EMAGE mouse gene expression spatial database

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EMAGE:31100

Reep2 receptor accessory protein 2 ( MGI:2385070)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002920_01	euxassay_002920_02	euxassay_002920_03	euxassay_002920_04	euxassay_002920_05

euxassay_002920_06	euxassay_002920_07	euxassay_002920_08	euxassay_002920_09	euxassay_002920_10

euxassay_002920_11	euxassay_002920_12	euxassay_002920_13	euxassay_002920_14	euxassay_002920_15

euxassay_002920_16	euxassay_002920_17	euxassay_002920_18	euxassay_002920_19	euxassay_002920_20

euxassay_002920_21	euxassay_002920_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 18 19

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 17

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 08 19 moderate expression: see section 07

thoracic ganglion

moderate

regional

moderate expression: see section 14

testis

strong

regional

strong expression: see section 08 09 moderate expression: see section 06 07 20 21 22

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 17 18 19

facial vii ganglion

strong

regional

strong expression: see section 04 05 06 20 21

cornea stroma

strong

regional

strong expression: see section 01 02 03

vagus x ganglion

strong

regional

strong expression: see section 09 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

cervical ganglion

moderate

regional

moderate expression: see section 10 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4285
Entity Detected:	Reep2, receptor accessory protein 2 ( MGI:2385070)
Sequence:	sense strand is shown >T4285 CCCCAAAGCCGCTGCCGCTGCTAACAGCGGCTCCTGCTGCCTCAGCCGCCGGCCTCGGGCAGCTGCATCC ATACCCCGGGCTGGGTCCCCGCCCCGCCATGGTGTCCTGGATCATCTCTCGCCTGGTGGTGCTCATCTTT GGCACCCTGTACCCAGCCTATTCTTCCTACAAGGCCGTGAAGACCAAAAACGTGAAGGAATACGTAAAAT GGATGATGTATTGGATAGTCTTCGCCTTCTTCACCACAGCTGAGACACTTACAGATATAATACTGTCCTG GTTCCCCTTCTACTTTGAGCTCAAGATTGCCTTTGTGATATGGCTGTTGTCCCCTTACACCAAGGGCTCC AGTGTCCTCTACCGCAAGTTCGTGCACCCAACACTGTCCAACAAGGAAAAGGAGATCGACGAATACATCA CACAAGCTCGAGACAAGAGCTATGAGACGATGATGAGGGTGGGCAAGAGGGGCCTGAACCTGGCTGCCAA TGCTGCAGTCACAGCTGCTGCCAAGGGCCAGGGGGTGCTGTCGGAAAAGCTGCGGAGCTTCAGCATGCAG GACCTGACTCTCATTCGAGATGAGGATGCGTTACCGCTGCAGGGGCCAGATGGCCGCCTCCAACCCGGCC CCGTGGGTCTCCTGGACACTATTGAGGACTTAGGAGATGAGCCTGCCCTAAGTCTAAGGTCTAGCACAAG CCAGCCAGATCCCCGGACAGAGACCTCAGAAGATGACCTGGGAGACAAGGCACCCAAGAGGACCAAACCT ATCAAAAAAGTACCCAGAGCTGAGCCGCCGGCTTCCAAGACACTGAAGACCCGGCCCAAGAAGAAGAGTT CTGGAGGGGGCGACTCAGCATGAGAGGCTCGGCTCCCCGCACCCCTCCATCTCTAGGACAAGCACGGAGA AGAGCTGCAGCCCTGGCCCCTCCAACAGTGAGCCACTAGCCCCAAGTGTTTCAGACCCGCAAACCCCCCA GGTGGCTGTCCCCAGCACCCCCTTCCCATGAACATGCCTCCGGAGGGGGCTTGGCAAAGAGGAGGTTGAG GACAGAGGTGATGACTAAGCCCTTGAGGAGGTGGAGGCTCCGTGGCCAGCCAGCGCTGCTCTTTTTGCTG CTCCAGTCCCACGCTGCCCATCCAGTGCCTTGCTGACCATGGCCGTCCCCTCTCAGAGGCCTAACCTGCT TCCCCCACCCCCAGGGAGGGGACCTTGAGAAAGGGAAGAAACTGGGCAGGCCTGGGAGGAGGGTGTTCCT CAGGCGCAGCTATGCATGGAGACAAGGGCTTTGAATAAGCAGCACGGCCAGGCCAGCATTGCAGCTGGTA GGGCTGTGTATTCTACCAAAAGAGAGGTGAGATGCTAAGGGTGTGCGTGTTAGGGAGGTTGGGTGAGAGG GGTCACCTCCTCCCTACATCAGCTCTGGTCTTTCTTCCTCTTTATCCACCCCTGCTGGTCTCTGAACCTC AGAACTCAAGCCCTTGACAGAACGAGGCTCCCAGGGACTCCTCCCTACCCTTCCATCCTTGATCCCTTTT ACACATTCATGCCCACGTAAGCTGGACTCCTCTGCTCCTAGGCTCCATCACAGAGGGCCTGCCAAGATGG CTCCTTTCCTCTCCTGTAAAGGGGATGCATCCCACCTTGCCATGGGGTCCCCTTCCTGTGCCAAGCAAAC CCTGGTCAGAACTAACCTGGACCCCACCCCTGGCGCTGGTCCTGCCAGCCTCAATGGGGCCAAGTGGTAC CTGTGCAGGGAAGGAGATGCAGGAGCTGGGAGGCTCCCAACTCCACGGTGCCCACACTGGACCTTGGCAC TGGATGAGCCAATAAACGAAACTCTGACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4505679 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4505679 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002920 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source