EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31089

Rad54l ( MGI:894697)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001626_01	euxassay_001626_02	euxassay_001626_03	euxassay_001626_04	euxassay_001626_05

euxassay_001626_06	euxassay_001626_07	euxassay_001626_08	euxassay_001626_09	euxassay_001626_10

euxassay_001626_11	euxassay_001626_12	euxassay_001626_13	euxassay_001626_14	euxassay_001626_15

euxassay_001626_16	euxassay_001626_17	euxassay_001626_18	euxassay_001626_19	euxassay_001626_20

euxassay_001626_21	euxassay_001626_22	euxassay_001626_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 16 17 18 19

thymus primordium

moderate

homogeneous

moderate expression: see section 12 13 14

renal cortex

moderate

regional

moderate expression: see section 06 07 08 09 14 15 16 17 18

vibrissa

moderate

regional

moderate expression: see section 05 06 07 08 19 20 21 22

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16

hypothalamus ventricular layer

strong

regional

strong expression: see section 11 12 13

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15

metencephalon rest of alar plate marginal layer

strong

regional

strong expression: see section 20 21 moderate expression: see section 04 05 07 10 11 12 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 11 12 14 15

metencephalon rest of alar plate ventricular layer

strong

regional

strong expression: see section 20 21 moderate expression: see section 04 05 06 07 15 16 17 18 19

