EMAGE mouse gene expression spatial database

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EMAGE:31090

Sestd1 ( MGI:1916262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000779_01	euxassay_000779_02	euxassay_000779_03	euxassay_000779_04	euxassay_000779_05

euxassay_000779_06	euxassay_000779_07	euxassay_000779_08	euxassay_000779_09	euxassay_000779_10

euxassay_000779_11	euxassay_000779_12	euxassay_000779_13	euxassay_000779_14	euxassay_000779_15

euxassay_000779_16	euxassay_000779_17	euxassay_000779_18	euxassay_000779_19	euxassay_000779_20

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 15 16 weak expression: see section 17

facial vii ganglion

moderate

homogeneous

moderate expression: see section 03 04 18 19 weak expression: see section 17

vagus x ganglion

weak

homogeneous

weak expression: see section 07

trigeminal v ganglion

moderate

homogeneous

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 weak expression: see section 08 20

vestibulocochlear viii ganglion vestibular component

moderate

homogeneous

moderate expression: see section 04 18

superior glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 05 06 17

inferior glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 05 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T129
Entity Detected:	Sestd1, ( MGI:1916262)
Sequence:	sense strand is shown >T129 AGGACATGGTAGATGTGCGGAGGCTGAAGATGCTTCAGATGGTGCAGTTGTTTAAGTGTGAAGAGGATGC TTCACAGGCAGTAGAATGGCTGAGTGAACTTCTGGATGCCCTGCTGAAGACCCATATCAGGCTGGGTGAC GATGCTCAGGAAACAAAGGTTTTACTGGAAAAACACAGAAAATTTGTCGATGTTGCCCAGAGTACTTACG ACTATGGCAGACAGCTGCTGCAGGCCACAGTTGTACTGTGCCAGTCTCTGCGCTGCACTTCCCGGTCCTC AGGGGACACACTTCCTCGACTGAACAGAGTGTGGAAGCAGTTTACAGTCGCATCTGAAGAGAGAGTGCAC AGGTTGGAGATGGCTATTGCCTTTCACTCCAATGCCGAAAAGATTTTGCAAGACTGCCCAGAAGAGCCTG AAGCAATGAATGATGAGGAACAGTTTGAGGAAATTGAAGCAATTGGAAAATCACT
Notes:	The probe template was PCR amplified from IMAGE:2581948 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2581948 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000779 same experiment
	EMAGE:30270 same embryo
	EMAGE:30263 same embryo
	EMAGE:29333 same embryo
	EMAGE:31038 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source