Sequence:	sense strand is shown >T4280 AGCAAAAGAAAGATGAGCCTCCTTTATGGACTGCAGTCTACCAGGATAAATCGGTTTCTCTCTGGAGTGA ATAACCTGGCCAACAGGAGACAGTGGACCCCCCCAGCAAGCTGTCCACTGGCACCAAAGCTCCGAGCAGT AAATGCATACTGGGGACTGAACACAGTCAGTCATTGTCATTCAGTGACCTTACTGCCTAGAAACTTTCTT TTCTGTAGGACTCTCAATCACAAAAAATCAAGATGCCTCTCAAGTGCCCAAAGCAAGGAATTGGGGGTGC TTACCTACAGATGTACTGTGCGGGGTGATTCTGTTCTAAGACAAGGAGCAAGGAAAGTGGCTGGCGTTCC TGCTCTCGCGGCCTCCTGTTCTCCAAGCTGTCCTGCCGTAATAGAGGCCCGGAGTTTCCGTACATCTGCA AGGGTTCAGGCTGCCCCAGTCCCTCTCTTGCTGCTCATTCTGAAGCCAGTGCAAAAGCTCCTTGCTATCA TCGTGGGCAGGGGCATAAGGAAATGGTGGCAAGCACTTCCCCCTAACAAGAAGGAGCTATTTAAAGACAG CGTGAGGAAGAACAAGTGGCGGCTGCTTCTTGGTCTGAGTGCATTTGGACTGCTCTTTGTAGTGTTTTAT TTCACTCACCTGGAAGTGAGCCCTGTCACCGGAAGGAGTAAACTACTGTTAGTGGGCAAAGAACACTTCC GACTTCTGTCAGACCTGGAATATGAAGTATGGATGGAAGAATTTAAGAACGATCTGCTGCCTGAGAGAGA CCCCCGCTACCTGACTGTGAAAGAAATGGTTTATCATCTGACACAGTGCAATCGAGACGTCCCGGGGATC TCGGAGACCAACTGGGTGGTTCATGTGGTTGACTCCCCAGCCGTCAATGCCTTTGTGCTCCCAAATGGAC AAGTGTTTATTTTCACCGGGCTTCTGAATAGTGTGACGGACGTGCACCAGCTGTCCTTCCTCCTGGGCCA TGAGATCGCACACGCAGTCCTGGGGCACGCCGCAGAAAAGGCTAGCTTGGTTCATTTACTGGATTTCCTT GGTATGATTTTTCTCACAATGATTTGGGCCATTTGTCCCCGAGACAGCTTGGCAGTTTTGGGCCAGTGGA TACAGTCAAAGTTGCAGGAGTACATGTTTGATCGACCATACAGTAGAACATTGGAGGCCGAAGCTGACAA AGTTGGACTACAGCTGGCTGCAAAGGCATGTGCCGATGTAAGAGCTAGTTCAGTGTTTTGGCAGCAGATG GAGTTCTCAGAGAGTCTTCATGGCTATCCCAAGTTGCCAGAGTGGCTGTCGACACATCCTTCTCATGGGA ACCGAGCTGAGTACTTGGATAGACTCATACCACAGGCTCTCAAACTCCGGGAGGTCTGCAATTGCCCGCC ACTCTCTGGCCCAGACCCTCGGCTATTGTTCAGGCTTACTGTGAAGCGTTTTCTTGAAGACTCGGAGAAA GAGGACCTAAACATCACTGTCAAAAAGCAGAAAACGGATGCTCTCCCCATGCAGAAGCAGGAGCAGATAC CACTGACGTATGTCCTAGAGAAGAGAACTGCAGGCTGAACGGAAGTGGGTGAATCGTGGGCTCCATGAGC AGCACAGTGGACCTTTTCCTTATTACGCAACTTTTAAAAATGATGTTTTCAAGGAAAAAAAAAAAAGAAT GTTCAAAGGCCAAGTCACTTATATTATAGATATTATCTGGATTTTTCTAGGTTTTTATCTTTCATGAAAT ATTAATGTGTTTCCACCTATTTTAAAATACTGCCAATGAGGAAAACATCACAGAATAAATTGATATTATG CATTTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4504835 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4504835 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002976 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source