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6971
Entity Detected:	Rad54l, ( MGI:894697)
Sequence:	sense strand is shown >T6971 CCACGCGTCGGGAAGAATAAGGGGAAAGATACAGGAGTCGCTCTCTGCCTCTCTTCAGCTTCTAAGATTC TCCTCCTCCCAACCTGGATTTTTAACTGGTCCTTCCTGGGTTCCTGTTTAACTTCCCACAAGGCGTTCCT AGGTCTCTGCTTGCCTTCCTCACACTGAGGTTTGATGTCGGTCCAGCTCTGGTAAATTCTATCCCTCTCC TAAGAATTCAGGCGTCTCGCCTTCGTACTTAACACTGCACTTTTTTCGTAGGTGTTACTCTCAGGGAAGC AGCCCAGCTAGGGACGTTATTCCAACTCCACTTTGCTCATCTGCAACTTCAATCTGTCTTCTTTCCCATC CTAGATTTAGACCCAGCTCCGCCTGGACTTGGCTTTGTATATTTTGACCTTGAGGAACTTGGGGACCGAC ATTCCTGGTTTGGACCCAAGCCTCATTCCCTGATTGCATCCCCTCCCCTCATACCGACTCTTAAGACAAT ATTTTCAATGATATAGCAACTTCCTTTTATAGCATCCCCTGGTCCTGCATTCTCTGTTGCTGAGTGCTTT TAACTTAGCTGATGGGCACTTGACATCTGATGCTTCTGGGGCCAGGATGAGGAGGAGCTTAGCTCCCAGC CAGTTGGCCAGGAGGAAACCAGAAGACAGATCATCAGATGATGAAGACTGGCAGCCTGGGACAGTAACTC CTAAGAAACGCAAGTCCAGCAGTGAGACCCAGGTCCAGGAGTGTTTCCTGTCTCCTTTTCGGAAGCCCTT GACTCAGCTACTCAACCGGCCACCTTGTCTGGATAGCAGTCAACATGAAGCATTTATTCGAAGTATTTTG TCAAAGCCTTTCAAGGTCCCCATCCCAAATTATCAAGGTCCCCTGGGCTCTCGTGCATTGGGCCTGAAAA GGGTTGGAGTTCGTCGTGCCCTTCATGACCCTCTGGAAGAAGGTGCCTTGGTTCTCTATGAGCCTCCCCC ACTCAGCGCCCATGACCAACTGAAGCTTGACAAGGAAAAACTCCCTGTTCATGTGGTTGTTGATCCTATT CTCAGTAAGGTATTGCGGCCTCATCAGAGAGAGGGAGTGAAGTTCCTATGGGAGTGTGTCACCAGTCGTC GAATTCCTGGAAGCCATGGCTGCATCATGGCTGATGAGATGGGCCTGGGGAAGACACTGCAGTGCATCAC ATTGATGTGGACACTTTTACGCCAGAGCCCAGAATGCAAGCCAGAAATCGAGAAGGCAGTGGTGGTGTCA CCTTCTAGCCTGGTGAAGAACTGGTACAATGAGGTTGAGAAATGGCTTGGAGGGAGGATTCAACCTCTGG CCATCGATGGAGGCTCGAAGGACGAGATAGACCGAAAACTGGAAGGATTCATGAACCAGCGTGGAGCTAG AGTGCCTTCCCCCATCCTCATCATTTCCTATGAGACTTTCCGCCTGCATGTTGGAGTTCTTAAAAAAGGA AATGTTGGACTGGTCATATGTGACGAGGGCCACAGGCTAAAGAACTCTGAGAATCAGACTTACCAGGCTC TGGACAGCTTGAATACCAGCCGGCGGGTGCTAATCTCCGGGACCCCCATCCAAAATGATTTGCTGGAGTA TTTCAGCTTGGTGCACTTTGTAAATTCTGGCATTTTGGGAACTGCCCATGAATTCAAGAAGCATTTTGAG TTGCCAATTTTGAAGAGTCGAGATGCAGCTGCCAGTGAGGCAGACAGGCAGCGTGGGGAGGAGCGTCTGC GGGAGCTCATCGGTATTGTGAACAGGTGCCTGATACGGAGAACATCTGATATCCTCTCTAAGTATCTGCC CGTGAAGATTGAGCAGGTGGTTTGTTGTAGGCTGACACCCCTTCAAACTGAGCTATACAAGAGATTTCTG AGACAGGCTAAGCCTGAAGAAGAATTGCGTGAGGGCAAGATGAGTGTGTCTTCCCTGTCTTCTATCACCT CTCTAAAGAAGCTGTGTAATCATCCAGCTCTAATCTATGACAAGTGTGTGGCAGAGGAGGATGGCTTTGA GGGCACTTTGGGTATCTTCCCACCTGGTTATAACTCTAAAGCTGTAGAGCCACAGTTGTCAGGTAAGATG CTGGTCCTTGATTACATTCTGGCCGTGACTCGAAGCCGTAGCAGTGACAAAGTCGTGCTGGTGTCTAATT ATACTCAGACATTGGATCTCTTTGAAAAGCTGTGCCGGGTTCGAAGGTACTTGTATGTTCGCCTGGATGG CACGATGTCCATTAAGAAGCGAGCCAAGGTTGTGGAGCGCTTCAATAGCCCATCGAGCCCTGATTTTGTT TTCATGCTGAGCAGCAAAGCTGGGGGCTGTGGTCTTAACCTCATTGGTGCTAACCGACTGGTCATGTTTG ATCCTGACTGGAATCCAGCCAATGATGAACAAGCTATGGCCCGAGTCTGGCGTGATGGTCAAAAGAAGAT CTGCTATATCTACCGACTGCTATCTGCAGGAACAATTGAGGAGAAGATCTTTCAGCGGCAGAGCCACAAG AAGGCTCTGAGCAGCTGTGTGGTGGACGAGGAGCAGGATGTGGAGCGCCACTTTTCTCTTGGTGAGCTTA AAGAGCTGTTTACTCTGGATGAAGCAAGCCTCAGTGACACACATGACAGATTGCATTGCCGCCGTTGTGT AAACAACCGTCAGGTCTGGCCACCCCCTGATGGTTCTGACTGCACTTCAGACCTGGCTCAGTGGAACCAC AGCACAGATAAACGAGGGCTCCAGGATGAGGTACTCCAGGCTGCCTGGGATGCTTCATCTACAGCCATCA CCTTCGTCTTCCACCAGCGTTCTCATGAGGAGCAGCGCGGTCTTCACTGATAATCAGCTGGTCTGGATGT AGTTGTTTGGGAAAGGATACAGGGAAAGGGGGTTCTCTGCCTCATGAGGCTCTGCTCAGTTTTATTCTCT GGGAGAAAATCATCAAGAAGGGCTGCATGATGTTTGCCCAAAATTTATTTTATAAGAAAAACTTTTTTGG TTTAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:3987790 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3987790 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001626 same experiment
	EMAGE:31242 same embryo
	EMAGE:31222 same embryo
	EMAGE:29412 same embryo
	EMAGE:31188 same embryo
	EMAGE:29413 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source