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EMAGE:31120

Ugt1a6a UDP glucuronosyltransferase 1 family, polypeptide A6A ( MGI:2137698)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120
euxassay_006282_01 euxassay_006282_02 euxassay_006282_03 euxassay_006282_04 euxassay_006282_05
EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120
euxassay_006282_06 euxassay_006282_07 euxassay_006282_08 euxassay_006282_09 euxassay_006282_10
EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120
euxassay_006282_11 euxassay_006282_12 euxassay_006282_13 euxassay_006282_14 euxassay_006282_15
EMAGE:31120 EMAGE:31120 EMAGE:31120 EMAGE:31120
euxassay_006282_16 euxassay_006282_17 euxassay_006282_18 euxassay_006282_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
lateral ventricle choroid plexus
moderate moderate
regionalmoderate expression: see section 06 07 09 15 16 weak expression: see section 08 10 11 12 14
4th ventricle
moderate moderate
regionalmoderate expression: see section 07 09 16 weak expression: see section 02 03 04 05 06 08 10 11 12 14 15 17
stomach
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6289
Entity Detected:Ugt1a6a, UDP glucuronosyltransferase 1 family, polypeptide A6A ( MGI:2137698)
Sequence:sense strand is shown

>T6289
GGCCTCAGTTCTAGGTGACAAGCTGCTGGTGGTCCCCCAGGATGGAAGCCACTGGCTTAGCATGAAGGAG
ATAGTAGAACATCTCAGTGAACGAGGACATGACATTNTGGTCCTAGTGCCAGAAGTCAATTTGCTTTTGG
GAGAATCCAAATACTACAGGAGGAAAATCTTCTCAGTTACCTACAGCCTAGAAGAACTGCAGACCCGTTT
TCGCACCTTTGGAAACAACCACTTTNTTCCCGGTGCCNCCCTGATGGGTCCTCTAAGAGAGTACAGGAAC
AACATGATTGNCNTGGACATGTTCTTTTCCAACTGCCAGAGCCTCCTGAAGGACTCTGCCACCCTCAGCT
TCCTCAGGGAGAACAAGTTTGATGCTCTGTTCACAGACCCAGCCATGCCCTGTGGTGTGATCCTGGCTGA
GTATCTCAACCTGCCCTCTGTCTACCTCTTCAGAGGTTTCCCATGCTCTCTGGAACACATGCTTGGTCAA
AGCCCAAGCCCTGTGTCCTATGTGCCCAGATTCTACACCAAATTCTCAGACCACATGACGTTTCCCCAAC
GGCTGGCCAACTTCATTGTTAACATCTTGGAAAACTACCTATATTATTGTCTGTATTCAAAGTATGA
Notes:The probe template was PCR amplified from E14.5 mouse embryo cDNA using gene specific primers.. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006282 same experiment
 EMAGE:29272 same embryo
 EMAGE:29271 same embryo
 EMAGE:29235 same embryo
 EMAGE:29237 same embryo
 EMAGE:31336 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS